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1. 发明授权

US11940359B2 Preparation of tissue sections using fluorescence-based detection 有权
公开(公告)号：US11940359B2
公开(公告)日：2024-03-26
申请号：US17564447
申请日：2021-12-29
申请人： AGILENT TECHNOLOGIES, INC.
发明人： Kyle Schleifer , Kristin Briana Bernick , Adrienne Mccampbell , Nicholas M. Sampas , Victor Lim
IPC分类号： G01N1/30 , G01N1/06 , G01N1/28 , G01N35/00 , G06T7/00
CPC分类号： G01N1/30 , G01N1/06 , G01N1/286 , G01N35/00594 , G06T7/0012 , G01N2001/068 , G01N2001/2873 , G06T2207/10064 , G06T2207/10152 , G06T2207/30024
摘要： The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or location of the tissue in the embedded sample.

2. 发明申请

US20190194737A1 USE OF TRANSPOSASE AND Y ADAPTERS TO FRAGMENT AND TAG DNA 审中-公开
公开(公告)号：US20190194737A1
公开(公告)日：2019-06-27
申请号：US16295838
申请日：2019-03-07
申请人： AGILENT TECHNOLOGIES, INC.
发明人： Robert A. Ach , Nicholas M. Sampas , Brian Jon Peter
IPC分类号： C12Q1/6855 , C12Q1/6869 , C12Q1/6848 , C12Q1/6874 , C12Q1/6806
CPC分类号： C12Q1/6855 , C12Q1/6806 , C12Q1/6848 , C12Q1/6869 , C12Q1/6874 , C12Q2521/507 , C12Q2565/514
摘要： Described herein is an adapter comprising a population of first oligonucleotides, a second oligonucleotide and a third oligonucleotide, wherein the first oligonucleotides, the second oligonucleotide and the third oligonucleotide are hybridized together to produce a complex that comprises: (i) a first end comprising a transposase recognition sequence, (ii) a central single-stranded region of variable sequence and (iii) a second end comprising sequences that are non-complementary. A method, as well as a kit for practicing the method, are also provided.

3. 发明申请

US20180201924A1 METHOD FOR MAKING AN ASYMMETRICALLY-TAGGED SEQUENCING LIBRARY 审中-公开
公开(公告)号：US20180201924A1
公开(公告)日：2018-07-19
申请号：US15409124
申请日：2017-01-18
申请人： Agilent Technologies, Inc.
发明人： Brian Jon Peter , David Taussig , Bahram Arezi , Robert A. Ach , Nicholas M. Sampas
IPC分类号： C12N15/10 , C12Q1/68
摘要： A method for making an asymmetrically-tagged sequencing library is provided. In some embodiments, the method may comprise: obtaining a symmetrically-tagged library of cDNA or genomic DNA fragments, hybridizing a tailed first primer to the 3′ sequence tag of the library and extending the same to produce primer extension products, and amplifying the primer extension products using a prior of tailed primers to produce asymmetrically-tagged library.

4. 发明申请

US20150361423A1 HIGH THROUGHPUT GENE ASSEMBLY IN DROPLETS 审中-公开
标题翻译：高渗透基因组装在DROPLETS
公开(公告)号：US20150361423A1
公开(公告)日：2015-12-17
申请号：US14486346
申请日：2014-09-15
申请人： Agilent Technologies, Inc.
发明人： Jeffrey R. Sampson , Nicholas M. Sampas
IPC分类号： C12N15/10
CPC分类号： C12N15/1093 , C12N15/1075
摘要： Provided herein is a method comprising: (a) obtaining a mixture of multiple sets of oligonucleotides, wherein the oligonucleotides within each set each comprise a terminal indexer sequence and can be assembled to produce a synthon; and (b) hybridizing the oligonucleotide mixture to an array, thereby spatially-separating the different sets of oligonucleotides from one another. In some embodiments the method may comprise (c) contacting the array with a solution, thereby producing, for each feature bound by the oligonucleotides, a discrete droplet comprising the feature and, optionally, placing an immiscible liquid over the droplets, thereby producing, for each feature bound by the oligonucleotides, a discrete reaction chamber defined by a droplet. The method may further comprise incubating the array under conditions by which a synthon is assembled in each of the reaction chambers. Other embodiments are also provided.
摘要翻译：本文提供的方法包括：（a）获得多组寡核苷酸的混合物，其中每组中的寡核苷酸各自包含末端分子序列，并且可以组装以产生合成子; 和（b）将寡核苷酸混合物与阵列杂交，从而将不同组的寡核苷酸彼此空间分离。在一些实施方案中，该方法可以包括（c）使阵列与溶液接触，从而为由寡核苷酸结合的每个特征产生包含特征的离散液滴，并且任选地将不混溶液体放置在液滴上，从而产生由寡核苷酸结合的每个特征，由液滴限定的离散反应室。该方法还可以包括在每个反应室中组合合成子的条件下孵育该阵列。还提供了其他实施例。

5. 发明申请

US20190195748A1 APPARATUS AND METHODS FOR TRANSFERRING A TISSUE SECTION 审中-公开
公开(公告)号：US20190195748A1
公开(公告)日：2019-06-27
申请号：US16169809
申请日：2018-10-24
申请人： Agilent Technologies, Inc.
发明人： Victor Lim , Richard K. Workman , Nicholas M. Sampas
IPC分类号： G01N1/06 , G01N1/28 , G01N1/31
CPC分类号： G01N1/06 , G01N1/2813 , G01N1/312 , G01N2001/2833
摘要： This invention relates to apparatus and methods for transferring a tissue section to a slide. The invention also relates to automated systems and methods for transferring a tissue section onto a slide using a bath at ambient temperature and for smoothly mounting a tissue section on a slide.

6. 发明申请

US20140212869A1 Nucleic Acid Proximity Assay Involving the Formation of a Three-way junction 审中-公开
标题翻译：涉及形成三向连接点的核酸接近度测定
公开(公告)号：US20140212869A1
公开(公告)日：2014-07-31
申请号：US13750810
申请日：2013-01-25
申请人： AGILENT TECHNOLOGIES, INC.
发明人： Nicholas M. Sampas , Robert A. Ach , Nazumi Alice Yamada , Kristin Bernick
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2522/101 , C12Q2525/161 , C12Q2565/101 , C12Q2525/301 , C12Q2561/125
摘要： Provided herein is a proximity assay that, in certain embodiments, involves: (a) hybridizing a first oligonucleotide and a second oligonucleotide with a target nucleic acid, wherein the first oligonucleotide comprises: i. a region that is complementary to a first sequence in the target nucleic acid and ii. a barcode sequence; and the second oligonucleotide comprises i. a region that is complementary to a second region in the target and ii. the complement of the barcode sequence; and (b) detecting hybridization between the barcode sequence and the complement of the barcode sequence, wherein hybridization between the barcode sequence and the complement of the barcode sequence indicates that the first and second target sequences are proximal to one another in the sample.
摘要翻译：本文提供了在某些实施方案中的邻近测定法，其包括：（a）将第一寡核苷酸和第二寡核苷酸与靶核酸杂交，其中所述第一寡核苷酸包含：与靶核酸中的第一序列互补的区域，和ii。条形码序列; 并且第二寡核苷酸包括i。与目标中的第二区域互补的区域，以及ii。条形码序列的补码; 和（b）检测条形码序列与条形码序列的互补之间的杂交，其中条形码序列与条形码序列的互补之间的杂交表明第一和第二靶序列在样本中彼此靠近。

7. 发明授权

US11535889B2 Use of transposase and Y adapters to fragment and tag DNA 有权
公开(公告)号：US11535889B2
公开(公告)日：2022-12-27
申请号：US16295838
申请日：2019-03-07
申请人： AGILENT TECHNOLOGIES, INC.
发明人： Robert A. Ach , Nicholas M. Sampas , Brian Jon Peter
IPC分类号： C12Q1/6855 , C12Q1/6806 , C12Q1/6848 , C12Q1/6874 , C12Q1/6869
摘要： Described herein is an adapter comprising a population of first oligonucleotides, a second oligonucleotide and a third oligonucleotide, wherein the first oligonucleotides, the second oligonucleotide and the third oligonucleotide are hybridized together to produce a complex that comprises: (i) a first end comprising a transposase recognition sequence, (ii) a central single-stranded region of variable sequence and (iii) a second end comprising sequences that are non-complementary. A method, as well as a kit for practicing the method, are also provided.

8. 发明授权

US11243148B2 Preparation of tissue sections using fluorescence-based detection 有权
公开(公告)号：US11243148B2
公开(公告)日：2022-02-08
申请号：US17129209
申请日：2020-12-21
申请人： AGILENT TECHNOLOGIES, INC.
发明人： Kyle Schleifer , Kristin Briana Bernick , Adrienne Mccampbell , Nicholas M. Sampas , Victor Lim
IPC分类号： G01N1/30 , G01N1/28 , G01N1/06 , G01N35/00 , G06T7/00
摘要： The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or location of the tissue in the embedded sample.

9. 发明申请

US20200264077A1 PREPARATION OF TISSUE SECTIONS USING FLUORESCENCE-BASED DETECTION 审中-公开
公开(公告)号：US20200264077A1
公开(公告)日：2020-08-20
申请号：US16868715
申请日：2020-05-07
申请人： Agilent Technologies, Inc.
发明人： Kyle Schleifer , Kristin Briana Bernick , Adrienne Mccampbell , Nicholas M. Sampas , Victor Lim
IPC分类号： G01N1/30 , G06T7/00 , G01N35/00 , G01N1/06 , G01N1/28
摘要： The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or location of the tissue in the embedded sample.

10. 发明申请

US20190368982A1 PREPARATION OF TISSUE SECTIONS USING FLUORESCENCE-BASED DETECTION 审中-公开
公开(公告)号：US20190368982A1
公开(公告)日：2019-12-05
申请号：US15995755
申请日：2018-06-01
申请人： Agilent Technologies, Inc.
发明人： Kyle Schleifer , Kristin Briana Bernick , Adrienne Mccampbell , Nicholas M. Sampas , Victor Lim
IPC分类号： G01N1/30 , G01N1/28 , G01N1/06 , G01N35/00 , G06T7/00
摘要： The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or location of the tissue in the embedded sample.

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