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1. 发明申请

US20090297657A1 O-acetylhomoserinesulfhydorelace gene and use thereof 审中-公开
标题翻译： O-乙酰高丝氨酸水解酶基因及其用途
公开(公告)号：US20090297657A1
公开(公告)日：2009-12-03
申请号：US11887077
申请日：2006-08-21
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12G1/022 , C07H21/04 , C07K14/39 , C12N15/63 , C12N1/19 , C12Q1/68
CPC分类号： C12C12/006 , C12C12/004 , C12G1/0203 , C12N9/1085
摘要： The present invention relates to a brewery yeast having controlled hydrogen sulfide-producing capability, a process for producing alcoholic beverages with controlled hydrogen sulfide amount. More particularly, the present invention relates to a yeast whose hydrogen sulfide-producing capability that increases the product flavor is controlled by enhancing the expression level of MET17 gene encoding brewery yeast O-acetylhomoserinesulfhydorelace Met17p, particularly non-ScMET17 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.
摘要翻译：本发明涉及一种具有受硫化氢生产能力的啤酒酵母，一种生产具有可控硫化氢含量的酒精饮料的方法。更具体地说，本发明涉及通过提高编码啤酒酵母O-乙酰高丝氨酸亚磺基水合酶Met17p的MET17基因，特别是对大啤酒酵母特异性的非ScMET17基因的表达水平来控制增加产品风味的硫化氢生产能力的酵母，以及用所述酵母生产酒精饮料的方法。

2. 发明申请

US20090269437A1 Glycerol channel gene and use thereof 审中-公开
标题翻译：甘油通道基因及其用途
公开(公告)号：US20090269437A1
公开(公告)日：2009-10-29
申请号：US11990930
申请日：2006-08-31
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12N1/19 , C12N15/11 , C07K14/00 , C12N15/00 , C12Q1/68 , C12C11/09 , C12G1/073 , A23L1/185 , A23L1/202
CPC分类号： C07K14/395 , C12C12/004 , C12C12/006 , C12G1/0203 , C12G3/02
摘要： The present invention relates to a glycerol channel gene and its uses, specifically, a brewery yeast producing alcoholic beverages with excellent body and mellowness, alcoholic beverages produced using the yeast, a process for producing the alcoholic beverages. More particularly, the present invention relates to a yeast whose capability of producing glycerol, which contribute to body and mellowness of products, was controlled by controlling expression level of FPS1 gene encoding brewery yeast glycerol channel Fps1p, particularly non-ScFPS1 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with the yeast.
摘要翻译：本发明涉及一种甘油通道基因及其用途，具体地说，一种具有优异身体和醇厚度的啤酒酵母生产酒精饮料，使用酵母生产的含酒精饮料，生产酒精饮料的方法。更具体地说，本发明涉及通过控制编码啤酒酵母甘油通道Fps1p的FPS1基因的表达水平，特别是对大啤酒特异性的非ScFPS1基因来控制有助于产物的身体和醇味的甘油的生产能力的酵母酵母，以及用酵母生产酒精饮料的方法。

3. 发明申请

US20090246316A1 Esterase gene and use thereof 审中-公开
标题翻译：酯酶基因及其用途
公开(公告)号：US20090246316A1
公开(公告)日：2009-10-01
申请号：US11991065
申请日：2006-09-12
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12Q1/68 , C12N9/16 , C12N15/74 , C12N1/19 , G01N33/569 , C12C11/09 , C12G1/073 , C12Q1/44 , C12Q3/00 , C12R1/645
CPC分类号： C12N9/18 , C12C12/004 , C12C12/006 , C12G1/0203 , C12P7/62
摘要： The present invention relates to a esterase gene and its uses, specifically, a brewery yeast producing alcoholic beverages with excellent aroma and flavor, alcoholic beverages produced using the yeast, a process for producing the alcoholic beverages. More particularly, the present invention relates to a yeast whose capability of producing ester, which contribute to aroma and flavor of products, was controlled by regulating expression level of IAH1 gene encoding brewery yeast esterase Iah1p, particularly nonScIAH1 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with the yeast.
摘要翻译：本发明涉及一种酯酶基因及其用途，具体地说，一种酿造具有优良芳香和风味的酒精饮料的啤酒酵母，使用该酵母生产的含酒精饮料，一种生产该酒精饮料的方法。更具体地，本发明涉及通过调节编码啤酒酵母酯酶Iah1p的IAH1基因，特别是对啤酒酵母特异性的非scIAH1基因的表达水平来控制有助于产物的香味和风味的酵母的酵母，以及涉及一种用酵母生产酒精饮料的方法。

4. 发明申请

US20090175983A1 Gene capable of improving low temperature fermentability and/or low temperature resistance and use thererof 审中-公开
标题翻译：能够改善低温发酵能力和/或耐低温性能的基因
公开(公告)号：US20090175983A1
公开(公告)日：2009-07-09
申请号：US11990942
申请日：2006-08-31
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12C1/00 , C07H21/04 , C07K14/37 , C12N15/63 , C12N1/19 , C12Q1/68 , C12G1/00
CPC分类号： C07K14/395 , C12C11/003 , C12C12/004 , C12C12/006 , C12G1/0203 , C12G2200/11 , C12R1/85
摘要： The present invention relates to a gene capable of improving low temperature performance (low temperature fermentability and/or low temperature resistance) and use thereof, in particular, to a brewery yeast with superior low temperature performance, alcoholic beverages produced using said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose low temperature performance is improved by amplifying expression level of DLT1 gene encoding Dltlp capable of improving low temperature performance of a brewery yeast, especially non-ScDLT1 gene specific to a lager brewing yeast, and to a method for producing alcoholic beverages using said yeast.
摘要翻译：本发明涉及能够改善低温性能（低温发酵性和/或耐低温性）及其使用，特别是具有优异的低温性能的啤酒酵母，使用所述酵母生产的含酒精饮料的基因和生产所述饮料的方法。更具体地说，本发明涉及通过扩增能够提高啤酒酵母的低温性能的特别是非特异性的不含ScDLT1基因的编码Dltlp的DLT1基因的表达水平来提高其低温性能的酵母，以及涉及使用所述酵母生产酒精饮料的方法。

5. 发明申请

US20090117227A1 GENE ENCODING TREHALOSE-6-PHOSPHATE PHOSPHATASE AND USE THEREOF 审中-公开
标题翻译：基因编码TREHALOSE-6磷酸酯磷酸酶及其用途
公开(公告)号：US20090117227A1
公开(公告)日：2009-05-07
申请号：US12279376
申请日：2007-02-01
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12G1/00 , C07H21/04 , C07K14/395 , A23L1/202 , C12Q1/68 , C12N15/63 , C12N1/19
CPC分类号： C12Q1/42 , C12C12/004 , C12C12/006 , C12G1/0203 , C12N9/16 , C12Q1/02 , C12R1/85 , C12Y301/03012 , G01N2333/39
摘要： The present invention relates to a gene encoding trehalose-6-phosphate phosphatase and use thereof, in particular, a yeast for practical use with superior resistance property to dryness and/or low-temperature storage, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose resistance property to dryness and/or resistance property to low-temperature storage is enhanced by amplifying expression level of TPS2 gene encoding a trehalose-6-phosphate phosphatase Tps2p in brewer's yeast, especially non-ScTPS2 gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.
摘要翻译：本发明涉及编码海藻糖-6-磷酸磷酸酶的基因及其用途，特别是用于实际使用的具有优异的耐干性和/或低温储存性的酵母，用所述酵母生产的含酒精饮料的方法和方法用于生产所述饮料。更具体地说，本发明涉及通过扩增酿酒酵母中编码海藻糖-6-磷酸磷酸酶Tps2p的TPS2基因的表达水平，特别是非抑制性酵母，其酵母对抗低温贮藏的耐干性和/ 特异于啤酒酿造酵母的-ScTPS2基因和用酵母等生产酒精饮料的方法

6. 发明申请

US20090053359A1 AMMONIA TRANSPORTER GENE AND USE THEREOF 审中-公开
标题翻译：氨基酸运输基因及其使用
公开(公告)号：US20090053359A1
公开(公告)日：2009-02-26
申请号：US12279080
申请日：2006-12-05
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12G1/00 , C07H21/00 , C07K14/00 , C12N15/00 , C12N15/63 , C12N1/19 , C12Q1/02 , C12Q1/68 , C12C11/00
CPC分类号： C07K14/435 , C12C12/004 , C12C12/006 , C12G1/0203
摘要： The present invention relates to ammonia transporter genes and use thereof. The invention relates in particular to a brewer's yeast which shows enhanced ammonia assimilation, alcoholic beverages produced using such yeast, and a method of producing such alcoholic beverages. More specifically, the invention relates to the MEP3 gene which codes for the ammonia transporter Mep3 in brewer's yeast, particularly to a yeast which can control the ammonia assimilation ability by controlling the level of expression of the nonScMEP3 gene or ScMEP3 gene characteristic to beer yeast and to a method of producing alcoholic beverages using such yeast.
摘要翻译：本发明涉及氨转运蛋白基因及其应用。本发明特别涉及显示增强的氨同化的酿酒酵母，使用这种酵母生产的含酒精饮料，以及生产这种酒精饮料的方法。更具体地，本发明涉及编码啤酒酵母中氨转运蛋白Mep3的MEP3基因，特别是涉及通过控制啤酒酵母特有的nonScMEP3基因或ScMEP3基因的表达水平来控制氨同化能力的酵母，涉及使用这种酵母生产酒精饮料的方法。

7. 发明授权

US5861289A Heat-resistant .beta.-galactosyltransferase, its production process and its use 失效
标题翻译：耐热β-半乳糖转移酶，其生产工艺及其应用
公开(公告)号：US5861289A
公开(公告)日：1999-01-19
申请号：US55553
申请日：1993-05-03
申请人： Toru Nakayama , Yukiko Kodama , Norihide Amano , Masahiro Nakao , Yuji Shibano , Teruo Amachi
发明人： Toru Nakayama , Yukiko Kodama , Norihide Amano , Masahiro Nakao , Yuji Shibano , Teruo Amachi
IPC分类号： A23C9/12 , C12N9/10 , C12P19/18 , C12N9/38 , C12R1/01
CPC分类号： C12Y302/01023 , A23C9/1206 , C12N9/1051 , C12P19/18 , Y10S435/822
摘要： Described herein are a novel heat-resistant .beta.-galactosyltransferase, a production process of the enzyme and a utilization method of the enzyme. The enzyme is produced preferably by produced by a microorganism belonging to the family of Actinomycetaceae, which may be selected from fungi belonging to the genus Saccharopolyspora, the genus Thermomonospora or the genus Thermoactinomvces.
摘要翻译：本文描述了新型耐热β-半乳糖基转移酶，酶的生产方法和酶的利用方法。优选通过由属于放线菌科家族的微生物产生的酶，其可以选自属于Saccharopolyspora属，Thermomonospora属或Thermactinomvces属的真菌。

8. 发明授权

US07893008B2 Oligonucleotides, arrays thereof for detecting microorganisms, and an apparatus, a method and a kit for detecting microorganisms 有权
标题翻译：寡核苷酸，其用于检测微生物的阵列，以及用于检测微生物的装置，方法和试剂盒
公开(公告)号：US07893008B2
公开(公告)日：2011-02-22
申请号：US11990484
申请日：2006-08-16
申请人： Yukiko Kodama , Kazushige Hatanaka , Koichi Tanaka , Hiroko Nakagawa , Shogo Moriya , Kaoru Osano
发明人： Yukiko Kodama , Kazushige Hatanaka , Koichi Tanaka , Hiroko Nakagawa , Shogo Moriya , Kaoru Osano
IPC分类号： C40B30/04 , C40B40/08 , C07H21/04 , C12M1/00 , C12Q1/68
CPC分类号： C12Q1/6895 , C12Q1/6888
摘要： The present invention relates to an instrument, a method and a kit for detecting a microorganism contaminating a subject test sample, which enables one to quickly and accurately identify the microorganism with an easy operation. The instrument for detecting a microorganism according to the present invention relates to a microarray type instrument in which oligonucleotides prepared based on nucleotide sequences specific to the species and genus to which the subject microorganism belongs have been immobilized onto a surface of a substrate. Based on the presence or absence of hybridization of the probes prepared from the test sample with the oligonucleotides immobilized onto the surface of the substrate, the present invention makes it possible to detect and/or identify the microorganism in the test sample easily, quickly and accurately.
摘要翻译：本发明涉及用于检测污染受检试样的微生物的仪器，方法和试剂盒，其能够以简单的操作快速准确地鉴定微生物。根据本发明的用于检测微生物的仪器涉及一种微阵列型仪器，其中基于对象微生物属于的物种和属的特异性的核苷酸序列制备的寡核苷酸已被固定在基底的表面上。基于由测试样品制备的探针与固定在基底表面上的寡核苷酸的杂交的存在或不存在，本发明使得可以容易，快速和准确地检测和/或鉴定测试样品中的微生物。

9. 发明申请

US20090311680A1 Method for Identifying Useful Proteins of Brewery Yeast 审中-公开
标题翻译：识别啤酒酵母有用蛋白质的方法
公开(公告)号：US20090311680A1
公开(公告)日：2009-12-17
申请号：US12224371
申请日：2007-02-26
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga , Takeshi Kanamori
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga , Takeshi Kanamori
IPC分类号： C12Q1/68
CPC分类号： C12N15/1034
摘要： The invention relates to a method for identifying a useful protein of brewery yeast. More specifically, the invention relates to (a) cultivating yeast under a predetermined cultivation condition; (b) extracting a protein sample from the cultivation product of the yeast; (c) separating the protein sample by a protein separation means, selecting a target peak or spot, and recovering the target protein or a fragment thereof contained in the peak or spot; (d) determining the amino acid sequence of the target protein; (e) comparing the amino acid sequence determined in step (d) with the amino acid sequence determined in advance based on all or a part of genome sequence information of bottom fermenting yeast; (f) identifying the target protein and the gene encoding the target protein based on the results of comparison; and (g) analyzing functions of the identified gene to identify characters given to the yeast by the gene.
摘要翻译：本发明涉及鉴定啤酒酵母有用蛋白质的方法。更具体地，本发明涉及（a）在预定的培养条件下培养酵母; （b）从酵母的培养产物中提取蛋白质样品; （c）通过蛋白质分离装置分离蛋白质样品，选择目标峰或斑点，并回收峰或点中所含的靶蛋白或其片段; （d）确定靶蛋白的氨基酸序列; （e）基于底部发酵酵母的全部或部分基因组序列信息，比较步骤（d）中确定的氨基酸序列与预先确定的氨基酸序列; （f）基于比较结果鉴定靶蛋白和编码靶蛋白的基因; 和（g）分析所鉴定的基因的功能以鉴定由该基因给予酵母的字符。

10. 发明申请

US20090274794A1 Gene Encoding Cell Wall Mannoprotein and use Thererof 审中-公开
标题翻译：基因编码细胞壁肌肉蛋白并使用其中
公开(公告)号：US20090274794A1
公开(公告)日：2009-11-05
申请号：US11991797
申请日：2006-09-21
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga , Fumihiko Ohmura
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga , Fumihiko Ohmura
IPC分类号： C12N1/19 , C07H21/04 , C07K14/00 , C12N15/63 , C12Q1/68 , C12C11/09 , A23L1/185 , C12G1/073 , C12H1/14
CPC分类号： C12C12/004 , C07K14/395 , C12C12/006 , C12G1/0203 , C12G3/02
摘要： The present invention relates to a brewer's yeast which produces alcoholic beverages having an ability for reducing the haze level, alcoholic beverages produced using such a yeast, and a method of producing such alcoholic beverages. More specifically, the present invention relates to a yeast which can reduce the level of haze in the product by increasing the level of expression of ScCWP2 gene encoding cell wall mannoprotein Cwp2p in brewer's yeast, or non-ScCWP2 gene characteristic to beer yeast, and to a method of producing alcoholic beverages using such a yeast.
摘要翻译：本发明涉及生产具有降低雾度的能力的酒精饮料的啤酒酵母，使用这种酵母生产的含酒精饮料，以及这种酒精饮料的制造方法。更具体地说，本发明涉及通过提高酿酒酵母中编码细胞壁甘露糖蛋白Cwp2p的ScCWP2基因或啤酒酵母特有的非ScCWP2基因的表达水平，能够降低产品的雾度的酵母，使用这样的酵母生产酒精饮料的方法。

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