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1. 发明申请

US20090280091A1 5-FLUOROURACIL-RESISTANT BACTERIA AND METHOD FOR PRODUCTION THEREOF 有权
标题翻译： 5-氟耐药细菌及其生产方法
公开(公告)号：US20090280091A1
公开(公告)日：2009-11-12
申请号：US11910880
申请日：2006-04-04
申请人： Yoshinori Hamaji , Minoru Fujimori , Jun Amano , Shun 'Ichiro Taniguchi
发明人： Yoshinori Hamaji , Minoru Fujimori , Jun Amano , Shun 'Ichiro Taniguchi
IPC分类号： A61K35/74 , C12N1/20 , A61P35/00
CPC分类号： C12N9/78 , C12N1/20
摘要： The present invention provides a method for producing a cytosine deaminase (CD)-expressing, 5-fluorouracil (5-FU)-resistant microorganism which can grow in anaerobic tumor tissues, can express CD, and has a resistance to 5-FU at a concentration that is at least effective for antitumor activity. More specifically, the method is a method (A) comprising performing subculture or acclimation culture of a CD-expressing microorganism which can grow in anaerobic tumor tissues, in the presence of 5-fluorocytosine (5-FC), or a method (B) comprising (1) performing subculture or acclimation culture of a microorganism which can grow in anaerobic tumor tissues and does not express CD, in the presence of 5-FU to produce a 5-FU-resistant microorganism and (2) transforming the 5-FU-resistant microorganism by introducing a CD gene. The present invention also provides the CD-expressing, 5-FU-resistant microorganism and a pharmaceutical composition comprising the microorganism.
摘要翻译：本发明提供了一种能够在厌氧性肿瘤组织中生长的胞嘧啶脱氨酶（CD）表达的5-氟尿嘧啶（5-FU）抗性微生物的制备方法，其可以表达CD，并且具有对5-FU的抗性至少对抗肿瘤活性有效的浓度。更具体地，该方法是包括在5-氟胞嘧啶（5-FC）或方法（B）的存在下进行可在厌氧性肿瘤组织中生长的表达CD的微生物的传代培养或适应培养的方法（A）包括（1）在5-FU存在下进行在厌氧性肿瘤组织中生长并且不表达CD的微生物的传代培养或适应培养以产生5-FU抗性微生物，和（2）转化5-FU 引入CD基因的抗性微生物。本发明还提供了表达CD的5-FU抗性微生物和包含微生物的药物组合物。

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