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    • 1. 发明授权
    • Pseudomonas fluorescens lipase
    • 荧光假单胞菌脂肪酶
    • US5534427A
    • 1996-07-09
    • US923120
    • 1992-07-31
    • Robert M. JohnstonSteven L. GoldbergPaul M. Cino
    • Robert M. JohnstonSteven L. GoldbergPaul M. Cino
    • C12N9/20C12N15/55C12N15/70
    • C12N9/20
    • Nucleic acid sequences, particularly DNA sequences, coding for all or part of a lipase of Pseudomonas fluorescens, wherein said lipase has the amino acid sequence encoded by the nucleotide sequence of the BamHI/HindIII insert of the plasmid pRJ-Ltac1 from Escherichia coli BL21/pRJ-Ltac1 deposited with the American Type Culture Collection, expression vectors containing the DNA sequences, host cells containing the expression vectors, and methods utilizing these materials. The invention also concerns polypeptide molecules comprising all or part of a lipase of Pseudomonas fluorescens, wherein said lipase has the amino acid sequence encoded by the nucleotide sequence of the plasmid pRJ-Ltac1 from Escherichia coli BL21/pRJ-Ltac1 BamHI/HindIII insert of deposited with the American Type Culture Collection, and methods for producing these polypeptides.
    • 核酸序列,特别是DNA序列,其编码荧光假单胞菌的全部或部分脂肪酶,其中所述脂肪酶具有由来自大肠杆菌BL21 / P21的质粒pRJ-Ltac1的BamHI / HindIII插入片段的核苷酸序列编码的氨基酸序列, 保藏在美国典型培养物保藏中心的pRJ-Ltac1,含有DNA序列的表达载体,含有表达载体的宿主细胞,以及利用这些材料的方法。 本发明还涉及包含荧光假单胞菌的全部或部分脂肪酶的多肽分子,其中所述脂肪酶具有由大肠杆菌BL21 / pRJ-Ltac1 BamHI / HindIII插入片段的沉积的质粒pRJ-Ltac1的核苷酸序列编码的氨基酸序列 美国典型培养物保藏中心,以及生产这些多肽的方法。