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1. 发明授权

US5925544A Method of homologous recombination followed by in vivo selection of DNA amplification 失效
标题翻译：同源重组方法，然后进行DNA扩增的体内选择
公开(公告)号：US5925544A
公开(公告)日：1999-07-20
申请号：US954391
申请日：1997-10-20
申请人： Steen Troels J.o slashed.rgensen
发明人： Steen Troels J.o slashed.rgensen
IPC分类号： C12N15/64 , C12N15/90 , C12P21/00 , C07H21/04 , C12P19/34 , C12Q1/68
CPC分类号： C12N15/64 , C12N15/902
摘要： A method of amplifying in vivo a DNA sequence B present in a genome of a parent cell, comprising (a) integrating in a genome of said cell a DNA construct comprising the structure C-M-A-D, in which both A and C denote a DNA sequence which is homologous with a genomic DNA fragment either flanking or overlapping the DNA sequence B, the sequence C being located in the opposite end the sequence B as compared to A, D denotes a DNA sequence which is homologous with a genomic DNA fragment located distal for C as compared to B, and M denotes a DNA sequence encoding a selection marker, (b) selecting for cells in which the DNA sequence M has been integrated in the genome, which cells comprise, in any orientation, the structure A-B-C-M-A-D and (c) propagating the cells selected in step (b) under increasing selection pressure to obtain a cell which has obtained an increased number of genomically integrated copies of the DNA sequences B and M.
摘要翻译：在体内扩增存在于亲本细胞基因组中的DNA序列B的方法，包括（a）在所述细胞的基因组中整合含有结构CMAD的DNA构建体，其中A和C都表示DNA序列，与DNA序列B侧翼或重叠的基因组DNA片段同源，与A相比，序列C位于序列B的相对端，D表示与位于C末端的基因组DNA片段同源的DNA序列与B相比，M表示编码选择标记的DNA序列，（b）选择其中DNA序列M已经整合到基因组中的细胞，该细胞以任何方向包含结构ABCMAD和（c）传播的结构在增加的选择压力下在步骤（b）中选择的细胞，以获得获得DNA序列B和M的增加的基因整合拷贝数的细胞。

2. 发明授权

US5882888A DNA integration by transposition 失效
标题翻译：通过转座进行DNA整合
公开(公告)号：US5882888A
公开(公告)日：1999-03-16
申请号：US875154
申请日：1997-07-17
申请人： Steen Troels J.o slashed.rgensen
发明人： Steen Troels J.o slashed.rgensen
IPC分类号： C12N15/09 , C12N1/21 , C12N15/75 , C12N15/90 , C12P21/02 , C12R1/07 , C12R1/225 , C12P21/00 , C07H21/04 , C12N15/70
CPC分类号： C12N15/75 , C12N15/90
摘要： Multicopy strains of gram-positive bacteria carrying multiple copies of a DNA sequence of interest may be constructed by use of a method involving introduction of a DNA construct comprising the DNA sequence of interest into the genome of the recipient cell by transposition and subsequent deletion of a marker gene used for selection of the cells having received the DNA construct by a resolution system. The multicopy strains are preferably free from a gene encoding an undesirable marker such as an antibiotic resistance marker.
摘要翻译： PCT No.PCT / DK96 / 00038 Sec。 371日期1997年7月17日 102（e）日期1997年7月17日PCT 1996年1月23日PCT PCT。公开号WO96 / 23073 日期1996年8月1日携带多个目的DNA序列拷贝的革兰氏阳性细菌的多拷贝菌株可以通过使用包括通过转座将包含感兴趣的DNA序列的DNA构建体引入受体细胞的基因组的方法来构建以及随后删除用于通过分辨系统选择接受DNA构建体的细胞的标记基因。多拷贝菌株优选不含编码不良标志物如抗生素抗性标记物的基因。

3. 发明授权

US5955367A Production of bacillus thuringiensis integrants 失效
标题翻译：苏云金芽孢杆菌整合素的生产
公开(公告)号：US5955367A
公开(公告)日：1999-09-21
申请号：US60288
申请日：1998-04-14
申请人： Lee Fremont Adams , Michael David Thomas , Alan P. Sloma , William R. Widner , Steen Troels J.o slashed.rgensen , Per Lin.ang. J.o slashed.rgensen , B.o slashed.rge Krag Diderichsen
发明人： Lee Fremont Adams , Michael David Thomas , Alan P. Sloma , William R. Widner , Steen Troels J.o slashed.rgensen , Per Lin.ang. J.o slashed.rgensen , B.o slashed.rge Krag Diderichsen
IPC分类号： A01N63/00 , C12N1/21 , C12N15/32 , C12N15/74 , C12N15/90
CPC分类号： A01N63/00
摘要： The invention relates to a method for producing an integrant(s) of Bacillus thuringiensis. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.
摘要翻译：本发明涉及一种用于生产苏云金芽孢杆菌的整合体的方法。本发明还涉及这样的整合剂，包含这种整合剂的组合物以及使用这些组合物来控制害虫的方法。

4. 发明授权

US5733753A Amplification of genomic DNA by site specific integration of a selectable marker construct 失效
标题翻译：通过选择性标记构建体的位点特异性整合扩增基因组DNA
公开(公告)号：US5733753A
公开(公告)日：1998-03-31
申请号：US751282
申请日：1996-11-18
申请人： Steen Troels J.o slashed.rgensen
发明人： Steen Troels J.o slashed.rgensen
IPC分类号： C12N15/64 , C12N15/67 , C12N15/90 , C12P19/34 , C07H21/02 , C12Q1/68
CPC分类号： C12N15/67 , C12N15/64 , C12N15/902
摘要： A method of amplifying in vivo a DNA sequence B present in a genome of a parent cell, comprising (a) integrating in a genome of said cell a DNA construct comprising the structure C-M-A-D, in which both A and C denote a DNA sequence which is homologous with a genomic DNA fragment either flanking or overlapping the DNA sequence B, the sequence C being located in the opposite end the sequence B as compared to A, D denotes a DNA sequence which is homologous with a genomic DNA fragment located distal for C as compared to B, and M denotes a DNA sequence encoding a selection marker, (b) selecting for cells in which the DNA sequence M has been integrated in the genome, which cells comprise, in any orientation, the structure A-B-C-M-A-D and (c) propagating the cells selected in step (b) under increasing selection pressure to obtain a cell which has obtained an increased number of genomically integrated copies of the DNA sequences B and M.
摘要翻译：在体内扩增存在于亲本细胞基因组中的DNA序列B的方法，包括（a）在所述细胞的基因组中整合含有结构CMAD的DNA构建体，其中A和C都表示DNA序列，与DNA序列B侧翼或重叠的基因组DNA片段同源，与A相比，序列C位于序列B的相对端，D表示与位于C末端的基因组DNA片段同源的DNA序列与B相比，M表示编码选择标记的DNA序列，（b）选择其中DNA序列M已经整合到基因组中的细胞，该细胞以任何方向包含结构ABCMAD和（c）传播的结构在增加的选择压力下在步骤（b）中选择的细胞，以获得获得DNA序列B和M的增加的基因整合拷贝数的细胞。

5. 发明授权

US5681715A Process for preparing lipases 失效
标题翻译：脂肪酶的制备方法
公开(公告)号：US5681715A
公开(公告)日：1997-10-28
申请号：US400422
申请日：1995-03-03
申请人： Steen Troels J.o slashed.rgensen , Boerge Krag Diderichsen , Catherine M. Buckley , Audrey Hobson , David J. McConnell
发明人： Steen Troels J.o slashed.rgensen , Boerge Krag Diderichsen , Catherine M. Buckley , Audrey Hobson , David J. McConnell
IPC分类号： C12N15/09 , C07K14/21 , C12N20060101 , C12N1/21 , C12N9/20 , C12N15/00 , C12N15/55 , C12P19/38 , C12R1/07 , C12R1/19 , C12R1/38 , C12N15/52 , C12N15/31
CPC分类号： C07K14/21 , C12N9/20 , C07K2319/00 , C07K2319/35 , C07K2319/91
摘要： A process for producing an active lipase enzyme in vitro, comprising mixing an inactive or partly active lipase enzyme with a chaperone molecule and subjecting the mixture to denaturation followed by renaturation to produce the active lipase enzyme.
摘要翻译：在体外制备活性脂肪酶的方法，包括将无活性或部分活性脂肪酶与分子伴侣分子混合并使混合物变性，然后复性以产生活性脂肪酶。

6. 发明授权

US6100063A Procaryotic cell comprising at least two copies of a gene 有权
标题翻译：包含至少两个拷贝的基因的原核细胞
公开(公告)号：US6100063A
公开(公告)日：2000-08-08
申请号：US248643
申请日：1999-02-11
申请人： Steen Troels J.o slashed.rgensen
发明人： Steen Troels J.o slashed.rgensen
IPC分类号： C12N15/74 , C12N15/75 , C12P21/02
CPC分类号： C12N15/75 , C12N15/74
摘要： A prokaryotic cell expressing a gene of interest and comprising at least two copies of said gene on the chromosome.
摘要翻译：表达感兴趣的基因并且在染色体上包含所述基因的至少两个拷贝的原核细胞。

7. 发明授权

US5698415A Bacillus promoter derived from a variant of a bacillus licheniformis X-amylase promoter 失效
标题翻译：衍生自地衣芽孢杆菌X-淀粉酶启动子变异体的芽孢杆菌启动子
公开(公告)号：US5698415A
公开(公告)日：1997-12-16
申请号：US240748
申请日：1994-05-10
申请人： Steen Troels J.o slashed.rgensen , B.o slashed.rge Krag Diderichsen
发明人： Steen Troels J.o slashed.rgensen , B.o slashed.rge Krag Diderichsen
IPC分类号： C11D1/14 , C11D3/386 , C12N9/10 , C12N9/28 , C12N9/56 , C12N15/00 , C12N15/09 , C12N15/75 , C12P21/00 , C12R1/10 , C12R1/19 , C12P21/06 , C12N1/21 , C12N15/11
CPC分类号： C12N9/2417 , C12N15/75 , C12N9/1074 , C07K2319/00 , C07K2319/02
摘要： A Bacillus promoter included in DNA sequence (SEQ ID#1), wherein each of N.sup.1 -N.sup.9 is A, T, C or G with the exception that N.sup.2 -N.sup.9 do not together form the sequence ATGTTTCA or GTGTTTCA, or a functional homologue of said sequence.
摘要翻译： PCT No.PCT / DK92 / 00338 Sec。 371日期1994年5月10日 102（e）日期1994年5月10日PCT提交1992年11月13日PCT公布。公开号WO93 / 10249 日期：1993年5月27日包含在DNA序列（SEQ ID＃1）中的芽孢杆菌启动子，其中N1-N9各自为A，T，C或G，不同之处在于N2-N9不一起形成ATGTTTCA或GTGTTTCA序列，或所述序列的功能同源物。

8. 发明授权

US5695976A Stable integration of DNA in bacterial genomes 失效
标题翻译： DNA在细菌基因组中的稳定整合
公开(公告)号：US5695976A
公开(公告)日：1997-12-09
申请号：US441714
申请日：1995-05-15
申请人： Steen Troels J.o slashed.rgensen , Per Lin.ang. J.o slashed.rgensen , B.o slashed.rge Krag Diderichsen
发明人： Steen Troels J.o slashed.rgensen , Per Lin.ang. J.o slashed.rgensen , B.o slashed.rge Krag Diderichsen
IPC分类号： C12N15/75 , C12N15/64 , C12N15/65 , C12P21/02
CPC分类号： C12N15/75
摘要： A bacterial cell which in its genome carries an integrated non-replicative DNA construct comprising (1) a DNA sequence of interest, (2) a DNA sequence which is homologous with a region of the genome of the cell, and (3) an origin of replication, the DNA construct lacking a functional gene coding for a factor required to initiate replication from the origin of replication.
摘要翻译：在其基因组中携带整合的非复制DNA构建体的细菌细胞，其包含（1）感兴趣的DNA序列，（2）与细胞基因组区域同源的DNA序列，和（3）起源的复制，缺乏编码启动从复制起点复制所需的因子的功能基因的DNA构建体。

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