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1. 发明申请

US20060148048A1 Process for acyl-transfer enzyme reactions with acyl-coenzyme a 有权
标题翻译：用酰基辅酶a进行酰基转移酶反应的方法
公开(公告)号：US20060148048A1
公开(公告)日：2006-07-06
申请号：US10542733
申请日：2004-01-21
申请人： Motoaki Kamachi , Harumi Kamachi , Hirobumi Aoki , Tomoki Erata , Kenji Tajima
发明人： Motoaki Kamachi , Harumi Kamachi , Hirobumi Aoki , Tomoki Erata , Kenji Tajima
IPC分类号： C12P7/64 , C12P7/62
CPC分类号： C12P13/02 , C12N9/1029 , C12P7/625 , C12P13/001
摘要： The present invention relates to a method for acyltransferase reaction in which an acyl group of acyl coenzyme A is transferred to an acyl group receptor characterized in that the reaction is carried out by production and/or reproduction of acyl coenzyme A from coenzyme A in a reaction system by a chemical thioester exchange reaction with acylthioester. The present invention, wherein expensive acyl CoA is reproduced nonenzymatically in a reaction system, enables to continuously carry out acyltransferase reaction only by putting a small amount of acyl CoA with a donor and a receptor of an acyl group into a system. Accordingly, the method of the present invention can be applied to an industrial production method of various kinds of compounds including useful biological molecules and synthesis of polylmers such as polyester.
摘要翻译：本发明涉及一种酰基转移酶反应的方法，其中酰基辅酶A的酰基被转移到酰基基团受体中，其特征在于反应是通过在反应中由辅酶A生产和/或再生酰基辅酶A 系统通过化学硫酯与酰基硫酯交换反应。其中昂贵的酰基CoA在反应体系中非酶原地再生的本发明使得能够仅通过将少量酰基辅酶A与酰基的供体和受体一起投入系统而连续进行酰基转移酶反应。因此，本发明的方法可以应用于包括有用的生物分子和聚合物如聚酯的合成的各种化合物的工业生产方法。

2. 发明授权

US07943351B2 Method for acyltransferase reaction using acyl coenzyme A 有权
标题翻译：使用酰基辅酶A进行酰基转移酶反应的方法
公开(公告)号：US07943351B2
公开(公告)日：2011-05-17
申请号：US12277622
申请日：2008-11-25
申请人： Motoaki Kamachi , Harumi Kamachi , Hirobumi Aoki , Tomoki Erata , Kenji Tajima
发明人： Motoaki Kamachi , Harumi Kamachi , Hirobumi Aoki , Tomoki Erata , Kenji Tajima
IPC分类号： C12P11/00 , C12P7/64 , C12P7/62
CPC分类号： C12P13/02 , C12N9/1029 , C12P7/625 , C12P13/001
摘要： The present invention relates to a method for acyltransferase reaction in which an acyl group of acyl coenzyme A is transferred to an acyl group receptor characterized in that the reaction is carried out by production and/or reproduction of acyl coenzyme A from coenzyme A in a reaction system by a chemical thioester exchange reaction with acylthioester. The present invention, wherein expensive acyl CoA is reproduced nonenzymatically in a reaction system, enables to continuously carry out acyltransferase reaction only by putting a small amount of acyl CoA with a donor and a receptor of an acyl group into a system. Accordingly, the method of the present invention can be applied to an industrial production method of various kinds of compounds including useful biological molecules and synthesis of polymers such as polyester.
摘要翻译：本发明涉及一种酰基转移酶反应的方法，其中酰基辅酶A的酰基被转移到酰基基团受体中，其特征在于反应是通过在反应中由辅酶A生产和/或再生酰基辅酶A 系统通过化学硫酯与酰基硫酯交换反应。其中昂贵的酰基CoA在反应体系中非酶原地再生的本发明使得能够仅通过将少量酰基辅酶A与酰基的供体和受体一起投入系统而连续进行酰基转移酶反应。因此，本发明的方法可以应用于包括有用的生物分子和聚合物如聚酯的合成的各种化合物的工业生产方法。

3. 发明申请

US20050214374A1 Artificial extracellular matrix and process for producing the same 审中-公开
标题翻译：人造细胞外基质及其制备方法
公开(公告)号：US20050214374A1
公开(公告)日：2005-09-29
申请号：US10514252
申请日：2002-05-14
申请人： Mitsuo Takai , Tomoki Erata , Shunji Yunoki , Tatsuya Fujita
发明人： Mitsuo Takai , Tomoki Erata , Shunji Yunoki , Tatsuya Fujita
IPC分类号： A61K38/39 , A61L27/24 , A61L27/38 , A61L27/56 , A61L27/60 , A61K38/17 , A61K9/14
CPC分类号： A61K38/39 , A61L27/24 , A61L27/38 , A61L27/56 , A61L27/60
摘要： The artificial extracellular matrix of the present invention comprises heat stable fish collagen produced from fish collagen with a heat denaturation temperature lower than 37° C. by improving its heat stability. The artificial extracellular matrix of the invention has an adequate stability in living bodies and a function to activate tissue repair at a high level and requires no addition of heat denatured collagen. In addition, the artificial extracellular matrix is unlikely to contain pathogenic organisms because it uses collagen originated from fish.
摘要翻译：本发明的人造细胞外基质包括通过改善其热稳定性，通过热变性温度低于37℃的由鱼胶原产生的热稳定鱼胶原蛋白。本发明的人造细胞外基质在活体中具有足够的稳定性，并且具有高水平激活组织修复的功能，并且不需要添加热变性胶原蛋白。此外，人造细胞外基质不可能含有致病生物，因为它使用源于鱼的胶原蛋白。

4. 发明申请

US20090111153A1 METHOD FOR ACYLTRANSFERASE REACTION USING ACYL COENZYME A 有权
标题翻译：使用ACYL COENZYME的ACYLTRANSFERASE反应的方法A
公开(公告)号：US20090111153A1
公开(公告)日：2009-04-30
申请号：US12277622
申请日：2008-11-25
申请人： Motoaki KAMACHI , Harumi Kamachi , Hirobumi Aoki , Tomoki Erata , Kenji Tajima
发明人： Motoaki KAMACHI , Harumi Kamachi , Hirobumi Aoki , Tomoki Erata , Kenji Tajima
IPC分类号： C12P7/62
CPC分类号： C12P13/02 , C12N9/1029 , C12P7/625 , C12P13/001
摘要： The present invention relates to a method for acyltransferase reaction in which an acyl group of acyl coenzyme A is transferred to an acyl group receptor characterized in that the reaction is carried out by production and/or reproduction of acyl coenzyme A from coenzyme A in a reaction system by a chemical thioester exchange reaction with acylthioester. The present invention, wherein expensive acyl CoA is reproduced nonenzymatically in a reaction system, enables to continuously carry out acyltransferase reaction only by putting a small amount of acyl CoA with a donor and a receptor of an acyl group into a system. Accordingly, the method of the present invention can be applied to an industrial production method of various kinds of compounds including useful biological molecules and synthesis of polymers such as polyester.
摘要翻译：本发明涉及一种酰基转移酶反应的方法，其中酰基辅酶A的酰基被转移到酰基基团受体中，其特征在于反应是通过在反应中由辅酶A生产和/或再生酰基辅酶A 系统通过化学硫酯与酰基硫酯交换反应。其中昂贵的酰基CoA在反应体系中非酶原地再生的本发明使得能够仅通过将少量酰基辅酶A与酰基的供体和受体一起投入系统而连续进行酰基转移酶反应。因此，本发明的方法可以应用于包括有用的生物分子和聚合物如聚酯的合成的各种化合物的工业生产方法。

5. 发明授权

US07476521B2 Method for acyltransferase reaction using acyl coenzyme A 有权
标题翻译：使用酰基辅酶A进行酰基转移酶反应的方法
公开(公告)号：US07476521B2
公开(公告)日：2009-01-13
申请号：US10542733
申请日：2004-01-21
申请人： Motoaki Kamachi , Harumi Kamachi , Hirobumi Aoki , Tomoki Erata , Kenji Tajima
发明人： Motoaki Kamachi , Harumi Kamachi , Hirobumi Aoki , Tomoki Erata , Kenji Tajima
IPC分类号： C12P7/62 , C12P7/64
CPC分类号： C12P13/02 , C12N9/1029 , C12P7/625 , C12P13/001
摘要： The present invention relates to a method for acyltransferase reaction in which an acyl group of acyl coenzyme A is transferred to an acyl group receptor characterized in that the reaction is carried out by production and/or reproduction of acyl coenzyme A from coenzyme A in a reaction system by a chemical thioester exchange reaction with acylthioester. The present invention, wherein expensive acyl CoA is reproduced nonenzymatically in a reaction system, enables to continuously carry out acyltransferase reaction only by putting a small amount of acyl CoA with a donor and a receptor of an acyl group into a system. Accordingly, the method of the present invention can be applied to an industrial production method of various kinds of compounds including useful biological molecules and synthesis of polylmers such as polyester.
摘要翻译：本发明涉及一种酰基转移酶反应的方法，其中酰基辅酶A的酰基被转移到酰基基团受体中，其特征在于反应是通过在反应中由辅酶A生产和/或再生酰基辅酶A 系统通过化学硫酯与酰基硫酯交换反应。其中昂贵的酰基CoA在反应体系中非酶原地再生的本发明使得能够仅通过将少量酰基辅酶A与酰基的供体和受体一起投入系统而连续进行酰基转移酶反应。因此，本发明的方法可以应用于包括有用的生物分子和聚合物如聚酯的合成的各种化合物的工业生产方法。

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