专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07101558B2 High yield pertussis vaccine production strain and method for making same 有权
标题翻译：高产率百日咳疫苗生产菌株及其制备方法
公开(公告)号：US07101558B2
公开(公告)日：2006-09-05
申请号：US10312272
申请日：2001-06-26
申请人： Tod J. Merkel , Jerry M. Keith , Xiaoming Yang
发明人： Tod J. Merkel , Jerry M. Keith , Xiaoming Yang
IPC分类号： A61K39/02 , A01N63/00
CPC分类号： C12N1/20 , C07K14/235 , C12N15/74
摘要： The present invention provides a vaccine production strain of Bordetella bronchiseptica that produces a pertussis toxin in high yield. The present invention provides a method for creating a Bordetella bronchiseptica cell line which produces a Bordetella pertussis toxin comprising the steps of introducing a plasmid containing a DNA encoding antibiotic resistance into a Bordetella bronchiseptica strain, selecting for isolates in which the DNA encoding antibiotic resistance is recombinantly incorporated into the chromosome in place of the Bordetella bronchiseptica toxin gene, introducing a plasmid containing DNA encoding subunits of the Bordetella pertussis toxins into the Bordetella bronchiseptica isolates; and, selecting for isolates in which DNA encoding Bordetella pertussis toxin subunit is recombinantly incorporated into the chromosome, the resulting cells producing the Bordetella pertussis toxin. The present invention further provides a method for creating a Bordetella bronchiseptica cell line which produces a Bordetella pertussis toxin and does not express filamentous hemagglutinin.
摘要翻译：本发明提供了以高产率产生百日咳毒素的支气管炎博德特氏菌疫苗生产菌株。本发明提供了一种产生博德特氏菌百日咳杆菌毒素的博德特氏菌细胞系的方法，包括将含有编码抗生素抗性的DNA的质粒导入支气管炎博德特氏菌菌株，选择其中编码抗生素抗性的DNA重组的分离株引入染色体以代替支气管炎博德特氏菌毒素基因，将含有编码百日咳杆菌毒素的亚基的DNA的质粒引入支气管炎博德特菌分离株; 并且选择其中编码百日咳博德特氏菌毒素亚基的DNA重组并入染色体的分离物，所得细胞产生百日咳博德特氏菌毒素。本发明还提供一种产生支气管炎博德特氏菌细胞系的方法，其产生百日咳杆菌毒素并且不表达丝状血凝素。

IPRDB

热门服务

关于我们

友情链接

联系方式