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    • 3. 发明申请
    • Arabinogalactan Protein Compositions and Methods for Fostering Somatic Embryogenic Competence
    • 阿拉伯半乳聚糖蛋白质组合物和促进体细胞胚胎发生能力的方法
    • US20080124800A1
    • 2008-05-29
    • US10594418
    • 2005-03-31
    • Simon PoonRobyn Louise HeathAdrienne E. Clarke
    • Simon PoonRobyn Louise HeathAdrienne E. Clarke
    • C12N5/02C07K14/415C12N5/04
    • C07K14/415A01H4/001A01H4/005A01H4/008
    • Methods for fostering somatic embryogenic competence of a plant cell or tissue by contacting the plant cell or tissue with an arabinogalactan protein (AGP) composition effective for fostering somatic embryogenic competence in plant species and varieties that are recalcitrant to somatic embryogenesis and/or regeneration are provided. AGP compositions useful for fostering somatic embryogenic competence are provided, including total AGP and an AGP RP-HPLC hydrophobic fraction, both from embryogenic callus, from plant varieties including cotton lines Coker 315, Siokra 1-4, and Sicala 40, including at concentrations ranging from about 0.0008 mg/L to about 100 mg/L. Methods for regenerating plants, producing transformed plants, and impeding somatic embryogenic competence are also provided. Plant culture media and methods for making useful AGP compositions and media are also provided. Amino acid and nucleotide sequences for peptides and proteins eluting in hydrophobic AGP fractions are also provided.
    • 提供了通过使植物细胞或组织与有效促进体内胚胎发生能力的阿拉伯半乳聚糖蛋白质(AGP)组合来培养植物细胞或组织的体细胞胚发生能力的方法,所述组合物能够在体细胞胚发生和/或再生中顽抗的植物物种和品种中产生体细胞胚发生能力 。 提供了可用于促进体细胞胚发生能力的AGP组合物,包括来自胚性愈伤组织的总AGP和AGP RP-HPLC疏水部分,来自包括棉花品系Coker 315,Siokra 1-4和Sicala 40的植物品种的AGP组合,包括浓度范围 约0.0008mg / L至约100mg / L。 还提供了再生植物,产生转化植物以及阻碍体细胞胚发生能力的方法。 还提供植物培养基和制备有用的AGP组合物和培养基的方法。 还提供了用疏水AGP级分洗脱的肽和蛋白质的氨基酸和核苷酸序列。