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    • 3. 发明授权
    • Method of preparing live viral vaccines by genetic engineering of viral genome
    • 通过病毒基因组的遗传工程制备活病毒疫苗的方法
    • US08834892B2
    • 2014-09-16
    • US13214634
    • 2011-08-22
    • Baik Lin SeongYo Han JangKwang Hee LeeYoung Ho Byun
    • Baik Lin SeongYo Han JangKwang Hee LeeYoung Ho Byun
    • A61K39/145A61K39/12C12N7/00
    • A61K39/145A61K39/12A61K2039/5254A61K2039/543C07K2319/50C12N7/00C12N2760/16122C12N2760/16134C12N2760/16162Y02A50/388
    • Disclosed is an attenuation method of an influenza virus, that is, a reassortant virus and a preparation method thereof. The disclosed reassortant virus has, in a ratio of 6:2, genes encoding a recombinant non-toxic protein and a wild type non-toxic protein, and genes encoding toxic proteins, HA (hemagglutinin) and NA (neuraminidase), of an influenza virus, the recombinant non-toxic protein consisting of a substituted caspase recognition sequence without a change of a protein size within the wild type non-toxic protein of the influenza virus. The disclosed attenuated influenza virus shows a high attenuation without a reduction of productivity in a fertilized egg. Accordingly, the method can be used as an economically efficient live vaccine preparation method which has both safety and efficiency and can use a fertilized egg as a production system. Also, since a protein is not removed or modified during attenuation, the method can be used in combination with a conventional attenuated vaccine preparation technology such as cold-adaptation.
    • 公开了流感病毒的减毒方法,即重配病毒及其制备方法。 公开的重配病毒以6:2的比例存在编码重组无毒蛋白质和野生型无毒蛋白质的基因,以及编码流感的毒性蛋白质HA(血凝素)和NA(神经氨酸酶)的基因) 病毒,由流感病毒的野生型无毒蛋白质中没有蛋白质大小变化的取代的半胱天冬酶识别序列组成的重组无毒蛋白质。 所公开的减毒流感病毒显示出高的衰减,而不会降低受精卵的生产力。 因此,该方法可以用作经济有效的活疫苗制备方法,其具有安全性和效率,并且可以使用受精卵作为生产系统。 此外,由于蛋白质在衰减期间不被去除或修饰,所以该方法可以与传统的减毒疫苗制备技术如冷适应联合使用。