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1. 发明申请

US20050095669A1 Eubacterial rna-polymerase mutants with altered product production 审中-公开
标题翻译：具有改变产品生产的真菌rna聚合酶突变体
公开(公告)号：US20050095669A1
公开(公告)日：2005-05-05
申请号：US10498302
申请日：2002-12-20
申请人： Steen Joergensen , Jens Toenne , Niels Banke , Preben Nielsen
发明人： Steen Joergensen , Jens Toenne , Niels Banke , Preben Nielsen
IPC分类号： C12N15/01 , C12N1/20 , C12N1/21 , C12N9/10 , C12N9/12 , C12P21/02 , C12N15/74
CPC分类号： C12N9/1247 , C12N9/2408 , C12N9/2417 , C12N9/54
摘要： The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of the positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymererase beta-sub-unit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.
摘要翻译：本发明涉及分离的突变体真细胞，其包含至少一个导致由rpoB基因编码的RNA聚合酶的β-亚基中的至少一个氨基酸取代的突变体，提供改变产生感兴趣的产物当所述感兴趣的产物的生产与在相同条件下生长的等基因野生型菌株中相同产物的产生进行比较时，其中至少一个氨基酸的取代发生在任何位置469,478,482,485 ，或SEQ ID NO：2的487，或在任何细菌RNA-多聚赖氨酸酶β-亚单位家族成员中的等同位置。本发明的另一方面涉及用于产生至少一种在突变体杆菌中感兴趣的产物的方法和本发明的突变型杆菌的用途，用于生产至少一种目标产物。

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