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2. 发明申请

US20100197888A1 Method for Manufacturing Linear Polyethylenimine (PEI) for Transfection Purpose and Linear PEI Obtained with Such Method 审中-公开
标题翻译：制造用于转染目的的线性聚乙烯亚胺（PEI）和用这种方法获得的线性PEI的方法
公开(公告)号：US20100197888A1
公开(公告)日：2010-08-05
申请号：US12671312
申请日：2008-07-31
申请人： Abdennaji Adib , Fabrice Stock , Patrick Erbacher
发明人： Abdennaji Adib , Fabrice Stock , Patrick Erbacher
IPC分类号： C08G73/06
CPC分类号： C08G73/0233 , C12N15/87
摘要： The invention concerns a method of synthesising and preparing linear polyethylenimine (PEI) for use as a transfection vector, and the product obtained with such a method. It comprises drying a monomer 2-ethyl-2-oxazoline and polymerising said monomer for obtaining poly (2-ethyl-2-oxazoline) (PEOX) by: using acetonitrile as solvent, adding a dried initiator of the reaction of polymerisation, and mixing them altogether, purifying said obtained PEOX by evaporation, while performing at least three times successive washing/precipitation steps with methanol and diethyl ether and corresponding filtrations, in order to obtain (i), by performing 1H-NMR tests, correct identification of said PEOX polymer, confirmation of absence of monomer to a level 23,000 Da and polydispersity (Mw/Mn) of said PEOX
摘要翻译：本发明涉及一种合成和制备用作转染载体的线性聚乙烯亚胺（PEI）的方法，以及用这种方法获得的产品。它包括干燥单体2-乙基-2-恶唑啉并使所述单体聚合以获得聚（2-乙基-2-恶唑啉）（PEOX）：通过使用乙腈作为溶剂，加入干燥的聚合反应引发剂和混合它们一起通过蒸发纯化所获得的PEOX，同时用甲醇和二乙醚进行至少三次连续的洗涤/沉淀步骤和相应的过滤，以获得（i）通过进行1H-NMR测试，正确鉴定所述PEOX 聚合物，确认不存在单体至<1.0％的水平，并且确认溶剂不存在至<5.0％的水平，和（ii）通过进行凝胶渗透色谱法，分子量（Mw）> 23,000Da的平均值和多分散性所述PEOX <1.5的Mw / Mn）水解所述PEOX。

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