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1. 发明申请

US20120028358A1 METHOD FOR INCREASING RETROVIRAL INFECTIVITY 审中-公开
标题翻译：增加退行性感染的方法
公开(公告)号：US20120028358A1
公开(公告)日：2012-02-02
申请号：US13170836
申请日：2011-06-28
申请人： Victor Solodushko , Brian Fouty
发明人： Victor Solodushko , Brian Fouty
IPC分类号： C12N15/867 , C12N5/071
CPC分类号： C12N15/86 , C12N2740/10043 , C12N2740/10051 , C12N2740/13043 , C12N2740/13051
摘要： The present invention is directed to methods for enhanced retroviral delivery of a nucleic acid to a target cell in vitro, ex vivo or in vivo, which involves increasing infectivity of a retrovirus carrying a transgene of interest to a target cell. In one particular aspect the invention relates to a method for increasing retroviral infectivity of target cells comprising culturing packaging cells transfected with retroviral vector containing a transgene of interest in medium containing a glucocorticoid receptor agonist or analog or derivative thereof present in the medium in an amount effective to increase titer of propagated retrovirus; and subsequently culturing a target cell in medium comprising the propagated retrovirus from a) and a glucocorticoid receptor antagonist or analog or derivative thereof present in the medium in an amount effective to increase target cell sensitivity to infection by the propagated retrovirus.
摘要翻译：本发明涉及用于在体外，体外或体内增强核酸向靶细胞的逆转录病毒递送的方法，其涉及将携带感兴趣的转基因的逆转录病毒的感染性增加至靶细胞。在一个特定方面，本发明涉及一种用于增加靶细胞的逆转录病毒感染性的方法，所述方法包括用存在于培养基中的培养含有目的转基因的逆转录病毒载体转染培养包含细胞的培养基，所述培养基含有存在于培养基中的糖皮质激素受体激动剂或其类似物或衍生物增加传播逆转录病毒的滴度; 并且随后在来自a）的培养基中培养包含增殖的逆转录病毒的靶细胞，并且以有效增加靶细胞对传播的逆转录病毒感染的敏感性的量存在于培养基中的糖皮质激素受体拮抗剂或其类似物或衍生物。

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