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1. 发明申请

US20220307059A1 METHODS FOR DECOUPLING YIELD AND PRODUCTIVITY OF A NON-CATABOLIC COMPOUND PRODUCED BY A HOST CELL 有权
公开(公告)号：US20220307059A1
公开(公告)日：2022-09-29
申请号：US17616843
申请日：2020-06-05
申请人： AMYRIS, INC.
发明人： Penelope R. CHUA , Joshua Adam LERMAN , Thomas Jon SCHERBART , Chandresh THAKKER , Annie Ening TSONG , Hanxiao JIANG
IPC分类号： C12P5/00 , C12N1/18 , C12N15/81 , C12N9/02 , C12N9/14
摘要： Provided herein are compositions and methods for uncoupling the yield and productivity of an isoprenoid compound produced in a host cell. In some embodiments, the yield and productivity are uncoupled by genetically modifying the host cell to reduce flux through the citric acid cycle (TCA). In other embodiments, the yield and productivity are uncoupled by reducing the levels of ATP in the host cell.

2. 发明申请

US20150093797A1 PRODUCTION OF ACETYL-COENZYME A DERIVED ISOPRENOIDS 审中-公开
标题翻译：乙酰辅酶A衍生的异丙苯的生产
公开(公告)号：US20150093797A1
公开(公告)日：2015-04-02
申请号：US14474976
申请日：2014-09-02
申请人： AMYRIS, INC.
发明人： Timothy Stevens GARDNER , Kristy Michelle HAWKINS , Adam Leon MEADOWS , Annie Ening TSONG , Yoseph TSEGAYE
IPC分类号： C12P5/00 , C12N15/81
CPC分类号： C12P5/007 , C12N9/0006 , C12N9/0008 , C12N9/1288 , C12N15/52 , C12N15/81 , C12P7/42 , C12P23/00 , C12Y101/01034 , C12Y102/0101 , C12Y207/08
摘要： Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.
摘要翻译：本文提供了在宿主细胞中异源产生乙酰辅酶A衍生的类异戊二烯的组合物和方法。在一些实施方案中，宿主细胞被遗传修饰以包含编码乙醛脱氢酶，乙酰化（ADA，E.C.1.2.1.10）和包含使用NADH的HMG-CoA还原酶的MEV途径的异源核苷酸序列。在一些实施方案中，宿主细胞被遗传修饰以包含编码包含乙酰乙酰辅酶A合成酶的ADA和MEV途径的异源核苷酸序列。在一些实施方案中，经遗传修饰的宿主细胞还包含一个或多个编码磷酸酮醇酶和磷酸转乙酰酶的异源核苷酸序列。在一些实施方案中，遗传修饰的宿主细胞还包括天然PDH旁路的功能性破坏。本文描述的组合物和方法提供了用于异源产生乙酰辅酶A衍生的类异戊二烯的能量效率尚未的氧化还原平衡途径。

3. 发明申请

US20130236942A1 PRODUCTION OF ACETYL-COENZYME A DERIVED ISOPRENOIDS 有权
公开(公告)号：US20130236942A1
公开(公告)日：2013-09-12
申请号：US13752293
申请日：2013-01-28
申请人： AMYRIS, INC.
发明人： Timothy Stevens GARDNER , Kristy Michelle HAWKINS , Adam Leon MEADOWS , Annie Ening TSONG , Yoseph TSEGAYE
IPC分类号： C12P5/00 , C12N15/81
CPC分类号： C12P5/007 , C12N9/0006 , C12N9/0008 , C12N9/1288 , C12N15/52 , C12N15/81 , C12P7/42 , C12P23/00 , C12Y101/01034 , C12Y102/0101 , C12Y207/08
摘要： Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.

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