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1. 发明申请

US20050032088A1 2,5-DKG permeases 有权
标题翻译： 2,5-DKG透气
公开(公告)号：US20050032088A1
公开(公告)日：2005-02-10
申请号：US10787267
申请日：2004-02-25
申请人： Veronique Dartois , James Hoch , Fernando Valle , Manoj Kumar
发明人： Veronique Dartois , James Hoch , Fernando Valle , Manoj Kumar
IPC分类号： C07K14/26 , C12N15/54 , C12Q1/68 , C07H21/04 , C12N9/10 , C12P7/60
CPC分类号： C07K14/26
摘要： The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease activity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.
摘要翻译：本发明提供了编码具有2,5-DKG通透酶活性的多肽的分离的核酸分子及其寡核苷酸。分离的核酸分子可以在适当的细菌细胞中表达以增强2-KLG的产生，随后将其转化为抗坏血酸。还提供了具有2,5-DKG通透酶活性的分离的多肽，其免疫原性肽及其特异的抗体。本发明还提供鉴定新型2,5-DKG通透酶的方法。

2. 发明申请

US20070298474A1 Enhanced 2-Keto-L-Gulonic acid production 审中-公开
标题翻译：增强的2-酮-L-古洛糖酸生产
公开(公告)号：US20070298474A1
公开(公告)日：2007-12-27
申请号：US11879260
申请日：2007-07-16
申请人： Manoj Kumar , Fernando Valle , Veronique Dartois , James Hoch
发明人： Manoj Kumar , Fernando Valle , Veronique Dartois , James Hoch
IPC分类号： C12P7/02
CPC分类号： C12P7/60
摘要： A method for enhancing a host cell's biosynthetic production of 2-KLG is described. Such method comprises selecting a host cell that has an at least partially intracellular synthetic pathway which utilizes 2,5-DKG to produce 2-KLG; increasing the transport of said 2,5-DKG into said host cell while maintaining the integrity of the host cell; culturing the host cell to produce said 2,5-DKG; and producing 2-KLG. The transport of the 2,5-DKG is increased by transforming into the host cell DNA encoding for one or more enzymes transporting the 2,5-DKG into the host cell.
摘要翻译：描述了增强宿主细胞2-KLG生物合成生产的方法。这种方法包括选择具有至少部分细胞内合成途径的宿主细胞，其利用2,5-DKG产生2-KLG; 增加所述2,5-DKG进入所述宿主细胞的同时保持宿主细胞的完整性; 培养宿主细胞以产生所述2,5-DKG; 并生产2-KLG。通过将编码一种或多种将2,5-DKG转运到宿主细胞的酶的宿主细胞DNA转化成2,5-DKG的转运来增加。

3. 发明申请

US20060147950A1 Precursor conversion screening method 审中-公开
标题翻译：前体转化筛选方法
公开(公告)号：US20060147950A1
公开(公告)日：2006-07-06
申请号：US11223258
申请日：2005-09-09
申请人： James Hoch , Veronique Dartois
发明人： James Hoch , Veronique Dartois
IPC分类号： C40B40/08
CPC分类号： G01N33/502 , C12N15/1034 , C12Q1/18 , G01N33/5008 , G01N33/5011 , G01N33/5014 , G01N33/5038
摘要： The invention provides methods of identifying a host cell that encodes a metabolic pathway that converts a precursor molecule into a growth inhibitory compound, by: (a) culturing a population of host cells under conditions that allow expression of the metabolic pathway; (b) contacting the host cells, or an extract thereof, with a population of target cells and the precursor molecule; and (c) identifying a host cell that inhibits growth of the target cells in the presence, but not in the absence, of the precursor molecule, where an identified host cell from step (c) contains a metabolic pathway that converts the precursor molecule into a growth inhibitory compound. Methods of identifying host cells that encode a metabolic pathway that converts a precursor molecule into a desired product compound are also provided. Further. provided are methods of identifying nucleic acid molecules encoding the metabolic pathways.
摘要翻译：本发明提供了鉴定编码代谢途径的宿主细胞的方法，所述代谢途径通过以下方式将前体分子转化成生长抑制化合物：（a）在允许代谢途径表达的条件下培养宿主细胞群; （b）使宿主细胞或其提取物与靶细胞群和前体分子接触; （c）鉴定在前体分子的存在但不存在的情况下抑制靶细胞生长的宿主细胞，其中来自步骤（c）的鉴定的宿主细胞含有将前体分子转化为生长抑制化合物。还提供了鉴定编码将前体分子转化为所需产物化合物的代谢途径的宿主细胞的方法。进一步。提供了鉴定编码代谢途径的核酸分子的方法。

4. 发明授权

US06368793B1 Metabolic selection methods 失效
标题翻译：代谢选择方法
公开(公告)号：US06368793B1
公开(公告)日：2002-04-09
申请号：US09172952
申请日：1998-10-14
申请人： James Hoch , Veronique Dartois
发明人： James Hoch , Veronique Dartois
IPC分类号： C12Q168
CPC分类号： C12N15/52 , C12Q1/6897
摘要： The present invention relates in part to methods for screening for novel enzymatic pathways in environmental samples using metabolic selection strategies, and the isolation of the genes and proteins that make up these pathways.
摘要翻译：本发明部分涉及使用代谢选择策略筛选环境样品中新的酶途径的方法，以及构成这些途径的基因和蛋白质的分离。

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