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1. 发明申请

US20110086779A1 ARRAYS OF PROTEIN CAPTURE AGENTS AND METHODS OF USE THEREOF 审中-公开
标题翻译：蛋白质捕获剂的阵列及其使用方法
公开(公告)号：US20110086779A1
公开(公告)日：2011-04-14
申请号：US12950698
申请日：2010-11-19
申请人： Peter Wagner , Steffen Nock , Dana Ault-Riche , Christian Itin
发明人： Peter Wagner , Steffen Nock , Dana Ault-Riche , Christian Itin
IPC分类号： C40B30/08 , C40B30/00
CPC分类号： G01N33/6845 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00702 , B01J2219/00725 , B82Y5/00 , B82Y30/00 , C07K2319/20 , C40B40/10 , G01N33/54393 , G01N33/551
摘要： Arrays of protein-capture agents useful for the simultaneous detection of a plurality of proteins which are the expression products, or fragments thereof, of a cell or population of cells in an organism are provided. A variety of antibody arrays, in particular, are described. Methods of both making and using the arrays of protein-capture agents are also disclosed. The invention arrays are particularly useful for various proteomics applications including assessing patterns of protein expression and modification in cells.
摘要翻译：提供了可用于同时检测生物体中细胞或细胞群体的表达产物或其片段的多种蛋白质的蛋白质捕获剂的阵列。特别描述了各种抗体阵列。还公开了制备和使用蛋白质捕获剂的阵列的方法。本发明阵列对于各种蛋白质组学应用特别有用，包括评估细胞中蛋白质表达和修饰的模式。

2. 发明申请

US20090047695A1 MICRODEVICES FOR HIGH-THROUGHPUT SCREENING OF BIOMOLECULES 审中-公开
标题翻译：用于生物分子高通量筛选的MICRODEVICES
公开(公告)号：US20090047695A1
公开(公告)日：2009-02-19
申请号：US12173515
申请日：2008-07-15
申请人： Peter Wagner , Dana Ault-Riche , Steffen Nock , Christian Itin
发明人： Peter Wagner , Dana Ault-Riche , Steffen Nock , Christian Itin
IPC分类号： C12Q1/48 , G01N33/68 , G01N33/50 , G01N33/543
CPC分类号： B82Y30/00 , Y10S435/805 , Y10S435/81 , Y10T436/143333
摘要： Methods and devices for the parallel, in vitro screening of biomolecular activity using miniaturized microfabricated devices are provided. The biomolecules that can be immobilized on the surface of the devices of the present invention include proteins, polypeptides, nucleic acids, polysaccharides, phospholipids, and related unnatural polymers of biological relevance. These devices are useful in high-throughput drug screening and clinical diagnostics and are preferably used for the parallel screening of families of related proteins.
摘要翻译：提供了使用微型微加工装置并行，体外筛选生物分子活性的方法和装置。可以固定在本发明装置表面上的生物分子包括具有生物相关性的蛋白质，多肽，核酸，多糖，磷脂和相关的非天然聚合物。这些装置在高通量药物筛选和临床诊断中是有用的，并且优选用于相关蛋白家族的并行筛选。

3. 发明申请

US20090028755A1 MICROFLUIDIC DEVICES AND METHODS 失效
标题翻译：微流体装置和方法
公开(公告)号：US20090028755A1
公开(公告)日：2009-01-29
申请号：US12210389
申请日：2008-09-15
申请人： Paul Jedrzejewski , Steffen Nock , Peter Wagner , Pierre Indermuhle , Frank G. Zaugg
发明人： Paul Jedrzejewski , Steffen Nock , Peter Wagner , Pierre Indermuhle , Frank G. Zaugg
IPC分类号： B01J19/00
CPC分类号： B01L3/0244 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00367 , B01J2219/00369 , B01J2219/00382 , B01J2219/00497 , B01J2219/00504 , B01J2219/00509 , B01J2219/00585 , B01J2219/00587 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00664 , B01J2219/00677 , B01J2219/00702 , B01J2219/00725 , B01L3/0241 , B01L3/0248 , B01L3/0262 , B01L3/5085 , B01L3/5088 , B01L2300/0809 , B01L2300/0848 , B01L2400/022 , B01L2400/0688 , B33Y80/00 , B82Y30/00 , C40B40/10 , C40B60/14 , Y10T436/11 , Y10T436/114165 , Y10T436/117497 , Y10T436/118339 , Y10T436/143333 , Y10T436/156666 , Y10T436/25 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： Embodiments of the invention are directed to microfluidic devices. In one embodiment, a microanalysis chip comprises a body having at least one transfer-separation channel with a channel bottom that has a bottom opening. The transfer-separation channel terminates in a discharge aperture.
摘要翻译：本发明的实施例涉及微流体装置。在一个实施例中，微量分析芯片包括具有至少一个具有底部开口的通道底部的转移分离通道的主体。转印分离通道终止于放电孔。

4. 发明授权

US06682942B1 Microdevices for screening biomolecules 失效
标题翻译：用于筛选生物分子的微型装置
公开(公告)号：US06682942B1
公开(公告)日：2004-01-27
申请号：US09570589
申请日：2000-05-12
申请人： Peter Wagner , Dana Ault-Riche , Steffen Nock , Christian Itin
发明人： Peter Wagner , Dana Ault-Riche , Steffen Nock , Christian Itin
IPC分类号： G01N33543
CPC分类号： B82Y30/00
摘要： Methods for the parallel, in vitro screening of biomolecular activity using miniaturized microfabricated devices are provided. The biomolecules immobilized on the surface of the devices employed in the method of the present invention include proteins, polypeptides, polynucleotides, polysaccharides, phospholipids, and related unnatural polymers of biological relevance. These methods are useful in drug development, functional proteomics studies and clinical diagnostics and are preferably used for the parallel screening of families of related proteins.
摘要翻译：提供了使用微型微型加工装置平行体外筛选生物分子活性的方法。固定在本发明方法中使用的装置表面上的生物分子包括具有生物相关性的蛋白质，多肽，多核苷酸，多糖，磷脂和相关的非天然聚合物。这些方法可用于药物开发，功能蛋白质组学研究和临床诊断，并且优选用于相关蛋白家族的平行筛选。

5. 发明授权

US06475808B1 Arrays of proteins and methods of use thereof 失效
标题翻译：蛋白质阵列及其使用方法
公开(公告)号：US06475808B1
公开(公告)日：2002-11-05
申请号：US09353215
申请日：1999-07-14
申请人： Peter Wagner , Dana Ault-Riche , Steffen Nock , Christian Itin
发明人： Peter Wagner , Dana Ault-Riche , Steffen Nock , Christian Itin
IPC分类号： G01N33543
CPC分类号： G01N33/6845 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00702 , B01J2219/00725 , B82Y5/00 , B82Y30/00 , C07K2319/20 , C40B40/10 , G01N33/54393 , G01N33/551
摘要： Protein arrays for the parallel, in vitro screening of biomolecular activity are provided. Methods of using the protein arrays are also disclosed. On the arrays, a plurality of different proteins, such as different members of a single protein family, are immobilized on one or more organic thinfilms on the substrate surface. The protein arrays are particularly useful in drug development, proteomics, and clinical diagnostics.
摘要翻译：提供了用于平行，体外筛选生物分子活性的蛋白质阵列。还公开了使用蛋白质阵列的方法。在阵列上，多个不同的蛋白质，例如单个蛋白质家族的不同成员，被固定在基底表面上的一个或多个有机薄膜上。蛋白质阵列特别适用于药物开发，蛋白质组学和临床诊断。

6. 发明申请

US20050288491A1 Super-humanized antibodies against respiratory syncytial virus 审中-公开
标题翻译：针对呼吸道合胞病毒的超人源化抗体
公开(公告)号：US20050288491A1
公开(公告)日：2005-12-29
申请号：US11061848
申请日：2005-02-17
申请人： David Wilson , Steffen Nock , James Larrick
发明人： David Wilson , Steffen Nock , James Larrick
IPC分类号： A61K39/42 , C07K16/10 , C07K16/44
CPC分类号： C07K16/1027 , C07K2317/24 , C07K2317/56 , C07K2317/565
摘要： Disclosed herein are humanized antibodies that bind to an epitope on the F protein of respiratory syncytial virus. The humanized antibodies were designed by comparing the canonical CDR structure types of the CDRs from a non-human antibody (HNK20) to the canonical CDR structure types found in the human antibody germline sequences as the basis for selecting human variable region frameworks in a method denoted “super-humanization.” Human antibody variable regions having the same or similar canonical CDR structure types as the non-human CDR provided a subset of candidate sequences from which to select the human frameworks. Chimeric variable regions were made comprising the non-human CDRs grafted in corresponding locations into the human frameworks from the candidate human variable regions. Several humanized antibodies that bind the same antigen as HNK20 and that have low immunogenicity were thereby designed, including examples where the framework sequences have less than 65% amino acid identity to the non-human frameworks.
摘要翻译：本文公开了与呼吸道合胞病毒的F蛋白上的表位结合的人源化抗体。通过将来自非人抗体（HNK20）的CDR的规范CDR结构类型与在人抗体种系序列中发现的规范CDR结构类型进行比较来设计人源化抗体，作为在所述方法中选择人可变区框架的基础 “超人性化”。具有与非人CDR相同或相似的典型CDR结构类型的人抗体可变区提供了选择人骨架的候选序列的子集。制备嵌合可变区，其包含从候选人可变区移植到人框架中的相应位置的非人CDR。由此设计了结合与HNK20相同的抗原并且具有低免疫原性的几种人源化抗体，包括其中框架序列与非人框架具有小于65％氨基酸同一性的实例。

7. 发明申请

US20050147962A1 Display of dimeric proteins on phage 审中-公开
标题翻译：在噬菌体上显示二聚体蛋白质
公开(公告)号：US20050147962A1
公开(公告)日：2005-07-07
申请号：US10717735
申请日：2003-11-19
申请人： Christopher Wagstrom , David Wilson , Steffen Nock
发明人： Christopher Wagstrom , David Wilson , Steffen Nock
IPC分类号： C07K16/00 , C12Q1/70 , C07H21/04 , C07K16/18 , C12N1/21 , C12N15/74
CPC分类号： C07K16/005
摘要： Expression vectors for expressing multimeric polypeptides that are anchored on surfaces of genetically replicable packages are disclosed. The expression vectors include a vector segment encoding a polypeptide sequence having three polypeptide segments. One of the segments contains a cleavable peptide sequence cleavable by a proteolytic agent, and another segment has an anchoring peptide sequence for anchoring the multimeric polypeptide to the surface of the genetically replicable package. The cleavable peptide sequence is cleaved by the proteolytic agent and the first segment associates with the third segment to form the multimeric polypeptide. Also disclosed are methods, host cells, and kits employing the expression vectors.
摘要翻译：公开了用于表达锚定在遗传可复制包装表面上的多聚体多肽的表达载体。表达载体包括编码具有三个多肽片段的多肽序列的载体片段。其中一个片段含有可由蛋白水解酶切割的可切割肽序列，另一个片段具有用于将多聚体多肽锚定于基因可复制包装表面的锚定肽序列。可切割的肽序列被蛋白水解酶切割，第一个片段与第三个片段缔合以形成多聚体多肽。还公开了使用表达载体的方法，宿主细胞和试剂盒。

8. 发明授权

US06780582B1 Arrays of protein-capture agents and methods of use thereof 失效
标题翻译：蛋白质捕获剂的阵列及其使用方法
公开(公告)号：US06780582B1
公开(公告)日：2004-08-24
申请号：US09574692
申请日：2000-05-17
申请人： Peter Wagner , Steffen Nock , Dana Ault-Riche , Christian Itin
发明人： Peter Wagner , Steffen Nock , Dana Ault-Riche , Christian Itin
IPC分类号： G01N33543
CPC分类号： B82Y5/00 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00635 , B01J2219/00637 , B01J2219/00644 , B01J2219/00659 , B01J2219/00702 , B01J2219/00725 , B82Y30/00 , C40B40/10 , G01N33/54393 , G01N33/551
摘要： Arrays of protein-capture agents useful for the simultaneous detection of a plurality of proteins which are the expression products, or fragments thereof, of a cell or population of cells in an organism are provided. A variety of antibody arrays, in particular, are described. Methods of both making and using the arrays of protein-capture agents are also disclosed. The invention arrays are particularly useful for various proteomics applications including assessing patterns of protein expression and modification in cells.
摘要翻译：提供了可用于同时检测生物体中细胞或细胞群的表达产物或其片段的多种蛋白质的蛋白质捕获剂的阵列。特别描述了各种抗体阵列。还公开了制备和使用蛋白质捕获剂的阵列的方法。本发明阵列对于各种蛋白质组学应用特别有用，包括评估细胞中蛋白质表达和修饰的模式。

9. 发明授权

US06720157B2 Chips having elevated sample surfaces 失效
公开(公告)号：US06720157B2
公开(公告)日：2004-04-13
申请号：US09792335
申请日：2001-02-23
申请人： Pierre F. Indermuhle , Frank G. Zaugg , Peter Wagner , Steffen Nock
发明人： Pierre F. Indermuhle , Frank G. Zaugg , Peter Wagner , Steffen Nock
IPC分类号： G01N3353
CPC分类号： B01L3/0244 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00367 , B01J2219/00369 , B01J2219/00382 , B01J2219/00497 , B01J2219/00504 , B01J2219/00509 , B01J2219/00585 , B01J2219/00587 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00664 , B01J2219/00677 , B01J2219/00702 , B01J2219/00725 , B01L3/0241 , B01L3/0248 , B01L3/0262 , B01L3/5085 , B01L3/5088 , B01L2300/0809 , B01L2300/0848 , B01L2400/022 , B01L2400/0688 , B33Y80/00 , B82Y30/00 , C40B40/10 , C40B60/14 , Y10T436/11 , Y10T436/114165 , Y10T436/117497 , Y10T436/118339 , Y10T436/143333 , Y10T436/156666 , Y10T436/25 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： A chip is disclosed. The chip includes a base including a non-sample surface and at least one structure includes a pillar. Each structure includes a sample surface that is elevated with respect to the non-sample surface and each structure may be adapted to receive a sample.

10. 发明授权

US06630358B1 Arrays of proteins and methods of use thereof 失效
公开(公告)号：US06630358B1
公开(公告)日：2003-10-07
申请号：US09570363
申请日：2000-05-12
申请人： Peter Wagner , Dana Ault-Riche , Steffen Nock , Christian Itin
发明人： Peter Wagner , Dana Ault-Riche , Steffen Nock , Christian Itin
IPC分类号： G01N33543
CPC分类号： G01N33/6845 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00702 , B01J2219/00725 , B82Y5/00 , B82Y30/00 , C07K2319/20 , C40B40/10 , G01N33/54393 , G01N33/551
摘要： Protein arrays for the parallel, in vitro screening of biomolecular activity are provided. Methods of using the protein arrays are also disclosed. On the arrays, a plurality of different proteins, such as different members of a single protein family, are immobilized on one or more organic thinfilms on the substrate surface. The protein arrays are particularly useful in drug development, proteomics, and clinical diagnostics.

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