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    • 5. 发明授权
    • Nucleic acid amplification primers for detecting cytokeratins and examination method with the use of the primers
    • 用于检测细胞角蛋白的核酸扩增引物和使用引物的检查方法
    • US08076080B2
    • 2011-12-13
    • US10514776
    • 2003-05-20
    • Sachiyo TadaYasumasa AkaiYasuyuki ImuraShigeki AbeHarumi Minekawa
    • Sachiyo TadaYasumasa AkaiYasuyuki ImuraShigeki AbeHarumi Minekawa
    • C12Q1/68C12P19/34C07H21/04
    • C12Q1/6844C12Q1/686C12Q1/6886C12Q2600/112C12Q2531/119C12Q2527/101
    • It is intended to provide novel primers to be used in gene amplification reactions for detecting human cytokeratins (Human CK). A primer containing an oligonucleotide comprising a base sequence which is selected from among the the 270- to 1375-th regions of the base sequence represented by SEQ ID NO:1 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:2 to 341 is constructed. Further, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 270- to 930-th regions of the base sequence represented by SEQ ID NO:342 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:343 to 434 is constructed. Furthermore, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 340 to 490-th regions or the 495- to 1050-th regions of the base sequence represented by SEQ ID NO:435 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:436 to 474 is constructed.
    • 旨在提供用于检测人细胞角蛋白(Human CK)的基因扩增反应中使用的新引物。 一种含有寡核苷酸的引物,其包含选自SEQ ID NO:1所示的碱基序列的270〜1375位的碱基序列及其互补链的区域, 构建SEQ ID NOS:2至341。 此外,含有包含选自SEQ ID NO:342所示的碱基序列的270〜930区域的碱基序列的寡核苷酸的引物及其互补链的区域,例如任何 构建为SEQ ID NO:343〜434。 此外,含有选自SEQ ID NO:435表示的碱基序列的340〜490个区域或495〜1050个区域的碱基序列的寡核苷酸的引物及其互补链的区域 并且由例如SEQ ID NO:436至474中的任一个表示。
    • 9. 发明申请
    • METHOD AND KIT FOR DETERMINING THE AMOUNT OF DNA BINDING PROTEIN
    • 用于测定DNA结合蛋白质量的方法和试剂盒
    • US20090004749A1
    • 2009-01-01
    • US12163431
    • 2008-06-27
    • Koichi YamagataShigeki Abe
    • Koichi YamagataShigeki Abe
    • G01N33/00
    • G01N33/5308G01N33/6872
    • A kit for determining a quantity of a target DNA binding protein in a liquid sample by using a fluorescence correlation spectroscopy, comprising a first measuring reagent including a fluorescent-labeled nucleic acid probe and a first unlabeled nucleic acid probe, a second measuring reagent including the fluorescent-labeled nucleic acid probe and a second unlabeled nucleic acid probe, wherein the target DNA binding protein is capable of binding to the fluorescent-labeled nucleic acid probe and the first unlabeled nucleic acid probe, and is not capable of binding to the second unlabeled nucleic acid probe; and a method for determining a quantity of a target DNA binding protein in a liquid sample is also disclosed.
    • 一种用于通过使用荧光相关光谱测定液体样品中的靶DNA结合蛋白的量的试剂盒,其包括含有荧光标记的核酸探针和第一未标记的核酸探针的第一测定试剂,包含第 荧光标记的核酸探针和第二个未标记的核酸探针,其中靶DNA结合蛋白能够结合荧光标记的核酸探针和第一个未标记的核酸探针,并且不能结合第二个未标记的核酸探针 核酸探针; 还公开了一种用于测定液体样品中目标DNA结合蛋白的量的方法。