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1. 发明申请

US20140073009A1 Methods for Producing Secreted Polypeptides 有权
标题翻译：生产分泌多肽的方法
公开(公告)号：US20140073009A1
公开(公告)日：2014-03-13
申请号：US14005733
申请日：2012-03-22
申请人： Steen Troels Joergensen , Anne Breüner
发明人： Steen Troels Joergensen , Anne Breüner
IPC分类号： C07K14/32 , C12N9/28
CPC分类号： C07K14/32 , C07K2319/02 , C12N9/2417 , C12N15/625 , C12N15/75 , C12P21/02 , Y02P20/52
摘要： The present invention relates to methods for producing a polypeptide comprising using a signal peptide foreign to the polypeptide, nucleic acid constructs comprising a first and a second nucleotide sequence encoding the signal peptide and the polypeptide and expression vectors and host cells comprising said nucleic acid construct. The signal peptide is the LQ2 peptide which is a hybrid of sequence from the signal peptide of alpha amylase from Bacillus licheniformis and of alpha amylase from Bacillus amyloliquefaciens.
摘要翻译：本发明涉及生产多肽的方法，其包括使用多肽外源的信号肽，包含编码信号肽的第一和第二核苷酸序列和表达载体的核酸构建体和包含所述核酸构建体的宿主细胞。信号肽是来自地衣芽孢杆菌的α淀粉酶的信号肽和来自解淀粉芽孢杆菌的α淀粉酶的序列的杂交的LQ2肽。

2. 发明申请

US20130295644A1 Polypeptides Having Succinyl-CoA: Acetoacetate Transferase Activity and Polynucleotides Encoding Same 有权
标题翻译：具有琥珀酰辅酶A的多肽：乙酰乙酸转移酶活性和编码相同的多核苷酸
公开(公告)号：US20130295644A1
公开(公告)日：2013-11-07
申请号：US13882343
申请日：2011-10-28
申请人： Bjarke Christensen , Peter Bjarke Olsen , Torsten Bak Regueira , Steen Troels Joergensen , Alan Berry
发明人： Bjarke Christensen , Peter Bjarke Olsen , Torsten Bak Regueira , Steen Troels Joergensen , Alan Berry
IPC分类号： C12N9/10
CPC分类号： C12N9/13 , C12Y208/03005
摘要： The present invention relates to isolated protein complexes having succinyl-CoA:acetoacetate transferase activity, isolated polypeptide subunits thereof, and isolated polynucleotides encoding the subunits. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and protein complexes.
摘要翻译：本发明涉及具有琥珀酰-CoA：乙酰乙酸转移酶活性的分离的蛋白复合物，其分离的多肽亚基，以及编码亚基的分离的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽和蛋白质复合物的方法。

3. 发明申请

US20130280775A1 Recombinant N-propanol and Isopropanol Production 审中-公开
标题翻译：重组N-丙醇和异丙醇生产
公开(公告)号：US20130280775A1
公开(公告)日：2013-10-24
申请号：US13882336
申请日：2011-10-28
申请人： Thomas Grotkjaer , Bjarke Christensen , Torsten Bak Regueira , Steen Troels Joergensen , Alan Berry
发明人： Thomas Grotkjaer , Bjarke Christensen , Torsten Bak Regueira , Steen Troels Joergensen , Alan Berry
IPC分类号： C12P7/04
CPC分类号： C12P7/04 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/13 , C12N9/16 , C12N9/88 , C12N9/90 , C12N15/746 , C12P7/02 , C12Y101/01001 , C12Y101/0108 , C12Y102/01003 , C12Y203/01009 , C12Y208/03005 , C12Y208/03009 , C12Y301/02011 , C12Y401/01004 , C12Y401/01041 , C12Y501/99001 , C12Y504/99002
摘要： The present invention relates to methods of producing n-propanol, isopropanol, and coproducing n-propanol with isopropanol. The present invention also relates to methods for producing propylene, as well as host cells capable of n-propanol and isopropanol production.
摘要翻译：本发明涉及用异丙醇生产正丙醇，异丙醇和共同生产正丙醇的方法。本发明还涉及生产丙烯的方法，以及能够进行正丙醇和异丙醇生产的宿主细胞。

4. 发明授权

US08728804B2 Polypeptides having succinyl-CoA: acetoacetate transferase activity and polynucleotides encoding same 有权
标题翻译：具有琥珀酰-CoA：乙酰乙酸转移酶活性的多肽和编码该多肽的多核苷酸
公开(公告)号：US08728804B2
公开(公告)日：2014-05-20
申请号：US13882343
申请日：2011-10-28
申请人： Bjarke Christensen , Peter Bjarke Olsen , Torsten Bak Regueira , Steen Troels Joergensen , Alan Berry
发明人： Bjarke Christensen , Peter Bjarke Olsen , Torsten Bak Regueira , Steen Troels Joergensen , Alan Berry
IPC分类号： C12N15/00 , C07H21/04
CPC分类号： C12N9/13 , C12Y208/03005
摘要： The present invention relates to isolated protein complexes having succinyl-CoA:acetoacetate transferase activity, isolated polypeptide subunits thereof, and isolated polynucleotides encoding the subunits. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and protein complexes.
摘要翻译：本发明涉及具有琥珀酰-CoA：乙酰乙酸转移酶活性的分离的蛋白复合物，其分离的多肽亚基，以及编码亚基的分离的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽和蛋白质复合物的方法。

5. 发明授权

US09428558B2 Methods for producing secreted polypeptides 有权
标题翻译：产生分泌多肽的方法
公开(公告)号：US09428558B2
公开(公告)日：2016-08-30
申请号：US14005733
申请日：2012-03-22
申请人： Steen Troels Joergensen , Anne Breüner
发明人： Steen Troels Joergensen , Anne Breüner
IPC分类号： C12N15/09 , C12N15/10 , C07K14/32 , C12N15/62 , C12N15/75 , C12P21/02 , C12N9/28
CPC分类号： C07K14/32 , C07K2319/02 , C12N9/2417 , C12N15/625 , C12N15/75 , C12P21/02 , Y02P20/52
摘要： The present invention relates to methods for producing a polypeptide comprising using a signal peptide foreign to the polypeptide, nucleic acid constructs comprising a first and a second nucleotide sequence encoding the signal peptide and the polypeptide and expression vectors and host cells comprising said nucleic acid construct. The signal peptide is the LQ2 peptide which is a hybrid of sequence from the signal peptide of alpha amylase from Bacillus licheniformis and of alpha amylase from Bacillus amyloliquefaciens.
摘要翻译：本发明涉及生产多肽的方法，其包括使用多肽外源的信号肽，包含编码信号肽的第一和第二核苷酸序列和表达载体的核酸构建体和包含所述核酸构建体的宿主细胞。信号肽是来自地衣芽孢杆菌的α淀粉酶的信号肽和来自解淀粉芽孢杆菌的α淀粉酶的序列的杂交的LQ2肽。

6. 发明授权

US09334493B2 Selection of well-expressed synthetic genes 有权
标题翻译：选择良好表达的合成基因
公开(公告)号：US09334493B2
公开(公告)日：2016-05-10
申请号：US12516721
申请日：2007-12-20
申请人： Steen Troels Joergensen
发明人： Steen Troels Joergensen
IPC分类号： C12N15/67 , C12N15/10
CPC分类号： C12N15/1086 , C12N15/67
摘要： The invention relates to a method of indirectly identifying a polynucleotide comprising an open reading frame having an improved expression level from a library of polynucleotides encoding the same polypeptide of interest, or variants or homologues thereof, in a host cell expression library, the method comprising the steps of: a) providing an expression library in a host cell, said library comprising at least two different open reading frames encoding the same polypeptide of interest, or variants or homologues thereof, each open reading frame being translationally coupled to a downstream gene encoding a screenable or selectable reporter; b) culturing the host cell under conditions conducive to the expression of the polypeptide of interest, or variant or homologue thereof; and c) screening and/or selecting for a host cell having an improved expression level of the translationally coupled reporter when compared to one or more other host cells of the library, thus indirectly identifying a polynucleotide comprising an open reading frame having an improved expression level in the host cell; as well as the expression libraries and host cells comprising said libraries.
摘要翻译：本发明涉及一种间接鉴定多核苷酸的方法，所述多核苷酸包含在宿主细胞表达文库中从编码相同的目的多肽的多核苷酸文库或其变体或同源物的文库中具有改进的表达水平的开放阅读框，所述方法包括步骤：a）在宿主细胞中提供表达文库，所述文库包含编码相同感兴趣多肽的至少两个不同的开放阅读框，或其变体或同源物，每个开放阅读框与下游基因编码一个可筛选或选择的记者; b）在有利于目的多肽表达的条件下或其变体或同源物条件下培养宿主细胞; 和c）当与文库的一个或多个其他宿主细胞相比时，筛选和/或选择具有改良的翻译结合的报道分子的表达水平的宿主细胞，从而间接鉴定包含具有改善的表达水平的开放阅读框的多核苷酸在宿主细胞中; 以及包含所述文库的表达文库和宿主细胞。

7. 发明授权

US06428981B1 Bacillus protein production cell 有权
标题翻译：芽孢杆菌蛋白生产细胞
公开(公告)号：US06428981B1
公开(公告)日：2002-08-06
申请号：US09542749
申请日：2000-04-04
申请人： Steen Troels Joergensen , Christina Lund Christensen , Tina Kristensen
发明人： Steen Troels Joergensen , Christina Lund Christensen , Tina Kristensen
IPC分类号： C12P2106
CPC分类号： C07K14/32
摘要： A Bacillus cell for improved production of a polypeptide of interest and a process for producing a polypeptide of interest are disclosed.
摘要翻译：公开了用于改善目标多肽的产生的芽孢杆菌细胞和用于产生感兴趣的多肽的方法。

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