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1. 发明申请

US20100291644A1 PROCESS FOR PREPARING METHACRYLIC ACID OR METHACRYLIC ESTERS 有权
标题翻译：制备甲基丙烯酸或甲基丙烯酸酯的方法
公开(公告)号：US20100291644A1
公开(公告)日：2010-11-18
申请号：US12602593
申请日：2008-05-30
申请人： Achim Marx , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Birgit Alber , Georg Fuchs , Lothar Eggeling
发明人： Achim Marx , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Birgit Alber , Georg Fuchs , Lothar Eggeling
IPC分类号： C12P7/62 , C12P7/40 , C12P7/44
CPC分类号： C12P7/42 , C08F120/06 , C08F120/10 , C12N9/0008 , C12N9/001 , C12N9/1096 , C12N9/16 , C12N9/88 , C12P7/40 , C12P7/52 , C12P7/625 , C12Y102/04004 , C12Y103/99012 , C12Y206/01042 , C12Y301/02004 , C12Y402/01017
摘要： The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of IA) preparation of 3-hydroxyisobutyric acid by a process comprising the process step of bringing a cell which has been genetically modified in comparison with its wild type in such a way that it is capable of forming more 3-hydroxyisobutyric acid, or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid in comparison with its wild type, into contact with a nutrient medium comprising, as carbon source, carbohydrates, glycerol, carbon dioxide, methanol, L-valine or L-glutamate under conditions under which 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid are formed from the carbon source, if appropriate, isolation of the 3-hydroxyisobutyric acid from the nutrient medium and also, if appropriate, neutralization of the 3-hydroxyisobutyric acid, IB) dehydration of the 3-hydroxyisobutyric acid with formation of methacrylic acid and also, where appropriate, esterification methacrylic acid. The invention also relates to a process for the preparation of polymethacrylic acid or polymethacrylic esters.
摘要翻译：本发明涉及一种制备甲基丙烯酸或甲基丙烯酸酯的方法，其包括以下工艺步骤：IA）通过包括以下步骤的方法制备3-羟基异丁酸，所述方法包括将经遗传修饰的细胞与其野生型，使得其能够形成更多的3-羟基异丁酸或与其野生型相比基于3-羟基异丁酸的聚羟基链烷酸酯与营养培养基接触，所述营养培养基包含作为碳源的碳水化合物，甘油，碳二氧化物，甲醇，L-缬氨酸或L-谷氨酸盐，其中3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸酯由碳源形成的条件下，如果合适，则从营养培养基中分离出3-羟基异丁酸，如果适当，中和3-羟基异丁酸，IB）3-羟基异丁酸脱水形成甲基丙烯酸酸，并且在适当的情况下酯化甲基丙烯酸。本发明还涉及一种制备聚甲基丙烯酸或聚甲基丙烯酸酯的方法。

2. 发明申请

US20150218601A1 MICROBIOLOGICAL PRODUCTION OF 3-HYDROXYISOBUTYRIC ACID 审中-公开
标题翻译： 3-羟基丁酸的微生物生产
公开(公告)号：US20150218601A1
公开(公告)日：2015-08-06
申请号：US14624713
申请日：2015-02-18
申请人： Achim MARX , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Georg Fuchs , Birgit Alber , Lothar Eggeling
发明人： Achim MARX , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Georg Fuchs , Birgit Alber , Lothar Eggeling
IPC分类号： C12P7/42 , C12N9/88 , C08F120/10 , C12N9/10 , C08F120/06 , C12N9/16 , C12N9/02
CPC分类号： C12P7/42 , C08F120/06 , C08F120/10 , C12N9/0008 , C12N9/001 , C12N9/1096 , C12N9/16 , C12N9/88 , C12P7/40 , C12P7/52 , C12P7/625 , C12Y102/04004 , C12Y103/99012 , C12Y206/01042 , C12Y301/02004 , C12Y402/01017
摘要： The present invention relates to a cell which has been modified in comparison with its wild type in such a way that it is capable of forming more, by comparison with its wild, 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid via methylmalonate-semialdehyde or 3-hydroxybutyryl-coenzyme A as precursors. The invention also relates to a method of generating a genetically modified cell, to the genetically modified cell obtainable by these methods, to a method of producing 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid, to a method of producing methacrylic acid or methacrylic esters, and to a method of producing polymethacrylic acid or polymethacrylic esters. The present invention furthermore relates to an isolated DNA, to a vector, to the use of this vector for transforming a cell, to a transformed cell, and to a polypeptide.
摘要翻译：本发明涉及与其野生型相比已被修饰的细胞，使得其能够通过与其野生型3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸通过甲基丙二酸酯 - 半醛或3-羟基丁酰辅酶A作为前体。本发明还涉及生产基于3-羟基异丁酸的3-羟基异丁酸或聚羟基链烷酸酯的生产方法，该方法可以通过这些方法产生遗传修饰的细胞，生产甲基丙烯酸或其制备方法甲基丙烯酸酯，以及生产聚甲基丙烯酸或聚甲基丙烯酸酯的方法。本发明还涉及分离的DNA，载体，使用该载体转化细胞，转化细胞和多肽。

3. 发明授权

US09234218B2 Process for preparing methacrylic acid or methacrylic esters 有权
标题翻译：制备甲基丙烯酸或甲基丙烯酸酯的方法
公开(公告)号：US09234218B2
公开(公告)日：2016-01-12
申请号：US12602593
申请日：2008-05-30
申请人： Achim Marx , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Birgit Alber , Georg Fuchs , Lothar Eggeling
发明人： Achim Marx , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Birgit Alber , Georg Fuchs , Lothar Eggeling
IPC分类号： C12P7/42 , C12P7/52 , C12N9/02 , C12P7/40 , C12P7/62 , C08F120/06 , C08F120/10 , C12N9/10 , C12N9/16 , C12N9/88
CPC分类号： C12P7/42 , C08F120/06 , C08F120/10 , C12N9/0008 , C12N9/001 , C12N9/1096 , C12N9/16 , C12N9/88 , C12P7/40 , C12P7/52 , C12P7/625 , C12Y102/04004 , C12Y103/99012 , C12Y206/01042 , C12Y301/02004 , C12Y402/01017
摘要： The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of IA) preparation of 3-hydroxyisobutyric acid by a process comprising the process step of bringing a cell which has been genetically modified in comparison with its wild type in such a way that it is capable of forming more 3-hydroxyisobutyric acid, or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid in comparison with its wild type, into contact with a nutrient medium comprising, as carbon source, carbohydrates, glycerol, carbon dioxide, methanol, L-valine or L-glutamate under conditions under which 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid are formed from the carbon source, if appropriate, isolation of the 3-hydroxyisobutyric acid from the nutrient medium and also, if appropriate, neutralization of the 3-hydroxyisobutyric acid, IB) dehydration of the 3-hydroxyisobutyric acid with formation of methacrylic acid and also, where appropriate, esterification methacrylic acid. The invention also relates to a process for the preparation of polymethacrylic acid or polymethacrylic esters.
摘要翻译：本发明涉及一种制备甲基丙烯酸或甲基丙烯酸酯的方法，其包括以下工艺步骤：IA）通过包括以下步骤的方法制备3-羟基异丁酸，所述方法包括将经遗传修饰的细胞与其野生型，使得其能够形成更多的3-羟基异丁酸或与其野生型相比基于3-羟基异丁酸的聚羟基链烷酸酯与营养培养基接触，所述营养培养基包含作为碳源的碳水化合物，甘油，碳二氧化物，甲醇，L-缬氨酸或L-谷氨酸盐，其中3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸酯由碳源形成的条件下，如果合适，则从营养培养基中分离出3-羟基异丁酸，如果适当，中和3-羟基异丁酸，IB）3-羟基异丁酸脱水形成甲基丙烯酸酸，并且在适当的情况下酯化甲基丙烯酸。本发明还涉及一种制备聚甲基丙烯酸或聚甲基丙烯酸酯的方法。

4. 发明申请

US20100068773A1 MICROBIOLOGICAL PRODUCTION OF 3-HYDROXYISOBUTYRIC ACID 审中-公开
标题翻译： 3-羟基丁酸的微生物生产
公开(公告)号：US20100068773A1
公开(公告)日：2010-03-18
申请号：US12303161
申请日：2007-06-01
申请人： Achim Marx , Markus Poetter , Stefen Buchholz , Alexander May , Hermann Siegert , Georg Fuchs , Birgit Alber , Lothar Eggeling
发明人： Achim Marx , Markus Poetter , Stefen Buchholz , Alexander May , Hermann Siegert , Georg Fuchs , Birgit Alber , Lothar Eggeling
IPC分类号： C12P7/62 , C12N1/21 , C12P7/42 , C07H21/04 , C12N15/74 , C07K14/00
CPC分类号： C12P7/42 , C08F120/06 , C08F120/10 , C12N9/0008 , C12N9/001 , C12N9/1096 , C12N9/16 , C12N9/88 , C12P7/40 , C12P7/52 , C12P7/625 , C12Y102/04004 , C12Y103/99012 , C12Y206/01042 , C12Y301/02004 , C12Y402/01017
摘要： The present invention relates to a cell which has been modified in comparison with its wild type in such a way that it is capable of forming more, by comparison with its wild, 3-hydroxyisobutyric acid or poly-hydroxyalkanoates based on 3-hydroxyisobutyric acid via methylmalonate-semialdehyde or 3-hydroxybutyryl-coenzyme A as precursors. The invention also relates to a method of generating a genetically modified cell, to the genetically modified cell obtainable by these methods, to a method of producing 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid, to a method of producing methacrylic acid or methacrylic esters, and to a method of producing polymethacrylic acid or polymethacrylic esters. The present invention furthermore relates to an isolated DNA, to a vector, to the use of this vector for transforming a cell, to a transformed cell, and to a polypeptide.
摘要翻译：本发明涉及与其野生型相比被修饰的细胞，使得其能与其野生型3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸相比形成更多的细胞甲基丙二酸酯 - 半醛或3-羟基丁酰辅酶A作为前体。本发明还涉及生产基于3-羟基异丁酸的3-羟基异丁酸或聚羟基链烷酸酯的生产方法，该方法可以通过这些方法产生遗传修饰的细胞，生产甲基丙烯酸或其制备方法甲基丙烯酸酯，以及生产聚甲基丙烯酸或聚甲基丙烯酸酯的方法。本发明还涉及分离的DNA，载体，使用该载体转化细胞，转化细胞和多肽。

5. 发明申请

US20100190224A1 ENZYME FOR THE PRODUCTION OF METHYLMALONYL-COENZYME A OR ETHYLMALONYL-COENZYME A AND USE THEREOF 审中-公开
标题翻译：用于生产甲基亚甲基二甲基丙烯酸酯或其乙烯基 - 苯甲醚的酶及其用途
公开(公告)号：US20100190224A1
公开(公告)日：2010-07-29
申请号：US12593090
申请日：2008-03-27
申请人： Markus Poetter , Achim Marx , Georg Fuchs , Birgit Alber , Tobias Juergen Erb
发明人： Markus Poetter , Achim Marx , Georg Fuchs , Birgit Alber , Tobias Juergen Erb
IPC分类号： C12P7/42 , C12N15/74 , C12N9/00 , C12N1/21 , C12N15/63 , C07H21/04
CPC分类号： C12P7/52 , C12N9/506 , C12P7/42
摘要： The present invention relates to an isolated DNA, which is selected from the following sequences: a) a sequence according to SEQ ID No. 01, b) an intron-free sequence that is derived from a sequence according to a) and encodes the same protein or peptide as the sequence according to SEQ ID No. 01, c) a sequence that encodes a protein or peptide, which comprises the amino acid sequence according to SEQ ID No. 02, d) a sequence that is at least 80% identical to a sequence according to a) to c), e) a sequence that hybridizes with the antisense strand of a sequence according to one of the groups a) to d) or would hybridize taking into account degeneration of the genetic code, f) a derivative of a sequence according to one of the groups a) to e) obtained by substitution, addition, inversion and/or deletion of one or more bases, g) a sequence that corresponds to SEQ ID No. 01 within the degeneration of the genetic code, h) a sequence with neutral sense mutations of SEQ ID No. 01, and i) a sequence complementary to a sequence according to one of the groups a) to h). The invention further relates to a vector, the use of this vector for transformation of a cell, a transformed cell, a polypeptide, cells genetically engineered relative to their wild type, a method of production of a genetically engineered cell, the genetically engineered cell obtainable by this method, the use of this cell and a method of production of 3-hydroxyisobutyric acid or of a derivative thereof.
摘要翻译：本发明涉及分离的DNA，其选自以下序列：a）根据SEQ ID No 01的序列，b）源自根据a）的序列的内含子序列，并编码它们蛋白质或肽，其为编码蛋白质或肽的序列，其包含根据SEQ ID No.22的氨基酸序列，d）至少80％相同的序列根据a）至c）的序列，e）与根据a）至d）中的一个组的序列的反义链杂交的序列或将考虑到遗传密码的变性而杂交，f）a 根据通过一个或多个碱基的取代，添加，倒位和/或缺失得到的a）至e）中的一个序列的序列的衍生物，g）在遗传变性中对应于SEQ ID No 01的序列代码，h）具有SEQ ID No 01的中性有义突变的序列，和i）与根据a）至h）中的一个组合的序列互补的序列。本发明进一步涉及载体，该载体用于转化细胞，转化细胞，多肽，相对于其野生型基因工程改造的细胞，遗传工程细胞的生产方法，可获得的遗传工程改造的细胞通过该方法，可以使用该细胞和3-羟基异丁酸或其衍生物的制造方法。

6. 发明授权

US10174349B2 Recombinant cell producing 2-hydroxyisobutyric acid 有权
公开(公告)号：US10174349B2
公开(公告)日：2019-01-08
申请号：US13001204
申请日：2009-04-28
申请人： Liv Reinecke , Steffen Schaffer , Achim Marx , Markus Poetter , Thomas Haas
发明人： Liv Reinecke , Steffen Schaffer , Achim Marx , Markus Poetter , Thomas Haas
IPC分类号： C12P7/42 , C12P7/62
摘要： The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor.

7. 发明申请

US20110171702A1 RECOMBINANT CELL PRODUCING 2-HYDROXYISOBUTYRIC ACID 审中-公开
标题翻译：重组细胞生产2-羟基丁酸
公开(公告)号：US20110171702A1
公开(公告)日：2011-07-14
申请号：US13001204
申请日：2009-04-28
申请人： Liv Reinecke , Steffen Schaffer , Achim Marx , Markus Poetter , Thomas Haas
发明人： Liv Reinecke , Steffen Schaffer , Achim Marx , Markus Poetter , Thomas Haas
IPC分类号： C12P7/40 , C12N5/10 , C12N1/00 , C12P7/42 , C12P7/62
CPC分类号： C12P7/625 , C12P7/42
摘要： The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor.
摘要翻译：本发明涉及一种经遗传修饰以能够产生比其野生型更多的2-羟基异丁酸或更多含2-羟基异丁酸单体单元的聚羟基链烷酸酯的细胞，其特征在于2-羟基异丁酸或含有2-羟基异丁酸的聚羟基链烷酸酯，通过作为中间体的乙酰乙酰辅酶A和作为前体的3-羟基丁酰辅酶A制备羟基异丁酸单体单元。

8. 发明申请

US20140308717A1 OXIDATION AND AMINATION OF SECONDARY ALCOHOLS 审中-公开
标题翻译：二次醇的氧化和胺化
公开(公告)号：US20140308717A1
公开(公告)日：2014-10-16
申请号：US14237121
申请日：2012-07-27
申请人： Thomas Haas , Markus Poetter , Jan Christoph Pfeffer , Wolfgang Kroutil , Arne Skerra , Alexandra Lerchner , Katharina Christin Tauber , Johann H. Sattler , Steffen Schaffer
发明人： Thomas Haas , Markus Poetter , Jan Christoph Pfeffer , Wolfgang Kroutil , Arne Skerra , Alexandra Lerchner , Katharina Christin Tauber , Johann H. Sattler , Steffen Schaffer
IPC分类号： C12P13/00 , C12P17/04 , C12N15/63
CPC分类号： C12P13/001 , C12N9/0006 , C12N9/1096 , C12N15/63 , C12P13/005 , C12P13/04 , C12P17/04 , C12Y101/01001 , C12Y206/01 , C12Y206/01018
摘要： The present invention relates to a method comprising the steps a) providing a secondary alcohol, b) oxidizing the secondary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase and c) contacting the oxidation product of step a) with a transaminase, wherein the NAD(P)+-dependent alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a secondary alcohol.
摘要翻译：本发明涉及包括以下步骤的方法：a）提供仲醇，b）通过使其与NAD（P）+依赖性醇脱氢酶接触来氧化仲醇，和c）将步骤a）的氧化产物与转氨酶，其中NAD（P）+依赖性醇脱氢酶和/或转氨酶是重组或分离的酶，用于实施该方法的全细胞催化剂，以及使用这种全细胞催化剂来氧化二级醇。

9. 发明授权

US09315443B2 Liquid cation exchanger 有权
标题翻译：液体阳离子交换剂
公开(公告)号：US09315443B2
公开(公告)日：2016-04-19
申请号：US14000067
申请日：2011-12-01
申请人： Frank Erhardt , Thomas Haas , Martin Roos , Daniel Demicoli , Markus Poetter , Anja Schubert , Jan Christoph Pfeffer , Thomas Tacke , Harald Haeger , Andreas Pfennig , Marie-Dominique Przybylski-Freund
发明人： Frank Erhardt , Thomas Haas , Martin Roos , Daniel Demicoli , Markus Poetter , Anja Schubert , Jan Christoph Pfeffer , Thomas Tacke , Harald Haeger , Andreas Pfennig , Marie-Dominique Przybylski-Freund
IPC分类号： C07C227/00 , C07C67/62 , B01D11/04 , B01J39/04 , B01J39/16 , C07C227/40 , C07C229/08 , C02F1/26 , C02F1/42 , C02F101/34
CPC分类号： C07C67/62 , B01D11/0492 , B01J39/04 , B01J39/16 , C02F1/26 , C02F2001/425 , C02F2101/34 , C07C227/40 , C07C229/08
摘要： The invention relates to a method for removing an organic compound having one or more positive charges from an aqueous solution. Said method consists of the following steps a) the aqueous solution containing the organic compound, and a hydrophobic organic solution which contains a hydrophobic liquid cation exchanger having one or more negative charges and a negative total charge, are provided, b) the aqueous solution and the organic solution are brought into contact with each other and c) the organic solution is separated from the aqueous solution.
摘要翻译：本发明涉及从水溶液中除去具有一个或多个正电荷的有机化合物的方法。所述方法包括以下步骤a）含有有机化合物的水溶液和含有具有一个或多个负电荷和负总电荷的疏水液体阳离子交换剂的疏水性有机溶液，b）水溶液和使有机溶液彼此接触并且c）将有机溶液与水溶液分离。

10. 发明授权

US09249435B2 Process for the improved separation of a hydrophobic organic solution from an aqueous culture medium 有权
标题翻译：改进疏水性有机溶液与水性培养基分离的方法
公开(公告)号：US09249435B2
公开(公告)日：2016-02-02
申请号：US13721481
申请日：2012-12-20
申请人： Jasmin Gielen , Thomas Haas , Hans-Georg Hennemann , Steffen Schaffer , Mirja Wessel , Katharina Berse , Marion Schiebelhut , Sabine Hafkemeyer , Markus Poetter , Frank Erhardt
发明人： Jasmin Gielen , Thomas Haas , Hans-Georg Hennemann , Steffen Schaffer , Mirja Wessel , Katharina Berse , Marion Schiebelhut , Sabine Hafkemeyer , Markus Poetter , Frank Erhardt
IPC分类号： C12P13/04 , C12P7/64 , C12N9/02
CPC分类号： C12P7/6436 , C12N9/001 , C12P13/04 , C12Y103/99003
摘要： A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyzes one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyzes one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.
摘要翻译：提供了一种从水性培养基中改善疏水性有机溶液分离的方法。该方法包括制备活性降低的代谢活性细胞的水性培养基; 使含水培养基与包含用于生物转化的底物的疏水性有机溶液接触; 进行基底的生物转化; 并从含水培养基中分离包含生物转化底物的疏水性有机溶液。与野生型活性细胞相比，代谢活性细胞的活性降低，活性降低至少是催化脂肪酸一次反应的一种酶。本发明进一步提供了一种代谢活性细胞，其与野生型相比具有降低的活性，其酶催化脂肪酸的一种反应的反应，包括选自FadA，FadB，FadD的酶，FadL和FadE及其变体。

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