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1. 发明授权

US07135101B2 Cassette assembly for electrophoresis gels 有权
公开(公告)号：US07135101B2
公开(公告)日：2006-11-14
申请号：US10000473
申请日：2001-10-24
申请人： Shaun Atchison , Jens Sommer-Knudsen , Nicola Boyd , Des Berry
发明人： Shaun Atchison , Jens Sommer-Knudsen , Nicola Boyd , Des Berry
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44704
摘要： This invention is directed to a cassette for electrophoresis gels comprising: first and second planar wall members having inner and outer surfaces, top and bottom edges, and lateral edges wherein the wall members are oriented generally parallel to each other and such that the inner wall of each wall member is proximate to the inner wall of the other wall member; spacing means disposed between the inner walls of the wall members and adapted to provide a space for an electrophoresis gel between the inner walls of the wall members, wherein the cassette has an interior which is defined by the space between the wall members; and locking means adapted to prevent locking engagement of the members unless the inner surfaces of the wall members and are substantially parallel to each other and are separated by a predetermined distance, and when the wall members are in locked engagement, to substantially prevent any movement of the wall members away from such locked engagement.

2. 发明授权

US06878257B2 Cassette for electrophoretic gels 失效
标题翻译：胶片用于电泳凝胶
公开(公告)号：US06878257B2
公开(公告)日：2005-04-12
申请号：US10167540
申请日：2002-06-11
申请人： Howard Pericles Manusu , Shaun Atchison
发明人： Howard Pericles Manusu , Shaun Atchison
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44743 , G01N27/44704
摘要： An improved cassette for use in the formation of an electrophoretic gel comprises two plates with substantially planar walls having two sides and two ends so arranged in a side by side spaced apart array to form a gel receiving space between them. A plurality of dividing ribs on one or each of the walls extend from a first end of the wall or walls substantially parallel to at least one of the sides thereof to a rib base end. The ribs extend into the space so as to subdivide at least one end of the space into a plurality of substantially parallel wells. A plurality of holes extend through at least one of the walls of the cassette located at or adjacent the base of the dividing rib and aligned with the rib. The arrangement of holes allow sufficient current flow to replace that lost due to the dividing ribs. The structure retains the advantage of having solid dividing walls which are considerably more resilient than dividing walls made from fingers of gel even when reinforced with pegs or projections and yet by virtue of the provision of a hole at the base of the ribs allows sufficient current flow at the base of each rib that the current flow through the space is substantially uniform, thus preventing spreading of the bands as they form in the body of the gel.
摘要翻译：用于形成电泳凝胶的改进盒包括两个板，其具有基本平坦的壁，其具有两侧和两端，并排设置成间隔开的阵列，以在它们之间形成凝胶接收空间。在一个或每个壁上的多个分隔肋从基本平行于其至少一侧的壁或壁的第一端延伸到肋基端。肋延伸到空间中，以便将空间的至少一端细分成多个基本平行的孔。多个孔延伸穿过位于分隔肋的底部或邻近分隔肋的基部并与肋对准的盒的至少一个壁。孔的布置允许足够的电流来代替由于分隔肋而损失的电流。该结构保留了具有实心分隔壁的优点，即，即使用钉或突起来增强，由具有由凝胶指状物制成的分隔壁也具有显着更大的弹性，并且由于在肋的底部设置孔足够的电流在每个肋的基部，电流流经该空间的基本上是均匀的，从而防止当它们在凝胶体中形成时扩散。

3. 发明授权

US06413402B1 Cassette for electrophoretic gels 失效
标题翻译：胶片用于电泳凝胶
公开(公告)号：US06413402B1
公开(公告)日：2002-07-02
申请号：US09543855
申请日：2000-04-05
申请人： Howard Pericles Manusu , Shaun Atchison
发明人： Howard Pericles Manusu , Shaun Atchison
IPC分类号： G01N2726
CPC分类号： G01N27/44743 , G01N27/44704
摘要： An improved cassette for use in the formation of an electrophoretic gel comprises two plates with substantially planar walls having two sides and two ends so arranged in a side by side spaced apart array to form a gel receiving space between them. A plurality of dividing ribs on one or each of the walls extend from a first end of the wall or walls substantially parallel to at least one of the sides thereof to a rib base end. The ribs extend into the space so as to subdivide at least one end of the space into a plurality of substantially parallel wells. A plurality of holes extend through at least one of the walls of the cassette located at or adjacent the base of the dividing rib and aligned with the rib. The arrangement of holes allow sufficient current flow to replace that lost due to the dividing ribs. The structure retains the advantage of having solid dividing walls which are considerably more resilient than dividing walls made from fingers of gel even when reinforced with pegs or projections, and yet by virtue of the provision of a hole at the base of the ribs allows sufficient current flow at the base of each rib that the current flow through the space is substantially uniform, thus preventing spreading of the bands as they form in the body of the gel.
摘要翻译：用于形成电泳凝胶的改进盒包括两个板，其具有基本平坦的壁，其具有两侧和两端，并排设置成间隔开的阵列，以在它们之间形成凝胶容纳空间。在一个或每个壁上的多个分隔肋从基本平行于其至少一侧的壁或壁的第一端延伸到肋基端。肋延伸到空间中，以便将空间的至少一端细分成多个基本平行的孔。多个孔延伸穿过位于分隔肋的底部或邻近分隔肋的基部并与肋对准的盒的至少一个壁。孔的布置允许足够的电流来代替由于分隔肋而损失的电流。该结构保留了具有实心分隔壁的优点，即，即使用钉或突起来加强，由分离壁制成的分隔壁也具有更大的弹性，并且由于在肋的底部设置孔足够的电流在每个肋的基部流动，电流流经该空间的基本上是均匀的，从而防止在凝胶体形成时带的扩散。

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