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1. 发明申请

US20070141698A1 Microbial protein expression system utilizing plant viral coat protein 审中-公开
标题翻译：利用植物病毒外壳蛋白的微生物蛋白表达系统
公开(公告)号：US20070141698A1
公开(公告)日：2007-06-21
申请号：US11311197
申请日：2005-12-19
申请人： Chung-Chi Hu , Chia-Ying Wu , Yi-Chin Lai , Hsin-Tsu Tsai , Hsin-Huang Chen
发明人： Chung-Chi Hu , Chia-Ying Wu , Yi-Chin Lai , Hsin-Tsu Tsai , Hsin-Huang Chen
IPC分类号： C12P21/06 , C12N15/00
CPC分类号： C12N15/62 , C07K2319/35 , C12N15/70 , C12P21/02
摘要： The present invention relates to an efficient microbial protein production system. A target protein is expressed as a portion of a fusion protein with the coat protein of Cymbidium mosaic virus (CyMV) in E. coli. Accordingly, the present invention provides nucleic acid expression vectors comprising a sequence encoding a protein of interest fused to CyMV coat protein, as well as methods of utilizing such vectors to produce the protein of interest.
摘要翻译：本发明涉及有效的微生物蛋白质生产系统。靶蛋白在大肠杆菌中表达为与蕙兰花叶病毒（CyMV）的外壳蛋白的融合蛋白的一部分。因此，本发明提供了包含编码与CyMV外壳蛋白融合的感兴趣的蛋白质的序列的核酸表达载体，以及利用这些载体产生感兴趣的蛋白质的方法。

2. 发明申请

US20110053258A1 Novel promoter sequence and the application thereof 审中-公开
标题翻译：新型启动子序列及其应用
公开(公告)号：US20110053258A1
公开(公告)日：2011-03-03
申请号：US12155393
申请日：2008-06-03
申请人： Chung-Chi Hu , Wei-Chen Wang , Chia-Ying Wu , Yau-Heiu Hsu , Yi-Chin Lai
发明人： Chung-Chi Hu , Wei-Chen Wang , Chia-Ying Wu , Yau-Heiu Hsu , Yi-Chin Lai
IPC分类号： C12N15/63 , C07H21/00
CPC分类号： C12N15/85
摘要： The present invention provides a novel promoter sequence derived from a promoter of geminivirus, a eukaryotic virus, and has the characteristics of prokaryotic promoters. The isolated sequence includes SEQ ID NO: 5, which can drive the expression of foreign genes in prokaryotic cells to a high level utilizing the prokaryotic RNA polymerases. The present invention is a constitutive promoter which does not require the addition of external inducers to promote high-level gene expressions. The promoter activity of the present invention is 15-fold higher than the Rep gene promoter of geminivirus, which is also active in prokaryotic cells. Compared to the promoter activity of the standard constitutive prokaryotic promoter rrnB P1, the activity of the present invention is 11.1% higher. The activity of the present invention is additive when concatenated in the same polarity in the constructs, further enhancing the expression level of genes.
摘要翻译：本发明提供了源自双子a病毒真核病毒启动子的新型启动子序列，具有原核启动子的特征。分离的序列包括SEQ ID NO：5，其可以利用原核RNA聚合酶将原核细胞中的外源基因的表达驱动到高水平。本发明是一种组成型启动子，其不需要添加外部诱导物来促进高水平的基因表达。本发明的启动子活性比双原子病毒的Rep基因启动子高15倍，这也是在原核细胞中也有活性的。与标准组成型原核启动子rrnB P1的启动子活性相比，本发明的活性高11.1％。当在构建体中以相同的极性连接时，本发明的活性是加成的，进一步提高了基因的表达水平。

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