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1. 发明授权

US09448241B2 Mass spectrometry method for a polypeptide including a cysteine residue 有权
标题翻译：包含半胱氨酸残基的多肽的质谱法
公开(公告)号：US09448241B2
公开(公告)日：2016-09-20
申请号：US13576124
申请日：2011-03-28
申请人： Jumpei Naito , Shuya Satoh , Manabu Komatsu , Hiroyuki Hashimoto
发明人： Jumpei Naito , Shuya Satoh , Manabu Komatsu , Hiroyuki Hashimoto
IPC分类号： G01N33/68 , H01J49/00
CPC分类号： G01N33/6851 , H01J49/00 , Y10T436/182
摘要： The problem to be solved by the present invention is to provide a method for treating a polypeptide including a cysteine residue for enhancing the sensitivity of detection thereof in mass spectrometry using charged particles. A mass spectrometry method for a polypeptide including a cysteine residue is provided which includes treating the polypeptide including a cysteine residue with a cyanation agent, treating the cyanated polypeptide with a base, and then desorption ionizing the base-treated polypeptides using charged particles for mass spectrometry.
摘要翻译：本发明要解决的问题是提供一种用于在使用带电粒子的质谱法中治疗包含半胱氨酸残基的多肽以提高其检测灵敏度的方法。提供了包括半胱氨酸残基的多肽的质谱法，其包括用氰化剂处理包含半胱氨酸残基的多肽，用碱处理氰化多肽，然后使用带电粒子对基质处理的多肽进行解吸，用于质谱法。

2. 发明授权

US08415615B2 Information acquisition method 失效
标题翻译：信息采集方法
公开(公告)号：US08415615B2
公开(公告)日：2013-04-09
申请号：US13351675
申请日：2012-01-17
申请人： Shuya Satoh , Hiroyuki Hashimoto
发明人： Shuya Satoh , Hiroyuki Hashimoto
IPC分类号： H01J49/26
CPC分类号： H01J49/142 , Y10T436/24 , Y10T436/25 , Y10T436/25875
摘要： Provided is a method that achieves both of soft ionization and high ionization efficiency of a substance to be analyzed at each measurement site without impairing a two-dimensional distribution state of the substance to be analyzed in desorption ionization mass spectrometry of a substance to be measured. By applying, to a substance to be analyzed, an ionization assisting reagent including an organic acid including a functional group represented by —(CF2)COOH and having a boiling point of 150° C. or more, and a polyhydric alcohol having a melting point of 20° C. or less and a boiling point of 150° C. or more at normal pressure, the organic acid, which is a component of the ionization assisting reagent, effectively donates a proton to the substance to be analyzed, thereby improving ionization efficiency, and the polyhydric alcohol, which is a component of the ionization assisting reagent, inhibits fragmentation.
摘要翻译：本发明提供了一种能够在每个测量部位实现待分析物质的软电离和高电离效率的方法，而不会在待测物质的解吸电离质谱中损害待分析物质的二维分布状态。通过将被分析物质应用于包含由 - （CF 2）COOH表示的官能团和沸点为150℃以上的有机酸的电离助剂和具有150℃以上的沸点的多元醇在常压下为20℃以下，沸点为150℃以上，作为电离辅助剂的成分的有机酸能够有效地向待分析物质提供质子，从而提高离子化作为电离助剂的成分的多元醇抑制分解。

3. 发明申请

US20120181421A1 INFORMATION ACQUISITION METHOD 失效
标题翻译：信息获取方法
公开(公告)号：US20120181421A1
公开(公告)日：2012-07-19
申请号：US13351675
申请日：2012-01-17
申请人： Shuya Satoh , Hiroyuki Hashimoto
发明人： Shuya Satoh , Hiroyuki Hashimoto
IPC分类号： H01J49/26 , H01J49/40
CPC分类号： H01J49/142 , Y10T436/24 , Y10T436/25 , Y10T436/25875
摘要： Provided is a method that achieves both of soft ionization and high ionization efficiency of a substance to be analyzed at each measurement site without impairing a two-dimensional distribution state of the substance to be analyzed in desorption ionization mass spectrometry of a substance to be measured. By applying, to a substance to be analyzed, an ionization assisting reagent including an organic acid including a functional group represented by —(CF2)COOH and having a boiling point of 150° C. or more, and a polyhydric alcohol having a melting point of 20° C. or less and a boiling point of 150° C. or more at normal pressure, the organic acid, which is a component of the ionization assisting reagent, effectively donates a proton to the substance to be analyzed, thereby improving ionization efficiency, and the polyhydric alcohol, which is a component of the ionization assisting reagent, inhibits fragmentation.
摘要翻译：本发明提供了一种能够在每个测量部位实现待分析物质的软电离和高电离效率的方法，而不会在待测物质的解吸电离质谱中损害待分析物质的二维分布状态。通过将被分析物质应用于包含由 - （CF 2）COOH表示的官能团和沸点为150℃以上的有机酸的电离助剂和具有150℃以上的沸点的多元醇在常压下为20℃以下，沸点为150℃以上，作为电离辅助剂的成分的有机酸能够有效地向待分析物质提供质子，从而提高离子化作为电离助剂的成分的多元醇抑制分解。

4. 发明申请

US20120301968A1 MASS SPECTROMETRY METHOD 有权
标题翻译：质谱法
公开(公告)号：US20120301968A1
公开(公告)日：2012-11-29
申请号：US13576124
申请日：2011-03-28
申请人： Jumpei Naito , Shuya Satoh , Manabu Komatsu , Hiroyuki Hashimoto
发明人： Jumpei Naito , Shuya Satoh , Manabu Komatsu , Hiroyuki Hashimoto
IPC分类号： G01N27/62
CPC分类号： G01N33/6851 , H01J49/00 , Y10T436/182
摘要： The problem to be solved by the present invention is to provide a method for treating a polypeptide including a cysteine residue for enhancing the sensitivity of detection thereof in mass spectrometry using charged particles. A mass spectrometry method for a polypeptide including a cysteine residue is provided which includes treating the polypeptide including a cysteine residue with a cyanation agent, treating the cyanated polypeptide with a base, and then desorption ionizing the base-treated polypeptides using charged particles for mass spectrometry.
摘要翻译：本发明要解决的问题是提供一种用于在使用带电粒子的质谱法中治疗包含半胱氨酸残基的多肽以提高其检测灵敏度的方法。提供了包括半胱氨酸残基的多肽的质谱法，其包括用氰化剂处理包含半胱氨酸残基的多肽，用碱处理氰化多肽，然后使用带电粒子对基质处理的多肽进行解吸，用于质谱法。

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