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1. 发明申请

US20110003320A1 IMMUNOASSAY METHOD AND KIT AND DEVELOPING SOLVENT THEREFOR 审中-公开
标题翻译：免疫方法和套件及其开发溶剂
公开(公告)号：US20110003320A1
公开(公告)日：2011-01-06
申请号：US12921356
申请日：2009-02-27
申请人： Daisuke Ito , Hisahiko Iwamoto , Kazuyoshi Mochiduki , Riku Tsuge , Yoshiko Kitani
发明人： Daisuke Ito , Hisahiko Iwamoto , Kazuyoshi Mochiduki , Riku Tsuge , Yoshiko Kitani
IPC分类号： G01N33/566
CPC分类号： G01N33/54393 , G01N33/558 , G01N2400/00
摘要： An immunoassay method and a kit which comprises a combination of a test piece for immunochromatography and a developing solvent, by which a target substance is detected accurately in a short period of time while a preventing a nonspecific reaction.The above method is to immunologically measure the target substance by using the test piece for immunochromatography which comprises a membrane carrier having a first antibody or a first antigen capable of binding specifically to the target substance immobilized thereon, an insoluble carrier having a second antibody or a second antigen capable of binding specifically to the target substance immobilized thereon and a porous separation matrix capable of cell separation, wherein the porous separation matrix is impregnated with a surfactant in advance, and the insoluble carrier bound specifically to the target substance through the second antibody or the second antigen is developed with a developing solvent containing mannitol.
摘要翻译：免疫测定方法和试剂盒，其包含用于免疫色谱的测试片和显影溶剂的组合，通过该试剂在短时间内准确检测到目标物质，同时防止非特异性反应。上述方法是通过使用免疫色谱法的试验片免疫测定目标物质，其包括具有第一抗体或能够特异性结合固定于其上的目标物质的第一抗原的膜载体，具有第二抗体或能够特异性结合固定于其上的靶物质的第二抗原和能够进行细胞分离的多孔分离基质，其中多孔分离基质预先用表面活性剂浸渍，并且不溶性载体通过第二抗体特异性结合靶物质，或第二抗原用含甘露醇的显影溶剂显影。

2. 发明授权

US09360431B2 Blue-colored gold nanoparticles for immunological measurement, process for production of same, and measurement method using same 有权
标题翻译：用于免疫测量的蓝色金纳米颗粒，其制备方法和使用其的测量方法
公开(公告)号：US09360431B2
公开(公告)日：2016-06-07
申请号：US13883213
申请日：2011-11-04
申请人： Yuya Kato , Daisuke Ito , Yoshiko Kitani
发明人： Yuya Kato , Daisuke Ito , Yoshiko Kitani
IPC分类号： G01N33/543 , G01N21/75 , G01N33/58 , B22F1/00 , B22F9/24 , B82Y30/00
CPC分类号： G01N21/75 , B22F1/0025 , B22F9/24 , B22F2301/255 , B22F2304/054 , B22F2304/056 , B82Y30/00 , G01N33/585
摘要： Gold nanoparticles which comprises organic buffer containing a piperazine ring, gold, and an organic acid having reducing properties and which shows a blue color by visually view when it is dispersed as a colloidal solution, can be produced easily by conducting a nucleus formation step by reacting organic acid containing a piperazine ring with a solution of a first gold salt to form nucleus gold nanoparticles and a growth step by simultaneously adding and reacting a solution of a second gold salt and an organic acid having reducing properties with a solution of the nucleus gold nanoparticle to grow the nucleus gold nanoparticles. The produced gold nanoparticles can be used as labeling particles in an immunological measurement method.
摘要翻译：包含具有还原性的有机缓冲剂的金纳米粒子，金和具有还原性的有机酸，当作为胶体溶液分散时通过视觉观察显示蓝色的金纳米颗粒可以容易地通过进行核形成步骤来制备，含有第一金盐溶液形成核金纳米颗粒的哌嗪环的有机酸和生长步骤，通过同时加入并使具有还原性的有机酸溶液与核金纳米颗粒的溶液反应生长核金纳米颗粒。所制备的金纳米颗粒可用作免疫测量方法中的标记颗粒。

3. 发明申请

US20130224885A1 BLUE-COLORED GOLD NANOPARTICLES FOR IMMUNOLOGICAL MEASUREMENT, PROCESS FOR PRODUCTION OF SAME, AND MEASUREMENT METHOD USING SAME 有权
标题翻译：用于免疫测量的蓝色金纳米颗粒，其生产方法和使用其的测量方法
公开(公告)号：US20130224885A1
公开(公告)日：2013-08-29
申请号：US13883213
申请日：2011-11-04
申请人： Yuya Kato , Daisuke Ito , Yoshiko Kitani
发明人： Yuya Kato , Daisuke Ito , Yoshiko Kitani
IPC分类号： G01N21/75
CPC分类号： G01N21/75 , B22F1/0025 , B22F9/24 , B22F2301/255 , B22F2304/054 , B22F2304/056 , B82Y30/00 , G01N33/585
摘要： Gold nanoparticles which comprises organic buffer containing a piperazine ring, gold, and an organic acid having reducing properties and which shows a blue color by visually view when it is dispersed as a colloidal solution, can be produced easily by conducting a nucleus formation step by reacting organic acid containing a piperazine ring with a solution of a first gold salt to form nucleus gold nanoparticles and a growth step by simultaneously adding and reacting a solution of a second gold salt and an organic acid having reducing properties with a solution of the nucleus gold nanoparticle to grow the nucleus gold nanoparticles. The produced gold nanoparticles can be used as labeling particles in an immunological measurement method.
摘要翻译：包含具有还原性的有机缓冲剂的金纳米粒子，金和具有还原性的有机酸，当作为胶体溶液分散时通过视觉观察显示蓝色的金纳米颗粒可以容易地通过进行核形成步骤来制备，含有第一金盐溶液形成核金纳米颗粒的哌嗪环的有机酸和生长步骤，通过同时加入并使具有还原性的有机酸溶液与核金纳米颗粒的溶液反应生长核金纳米颗粒。所制备的金纳米颗粒可用作免疫测量方法中的标记颗粒。

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