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1. 发明授权

US06777234B1 Mammalian muscle construct and method for producing same 失效
标题翻译：哺乳动物肌肉构造及其生产方法
公开(公告)号：US06777234B1
公开(公告)日：2004-08-17
申请号：US09709890
申请日：2000-11-09
申请人： Robert G. Dennis , Paul Kosnik , William M. Kuzon, Jr. , John A. Faulkner
发明人： Robert G. Dennis , Paul Kosnik , William M. Kuzon, Jr. , John A. Faulkner
IPC分类号： C12N500
CPC分类号： C12N5/0658 , A61K35/12 , C12N2533/52
摘要： A mammalian muscle construct and a method for producing the construct are provided. The mammalian muscle construct includes a substrate and a plurality of separate anchors secured to the substrate. Myogenic precursor cells are provided on the substrate with at least some of the cells in contact with the anchors. The myogenic precursor cells are cultured in vitro under conditions to allow the cells to become confluent between the anchors. The anchors are receptive to the cells and allow the cells to attach thereto, such that placement of the anchors controls the size and shape of the muscle construct formed. Specifically, the anchors include separate fragments of biocompatible material secured to the substrate, wherein cell adhesion molecules are associated with each fragment to facilitate attachment of the precursor cells to the fragment.
摘要翻译：提供哺乳动物肌肉构造物和制备该构建体的方法。哺乳动物肌肉构造物包括基底和固定到基底的多个单独的锚。在底物上提供生肌前体细胞，其中至少一些细胞与锚固体接触。在体外培养肌原性前体细胞的条件下使细胞在锚定体之间变得融合。锚固体接受细胞并允许细胞附着于其上，使得锚的放置控制形成的肌肉构造的大小和形状。具体地，锚固体包括固定到底物的生物相容性材料的分开的片段，其中细胞粘附分子与每个片段相关联，以促进前体细胞与片段的连接。

2. 发明授权

US06448076B2 Method for chemically acellularizing a biological tissue sample 有权
标题翻译：化学脱细胞化生物组织样品的方法
公开(公告)号：US06448076B2
公开(公告)日：2002-09-10
申请号：US09896651
申请日：2001-06-29
申请人： Robert G. Dennis , William M. Kuzon, Jr. , Paul S. Cederna
发明人： Robert G. Dennis , William M. Kuzon, Jr. , Paul S. Cederna
IPC分类号： C12N500
CPC分类号： C12N5/0658 , A61K35/12 , C12N2533/52 , C12N2533/92
摘要： A method for chemically acellularizing a biological tissue sample, such as a peripheral nerve, is provided. The method includes disrupting the cell membranes of the biological tissue sample, and then denaturing intracellular proteins within the cells of the tissue sample and removing the denatured proteins from the cells while preserving the extracellular matrix to produce an acellularized tissue construct.
摘要翻译：提供了一种对生物组织样品如周围神经进行化学脱细胞化的方法。该方法包括破坏生物组织样品的细胞膜，然后使组织样品的细胞内的细胞内蛋白质变性并从细胞中除去变性的蛋白质，同时保留细胞外基质以产生无细胞化的组织构建体。

3. 发明授权

US06207451B1 Mammalian muscle construct and method for producing same 失效
标题翻译：哺乳动物肌肉构造及其生产方法
公开(公告)号：US06207451B1
公开(公告)日：2001-03-27
申请号：US09153721
申请日：1998-09-15
申请人： Robert G. Dennis , Paul Kosnik , William M. Kuzon, Jr. , John A. Faulkner
发明人： Robert G. Dennis , Paul Kosnik , William M. Kuzon, Jr. , John A. Faulkner
IPC分类号： C12N500
CPC分类号： C12N5/0658 , A61K35/12 , C12N2533/52
摘要： A mammalian muscle construct and a method for producing the construct are provided. The mammalian muscle construct includes a substrate and a plurality of separate anchors secured to the substrate. Myogenic precursor cells are provided on the substrate with at least some of the cells in contact with the anchors. The myogenic precursor cells are cultured in vitro under conditions to allow the cells to become confluent between the anchors. The anchors are receptive to the cells and allow the cells to attach thereto, such that placement of the anchors controls the size and shape of the muscle construct formed. Specifically, the anchors include separate fragments of biocompatible material secured to the substrate, wherein cell adhesion molecules are associated with each fragment to facilitate attachment of the precursor cells to the fragment.
摘要翻译：提供哺乳动物肌肉构造物和制备该构建体的方法。哺乳动物肌肉构造物包括基底和固定到基底的多个单独的锚。在底物上提供生肌前体细胞，其中至少一些细胞与锚固体接触。在体外培养肌原性前体细胞的条件下使细胞在锚定体之间变得融合。锚固体接受细胞并允许细胞附着于其上，使得锚的放置控制形成的肌肉构造的大小和形状。具体地，锚固体包括固定到底物的生物相容性材料的分开的片段，其中细胞粘附分子与每个片段相关联，以促进前体细胞与片段的连接。

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