专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06316238B1 Process for producing activated human ALT 有权
标题翻译：生产活化人类ALT的方法
公开(公告)号：US06316238B1
公开(公告)日：2001-11-13
申请号：US09142481
申请日：1998-09-09
申请人： Atsuo Nakamura , Toshio Tanaka , Yushi Matsuo , Sumio Tanase , Masahiko Funatsu , Akira Eto
发明人： Atsuo Nakamura , Toshio Tanaka , Yushi Matsuo , Sumio Tanase , Masahiko Funatsu , Akira Eto
IPC分类号： C12N910
CPC分类号： C12N9/1096
摘要： The present invention relates to an altered-type human ALT gene in which the codons for the five amino acids in the human ALT (alanine aminotransferase) gene are replaced, i. e. the fourth amino acid codon from the initiation codon for methionine (Met) is replaced by a codon for serine (Ser), the fifth by a codon for threonine (Thr), the seventh by a codon for aspartic acid (Asp), the 39th by a codon for glycine (Gly) and the 222nd by a codon for alanine (Ala), and concurrently, restriction sites are added at the upstream and downstream of said gene. The active human ALT having properties similar to those of the native enzyme can be effectively produced by culturing E. coli transformed with a recombinant plasmid in which the altered-type human ALT gene of the present invention is ligated to a vector.
摘要翻译：本发明涉及一种改变型人类ALT基因，其中人ALT（丙氨酸氨基转移酶）基因中的五个氨基酸的密码子被替换， e。来自甲硫氨酸（Met）的起始密码子的第四个氨基酸密码子被丝氨酸（Ser）的密码子替代，第五个由苏氨酸（Thr）的密码子代替，第七个由天冬氨酸（Asp）的密码子，第39位通过甘氨酸（Gly）的密码子和第222位的丙氨酸（Ala）的密码子，并且同时在所述基因的上游和下游添加限制性位点。具有与天然酶的相似性质的活性人ALT可以通过培养用本发明的改变型人ALT基因连接到载体的重组质粒转化的大肠杆菌有效产生。

2. 发明授权

US5789186A Marker for cerebral apoplexy 失效
标题翻译：脑中风标记
公开(公告)号：US5789186A
公开(公告)日：1998-08-04
申请号：US656934
申请日：1996-06-03
申请人： Hitoshi Takeshita , Hiroshi Morita , Akira Fujiwara , Yasutaka Kaneda , Tohru Watanabe , Taizou Hayashi , Kohji Uchida , Yushi Matsuo , Takekazu Horio
发明人： Hitoshi Takeshita , Hiroshi Morita , Akira Fujiwara , Yasutaka Kaneda , Tohru Watanabe , Taizou Hayashi , Kohji Uchida , Yushi Matsuo , Takekazu Horio
IPC分类号： C12Q1/48 , C12Q1/533 , G01N33/68 , C12Q1/34 , C12Q1/32 , C12Q1/42
CPC分类号： G01N33/6896 , C12Q1/533 , G01N2800/2871
摘要： A sample is treated with a phosphoglyceric acid mutase (PGAM) inhibitor (polythionic acid (salt) such as potassium tetrathionate) to inactivate the M-type isozyme activity and the B-type PGAM is quantified by determining PGAM isozymes by a rate assay. The B-type PGAM is a novel marker for cerebral apoplexy, and the diagnosis of cerebral apoplexy is enabled by assaying it.
摘要翻译：用磷酸甘油酸变位酶（PGAM）抑制剂（多硫酸（盐）如四硫代硫酸钾）处理样品以失活M型同工酶活性，并通过速率测定法测定PGAM同功酶来定量B型PGAM。 B型PGAM是脑中风的新型标志物，通过测定脑卒中的诊断能力。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式