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1. 发明授权

US10234470B2 Enzyme-linked immunosorbent assay kit for detecting dinitolmide and use thereof 有权
公开(公告)号：US10234470B2
公开(公告)日：2019-03-19
申请号：US14412317
申请日：2012-07-04
申请人： Fangyang He , Yuping Wan , Caiwei Feng , Xiaoqin Luo , Jing Feng , Haifeng Cui , Nianqin Xu , Liangliang Zhu , Yumei Liu , Fangfang Jia , Zhengmiao Zhao
发明人： Fangyang He , Yuping Wan , Caiwei Feng , Xiaoqin Luo , Jing Feng , Haifeng Cui , Nianqin Xu , Liangliang Zhu , Yumei Liu , Fangfang Jia , Zhengmiao Zhao
IPC分类号： G01N33/94 , G01N33/577
摘要： The present invention discloses an enzyme-linked immunosorbent assay kit for detecting dinitolmide and use thereof. The enzyme-linked immunosorbent assay kit of the present invention comprises dinitolmide antibody and coating antigen and enzyme conjugate; wherein, the dinitolmide antibody is dinitolmide monoclonal antibody or dinitolmide polyclonal antibody; when the coating antigen is the conjugate of dinitolmide hapten and carrier protein, the enzyme conjugate is enzyme-labeled secondary antibody, or enzyme-labeled specific anti-dinitolmide monoclonal or polyclonal antibody; when the coating antigen is dinitolmide antibody or secondary antibody, the enzyme conjugate is enzyme-labeled dinitolmide hapten. The enzyme-linked immunosorbent assay kit has a simple structure, and it is convenient for use, cheap and portable. Its detection is high effective, accurate, and convenient. It can be used for on-site monitoring and is suitable for qualitative and quantitative screenings of a great number of samples. And thus, it will play an important role in the detection of dinitolmide.

2. 发明申请

US20110111440A1 ELISA KIT FOR DETECTING LINCOMYCIN 有权
标题翻译：用于检测LINCOMYCIN的ELISA试剂盒
公开(公告)号：US20110111440A1
公开(公告)日：2011-05-12
申请号：US12988111
申请日：2008-09-27
申请人： Fangyang He , Yuping Wan , Caiwei Feng , Zhengmiao Zhao , Caimao Feng , Shanliang Wang , Xiaoqin Luo , Xiaobin Ma
发明人： Fangyang He , Yuping Wan , Caiwei Feng , Zhengmiao Zhao , Caimao Feng , Shanliang Wang , Xiaoqin Luo , Xiaobin Ma
IPC分类号： G01N33/53 , C12N5/16 , C07K16/00
CPC分类号： C07K16/1292 , G01N33/9446 , G01N2400/00
摘要： The present invention provides an ELISA kit for detecting lincomycin comprising a coating antigen and an enzyme labeled reagent, wherein the coating antigen is selected from the group consisting of a lincomycin hapten-carrier protein conjugate, a lincomycin antibody and a lincomycin anti-antibody; when the coating antigen is the lincomycin hapten-carrier protein conjugate, the enzyme labeled reagent is an enzyme-labeled lincomycin anti-antibody; when the coating antigen is the lincomycin antibody, the enzyme labeled reagent is an enzyme-labeled lincomycin hapten-carrier protein conjugate; and when the coating antigen is the lincomycin anti-antibody, the enzyme labeled reagent is an enzyme-labeled lincomycin hapten-carrier protein conjugate; and the lincomycin hapten is obtained through the condensation reaction between lincomycin and succinic anhydride. The ELISA kit according to the present invention can be used for detecting the content of lincomycin remained in a sample such as an animal tissue (muscle, liver), honey, etc.
摘要翻译：本发明提供了一种用于检测含有包被抗原和酶标记试剂的林可霉素的ELISA试剂盒，其中所述包被抗原选自林可霉素半抗原 - 载体蛋白缀合物，林可霉素抗体和林可霉素抗体; 当涂层抗原是林可霉素半抗原 - 载体蛋白质缀合物时，酶标记的试剂是酶标记的林可霉素抗体; 当涂层抗原是林可霉素抗体时，酶标记的试剂是酶标记的林可霉素半抗原 - 载体蛋白质缀合物; 当涂层抗原为林可霉素抗体时，酶标记试剂为酶标记的林可霉素半抗原 - 载体蛋白质结合物; 林可霉素半抗原通过林可霉素和琥珀酸酐之间的缩合反应获得。根据本发明的ELISA试剂盒可用于检测留在样品如动物组织（肌肉，肝脏），蜂蜜等中的林可霉素的含量。

3. 发明申请

US20150219678A1 ENZYME-LINKED IMMUNOSORBENT ASSAY KIT FOR DETECTING DINITOLMIDE AND USE THEREOF 审中-公开
标题翻译：用于检测DINITOLMIDE及其使用的酶联免疫测定试剂盒
公开(公告)号：US20150219678A1
公开(公告)日：2015-08-06
申请号：US14412317
申请日：2012-07-04
申请人： Fangyang He , Caiwei Feng , Yuping Wan , Nianqin Xu , Xiaoqin Luo , Jing Feng , Guangcan Tao , Houmei Yu , Yong Li , Sheng Fu , Peng Wu , Haifeng Cui
发明人： Fangyang He , Caiwei Feng , Yuping Wan , Nianqin Xu , Xiaoqin Luo , Jing Feng , Guangcan Tao , Houmei Yu , Yong Li , Sheng Fu , Peng Wu , Haifeng Cui
IPC分类号： G01N33/94 , G01N33/577
CPC分类号： G01N33/94 , G01N33/577 , G01N2430/00
摘要： The present invention discloses an enzyme-linked immunosorbent assay kit for detecting dinitolmide and use thereof. The enzyme-linked immunosorbent assay kit of the present invention comprises dinitolmide antibody and coating antigen and enzyme conjugate; wherein, the dinitolmide antibody is dinitolmide monoclonal antibody or dinitolmide polyclonal antibody; when the coating antigen is the conjugate of dinitolmide hapten and carrier protein, the enzyme conjugate is enzyme-labeled secondary antibody, or enzyme-labeled specific anti-dinitolmide monoclonal or polyclonal antibody; when the coating antigen is dinitolmide antibody or secondary antibody, the enzyme conjugate is enzyme-labeled dinitolmide hapten. The enzyme-linked immunosorbent assay kit has a simple structure, and it is convenient for use, cheap and portable. Its detection is high effective, accurate, and convenient. It can be used for on-site monitoring and is suitable for qualitative and quantitative screenings of a great number of samples. And thus, it will play an important role in the detection of dinitolmide.
摘要翻译：本发明公开了一种用于检测二胺类化合物的酶联免疫吸附测定试剂盒及其应用。本发明的酶联免疫吸附测定试剂盒包含二胺体抗体和包被抗原和酶结合物; 其中，二胺类抗体为二氨基甲酸单克隆抗体或二糖醇多克隆抗体; 当包被抗原是二氨基半胱氨酸半抗原和载体蛋白的结合物时，酶缀合物是酶标记的二抗或酶标记的特异性抗二糖醇单克隆或多克隆抗体; 当包被抗原是二胺抗体或二抗时，酶缀合物是酶标记的二胺体半抗原。酶联免疫吸附测定试剂盒结构简单，使用方便，便宜便携。其检测效率高，准确，方便。它可用于现场监测，适用于大量样品的定性和定量筛选。因此，它将在二硝基苯胺的检测中发挥重要作用。

4. 发明授权

US08507214B2 Elisa kit for detecting lincomycin 有权
标题翻译： Elisa试剂盒检测林可霉素
公开(公告)号：US08507214B2
公开(公告)日：2013-08-13
申请号：US12988111
申请日：2008-09-27
申请人： Fangyang He , Yuping Wan , Caiwei Feng , Zhengmiao Zhao , Caimao Feng , Shanliang Wang , Xiaoqin Luo , Xiaobin Ma
发明人： Fangyang He , Yuping Wan , Caiwei Feng , Zhengmiao Zhao , Caimao Feng , Shanliang Wang , Xiaoqin Luo , Xiaobin Ma
IPC分类号： G01N33/535 , G01N33/53 , C12N5/12 , C07K16/44
CPC分类号： C07K16/1292 , G01N33/9446 , G01N2400/00
摘要： The present invention provides an ELISA kit for detecting lincomycin comprising a coating antigen and an enzyme labeled reagent, wherein the coating antigen is selected from the group consisting of a lincomycin hapten-carrier protein conjugate, a lincomycin antibody and a lincomycin anti-antibody; when the coating antigen is the lincomycin hapten-carrier protein conjugate, the enzyme labeled reagent is an enzyme-labeled lincomycin anti-antibody; when the coating antigen is the lincomycin antibody, the enzyme labeled reagent is an enzyme-labeled lincomycin hapten-carrier protein conjugate; and when the coating antigen is the lincomycin anti-antibody, the enzyme labeled reagent is an enzyme-labeled lincomycin hapten-carrier protein conjugate; and the lincomycin hapten is obtained through the condensation reaction between lincomycin and succinic anhydride. The ELISA kit according to the present invention can be used for detecting the content of lincomycin remained in a sample such as an animal tissue (muscle, liver), honey, etc.
摘要翻译：本发明提供了一种用于检测含有包被抗原和酶标记试剂的林可霉素的ELISA试剂盒，其中所述包被抗原选自林可霉素半抗原 - 载体蛋白缀合物，林可霉素抗体和林可霉素抗体; 当涂层抗原是林可霉素半抗原 - 载体蛋白质缀合物时，酶标记的试剂是酶标记的林可霉素抗体; 当涂层抗原是林可霉素抗体时，酶标记的试剂是酶标记的林可霉素半抗原 - 载体蛋白质缀合物; 当涂层抗原为林可霉素抗体时，酶标记试剂为酶标记的林可霉素半抗原 - 载体蛋白质结合物; 林可霉素半抗原通过林可霉素和琥珀酸酐之间的缩合反应获得。根据本发明的ELISA试剂盒可用于检测留在样品如动物组织（肌肉，肝脏），蜂蜜等中的林可霉素的含量。

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