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1. 发明授权

US10488330B2 Buffer-and-salt-content-independent UV-VIS spectroscopic characterisation of sample DNA and/or RNA content using reference samples having distinct buffer and/or salt content 有权
公开(公告)号：US10488330B2
公开(公告)日：2019-11-26
申请号：US14424570
申请日：2013-08-30
申请人： TRINEAN NV
发明人： Tom Boonefaes
IPC分类号： G01N21/33 , C12Q1/68
CPC分类号： G01N21/33 , C12Q1/68 , G01N2201/12 , Y10T436/143333 , C12Q1/68 , C12Q2527/119 , C12Q2537/165 , C12Q2545/113
摘要： A method is described for characterising a sample comprising at least DNA and/or RNA. The method comprises obtaining a UV-VIS spectroscopic measurement of the sample comprising at least DNA and/or RNA, deriving an absorption parameter based on the UV-VIS spectroscopic measurement, and determining a DNA and/or RNA quantification present in the sample. Determining a DNA and/or RNA quantification thereby comprises taking into account a buffer and/or salt effect on the UV-VIS spectroscopic measurement irrespective of the actual buffer and salt content of the sample, based on said derived absorption parameter and reference spectral data including spectral information for buffer and/or salt effects.

2. 发明申请

US20150226664A1 OPTICAL CHARACTERISATION OF DNA AND/OR RNA 审中-公开
标题翻译： DNA和/或RNA的光学特征
公开(公告)号：US20150226664A1
公开(公告)日：2015-08-13
申请号：US14424570
申请日：2013-08-30
申请人： TRINEAN NV
发明人： Tom Boonefaes
IPC分类号： G01N21/33
CPC分类号： G01N21/33 , C12Q1/68 , G01N2201/12 , Y10T436/143333 , C12Q2527/119 , C12Q2537/165 , C12Q2545/113
摘要： A method is described for characterising a sample comprising at least DNA and/or RNA. The method comprises obtaining a UV-VIS spectroscopic measurement of the sample comprising at least DNA and/or RNA, deriving an absorption parameter based on the UV-VIS spectroscopic measurement, and determining a DNA and/or RNA quantification present in the sample. Determining a DNA and/or RNA quantification thereby comprises taking into account a buffer and/or salt effect on the UV-VIS spectroscopic measurement irrespective of the actual buffer and salt content of the sample, based on said derived absorption parameter and reference spectral data including spectral information for buffer and/or salt effects.
摘要翻译：描述了用于表征包含至少DNA和/或RNA的样品的方法。该方法包括获得包含至少DNA和/或RNA的样品的UV-VIS光谱测量，基于UV-VIS光谱测量导出吸收参数，以及确定样品中存在的DNA和/或RNA定量。基于所述衍生的吸收参数和参考光谱数据，确定DNA和/或RNA定量因此包括考虑对UV-VIS光谱测量的缓冲液和/或盐影响，而不考虑样品的实际缓冲液和盐含量，包括用于缓冲和/或盐效应的光谱信息。

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