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    • 3. 发明申请
    • Method for molecular delivery into cells using naonotube spearing
    • 使用无穗毛刺将分子递送至细胞的方法
    • US20090311767A1
    • 2009-12-17
    • US11919025
    • 2006-04-21
    • Thomas C. Chiles
    • Thomas C. Chiles
    • C12N13/00
    • C12M35/04
    • The present invention discloses a method of delivering a macromolecule to a cell, comprising engaging the macromolecule to a nanotube wherein the nanotube comprises a magnetic particle, placing the nanotubes into a sample wherein the sample comprises a plurality of cells and applying a force to the sample wherein the force causes the nanotubes to collide with the cells and thereby spear the cells. The method further discloses applying a second force which drives the nanotubes into the cell. Furthermore, the invention discloses a method of transfecting and/or transducing a cell, comprising engaging a macromolecule to a nanotube, placing the nanotubes into communication with the cells and applying a force to the nanotubes which causes the nanotubes to collide with the cells and thereby pierce the cellular membrane. Once inside the cell, the macromolecule may disengage from the nanotube.
    • 本发明公开了一种将大分子递送到细胞的方法,包括使大分子与纳米管接合,其中纳米管包括磁性颗粒,将纳米管放入样品中,其中样品包含多个细胞并向样品施加力 其中所述力使所述纳米管与所述细胞碰撞并由此牵拉所述细胞。 该方法还公开了将驱动纳米管的第二力施加到电池中。 此外,本发明公开了一种转染和/或转导细胞的方法,包括将大分子与纳米管接合,将纳米管与细胞连通并向纳米管施加力,导致纳米管与细胞碰撞,从而 刺穿细胞膜。 一旦在电池内部,大分子可能与纳米管分离。