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1. 发明申请

US20120065439A1 SYSTEM FOR PHOTOBIOSYNTHETIC PRODUCTION, SEPARATION AND SATURATION OF CARBONACEOUS CHEMICALS AND FUELS 审中-公开
标题翻译：碳氢化合物和燃料的光合作用生产，分离和饱和系统
公开(公告)号：US20120065439A1
公开(公告)日：2012-03-15
申请号：US13203717
申请日：2010-03-01
申请人： Christian Siemer , Simon Eric Aspland , Annastasiah Mudiwa Mhaka , Philip Goelet
发明人： Christian Siemer , Simon Eric Aspland , Annastasiah Mudiwa Mhaka , Philip Goelet
IPC分类号： C07C11/10 , C12M1/42 , C07C9/18 , C12P5/02
CPC分类号： C12P5/007 , C12M21/02 , C12M23/04 , C12M23/06 , C12M29/22 , C12M43/06
摘要： The present invention provides new energy solutions that are sustainable both environmentally and economically. The invention relates to photo-biocatalytic (PBC) methods and systems designed to produce and isolate carbonaceous chemicals using carbon dioxide, sunlight, and genetically engineered photosynthetic microorganisms. The PBC system comprises of procedural, mechanical and biological components designed for the production of carbonaceous chemicals. In an exemplary embodiment, the system includes a photo-biochemical reactor designed to maintain the genetically modified photosynthetic microorganisms in the optimal condition to capture carbon dioxide and convert it into metabolic intermediates using energy from sunlight, convert the metabolic intermediates into isoprene using recombinant enzymes, allow for the release of isoprene from cells, capture, separate and concentrate isoprene, and ultimately collect the isoprene at levels and in a form that would serve as a viable alternative to petroleum-dependent energy.
摘要翻译：本发明提供了在环境和经济上可持续的新能源解决方案。本发明涉及光生物催化（PBC）方法和系统，其设计用于使用二氧化碳，阳光和基因工程的光合微生物来生产和分离碳质化学品。 PBC系统包括用于生产含碳化学物质的程序，机械和生物部件。在一个示例性实施方案中，该系统包括光生化反应器，其设计用于将经过遗传修饰的光合微生物保持在最佳条件下以捕获二氧化碳并使用来自阳光的能量将其转化为代谢中间体，使用重组酶将代谢中间体转化为异戊二烯，允许从细胞释放异戊二烯，捕获，分离和浓缩异戊二烯，并最终收集异戊二烯的水平和形式，作为石油依赖能量的可行替代品。

2. 发明申请

US20090305402A1 Methods and compositions for using zinc finger endonucleases to enhance homologous recombination 审中-公开
标题翻译：使用锌指核酸内切酶增强同源重组的方法和组合物
公开(公告)号：US20090305402A1
公开(公告)日：2009-12-10
申请号：US12456111
申请日：2009-06-11
申请人： Monika Liljedahl , Simon Eric Aspland , David J. Segal
发明人： Monika Liljedahl , Simon Eric Aspland , David J. Segal
IPC分类号： C12N5/10 , C12N5/04 , C12N5/06 , C12N5/08
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2217/075 , C07K2319/00 , C12N9/22 , C12N15/8213 , C12N15/907
摘要： Described herein are methods of generating a genetically modified cell by providing a zinc finger endonuclease (ZFE) that includes an endonuclease domain that cuts DNA, and a zinc finger domain that includes a plurality of zinc fingers that bind to a specific nucleotide sequence within the endogenous chromosomal target DNA in the primary cell. Further, the methods can include contacting the endogenous chromosomal target DNA sequence with the zinc finger endonuclease in the primary cell such that the zinc finger endonuclease cuts both strands of a nucleotide sequence within the endogenous chromosomal target DNA sequence in the primary cell, thereby enhancing the frequency of homologous recombination in the endogenous chromosomal target DNA sequence.
摘要翻译：本文描述的是通过提供包含切割DNA的内切核酸酶结构域的锌指内切核酸酶（ZFE）和锌指结构域来产生遗传修饰的细胞的方法，锌指结构域包括结合内源性特异性核苷酸序列的多个锌指原代细胞染色体靶DNA。此外，所述方法可以包括使内源染色体靶DNA序列与原代细胞中的锌指内切核酸酶接触，使得锌指内切核酸酶切割原代细胞内的内源性染色体靶DNA序列内的核苷酸序列的两条链，从而增强在内源染色体靶DNA序列中同源重组的频率。

3. 发明申请

US20080209587A1 Methods and compositions for using zinc finger endonucleases to enhance homologous recombination 审中-公开
标题翻译：使用锌指核酸内切酶增强同源重组的方法和组合物
公开(公告)号：US20080209587A1
公开(公告)日：2008-08-28
申请号：US11975017
申请日：2007-10-17
申请人： Monika Liljedahl , Simon Eric Aspland , David J. Segal
发明人： Monika Liljedahl , Simon Eric Aspland , David J. Segal
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2217/075 , C07K2319/00 , C12N9/22 , C12N15/8213 , C12N15/907
摘要： Embodiments relate to methods of generating a genetically modified cell. The methods can include providing a primary cell containing an endogenous chromosomal target DNA sequence in which it is desired to have homologous recombination occur. The methods also can include providing a zinc finger endonuclease (ZFE) that includes an endonuclease domain that cuts DNA, and a zinc finger domain that includes a plurality of zinc fingers that bind to a specific nucleotide sequence within the endogenous chromosomal target DNA in the primary cell. Further, the methods can include contacting the endogenous chromosomal target DNA sequence with the zinc finger endonuclease in the primary cell such that the zinc finger endonuclease cuts both strands of a nucleotide sequence within the endogenous chromosomal target DNA sequence in the primary cell, thereby enhancing the frequency of homologous recombination in the endogenous chromosomal target DNA sequence. The methods also include providing a nucleic acid comprising a sequence homologous to at least a portion of said endogenous chromosomal target DNA such that homologous recombination occurs between the endogenous chromosomal target DNA sequence and the nucleic acid.
摘要翻译：实施方案涉及产生遗传修饰细胞的方法。所述方法可以包括提供含有其中期望具有同源重组的内源性染色体靶DNA序列的原代细胞。所述方法还可以包括提供包括切割DNA的核酸内切酶结构域的锌指内切核酸酶（ZFE），以及锌指结构域，其包含多个锌指，所述锌指结合到主要内源性染色体靶DNA中的特定核苷酸序列细胞。此外，所述方法可以包括使内源染色体靶DNA序列与原代细胞中的锌指内切核酸酶接触，使得锌指内切核酸酶切割原代细胞内的内源性染色体靶DNA序列内的核苷酸序列的两条链，从而增强在内源染色体靶DNA序列中同源重组的频率。所述方法还包括提供包含与所述内源染色体靶DNA的至少一部分同源的序列的核酸，使得在内源性染色体靶DNA序列与核酸之间发生同源重组。

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