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    • 4. 发明申请
    • CELL TREATMENT SOLUTION AND METHOD OF PREPARING STAINED CELL SUSPENSION FOR A MEASUREMENT OF NUCLEAR DNA BY FLOW CYTOMETRY
    • 细胞处理方法和制备用于通过流式细胞术检测核DNA测定的细胞悬浮液的方法
    • US20110151446A1
    • 2011-06-23
    • US12702262
    • 2010-02-08
    • Takahiro SHIOYAMASunao TAKEDAAkane SUZUKINaoki KOBAYASHIYuko NAGAI
    • Takahiro SHIOYAMASunao TAKEDAAkane SUZUKINaoki KOBAYASHIYuko NAGAI
    • C12Q1/68
    • C12Q1/68C12Q2563/107C12Q2565/626
    • A cell treatment solution and a method that is used for preparing a stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry. The cell treatment solution may include a surfactant, RNase, and a fluorescent dye. The surfactant may include, for example, a non-ionic surfactant, a zwitterionic surfactant, an anionic surfactant, and/or a cationic surfactant. In one method of the invention, stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry is prepared. The method may include adding a tissue sample to a cell treatment solution including a surfactant, RNase, and fluorescent dye, disaggregating the tissue sample, and filtering the disaggregated tissue sample. Another method of the invention includes disaggregating a tissue sample, preparing cell suspension by filtering the disaggregated tissue sample, and adding a cell treatment solution including a surfactant, RNase, and fluorescent dye.
    • 一种细胞处理溶液和用于制备通过流式细胞术提供给核DNA测量的染色细胞悬液的方法。 细胞处理溶液可以包括表面活性剂,核糖核酸酶和荧光染料。 表面活性剂可以包括例如非离子表面活性剂,两性离子表面活性剂,阴离子表面活性剂和/或阳离子表面活性剂。 在本发明的一种方法中,制备通过流式细胞术提供给核DNA测量的染色细胞悬浮液。 该方法可以包括将组织样品添加到细胞处理溶液中,所述细胞处理溶液包括表面活性剂,核糖核酸酶和荧光染料,分解组织样品,以及过滤分解的组织样品。 本发明的另一种方法包括分解组织样品,通过过滤分解的组织样品制备细胞悬浮液,并加入包含表面活性剂,核糖核酸酶和荧光染料的细胞处理溶液。