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1. 发明申请

US20110027847A1 HEXOSE-PENTOSE COFERMENTING YEAST HAVING EXCELLENT XYLOSE FERMENTABILITY AND METHOD FOR HIGHLY EFFICIENTLY PRODUCING ETHANOL USING THE SAME 有权
标题翻译：具有优异的XYLOSE发酵性的HEXOSE-PENTOSE COFERMENE YEAST和使用其高效生产乙醇的方法
公开(公告)号：US20110027847A1
公开(公告)日：2011-02-03
申请号：US12864360
申请日：2009-01-22
申请人： Akinori Matsushika , Shigeki Sawayama , Hiroyuki Inoue , Keisuke Makino , Tsutomu Kodaki , Seiya Watanabe
发明人： Akinori Matsushika , Shigeki Sawayama , Hiroyuki Inoue , Keisuke Makino , Tsutomu Kodaki , Seiya Watanabe
IPC分类号： C12P7/06 , C12N1/19
CPC分类号： C12N15/81 , C12P7/06 , Y02E50/17
摘要： Genetic recombinant yeast expressing xylose reductase (XR), (wild-type or mutant) xylitol dehydrogenase (XDH), and xylulokinase (XK) and a method for highly efficiently producing ethanol from xylose using the yeast are provided. Pichia stipitis-derived XR and (wild-type or modified-type) XDH genes and Saccharomyces cerevisiae-derived XK gene were introduced via chromosomal integration. Thus, a genetic recombinant yeast having a high xylose fermentation rate, being capable of producing ethanol from xylose in high yields, and having high xylose fermentability in the presence of glucose, as well as a method using the recombinant yeast for highly efficiently producing ethanol from xylose or a saccharified solution from lignocellulose-based biomass are provided. Furthermore, a method for improving the xylose fermentability of the genetic recombinant yeast of the present invention via acclimatization treatment is also provided herein.
摘要翻译：提供表达木糖还原酶（XR），（野生型或突变体）木糖醇脱氢酶（XDH）和木酮糖激酶（XK）的遗传重组酵母和使用酵母从木糖高效生产乙醇的方法。通过染色体整合引入派生芽孢杆菌衍生的XR和（野生型或修饰型）XDH基因和酿酒酵母衍生的XK基因。因此，具有高木糖发酵速率的基因重组酵母，能够以高产率从木糖产生乙醇，并且在葡萄糖存在下具有高木糖发酵能力，以及使用重组酵母高效生产乙醇的方法提供木糖或基于木质纤维素的生物质的糖化溶液。此外，还提供了通过驯化处理改善本发明的遗传重组酵母的木糖发酵能力的方法。

2. 发明授权

US08445243B2 Hexose-pentose cofermenting yeast having excellent xylose fermentability and method for highly efficiently producing ethanol using the same 有权
标题翻译：具有优异的木糖发酵能力的己糖 - 戊糖共发酵酵母和使用其的高效生产乙醇的方法
公开(公告)号：US08445243B2
公开(公告)日：2013-05-21
申请号：US12864360
申请日：2009-01-22
申请人： Akinori Matsushika , Shigeki Sawayama , Hiroyuki Inoue , Keisuke Makino , Tsutomu Kodaki , Seiya Watanabe
发明人： Akinori Matsushika , Shigeki Sawayama , Hiroyuki Inoue , Keisuke Makino , Tsutomu Kodaki , Seiya Watanabe
IPC分类号： C12P7/06 , C12N1/14
CPC分类号： C12N15/81 , C12P7/06 , Y02E50/17
摘要： Genetic recombinant yeast expressing xylose reductase (XR), (wild-type or mutant) xylitol dehydrogenase (XDH), and xylulokinase (XK) and a method for highly efficiently producing ethanol from xylose using the yeast are provided. Pichia stipitis-derived XR and (wild-type or modified-type) XDH genes and Saccharomyces cerevisiae-derived XK gene were introduced via chromosomal integration. Thus, a genetic recombinant yeast having a high xylose fermentation rate, being capable of producing ethanol from xylose in high yields, and having high xylose fermentability in the presence of glucose, as well as a method using the recombinant yeast for highly efficiently producing ethanol from xylose or a saccharified solution from lignocellulose-based biomass are provided. Furthermore, a method for improving the xylose fermentability of the genetic recombinant yeast of the present invention via acclimatization treatment is also provided herein.
摘要翻译：提供表达木糖还原酶（XR），（野生型或突变体）木糖醇脱氢酶（XDH）和木酮糖激酶（XK）的遗传重组酵母和使用酵母从木糖高效生产乙醇的方法。通过染色体整合引入派生芽孢杆菌衍生的XR和（野生型或修饰型）XDH基因和酿酒酵母衍生的XK基因。因此，具有高木糖发酵速率的基因重组酵母，能够以高产率从木糖产生乙醇，并且在葡萄糖存在下具有高木糖发酵能力，以及使用重组酵母高效生产乙醇的方法提供木糖或基于木质纤维素的生物质的糖化溶液。此外，还提供了通过驯化处理改善本发明的遗传重组酵母的木糖发酵能力的方法。

3. 发明申请

US20080070795A1 LINKER COMPOUND, PROBE, AND PROBE-IMMOBILIZED CARRIER 审中-公开
标题翻译：链接化合物，探针和探针固定载体
公开(公告)号：US20080070795A1
公开(公告)日：2008-03-20
申请号：US11851865
申请日：2007-09-07
申请人： Keisuke Makino , Tsutomu Kodaki , Seiya Watanabe , Seung Pack , Mitsuru Nonogawa , Hiroo Wada , Yasuko Yoshida , Kazunari Yamada
发明人： Keisuke Makino , Tsutomu Kodaki , Seiya Watanabe , Seung Pack , Mitsuru Nonogawa , Hiroo Wada , Yasuko Yoshida , Kazunari Yamada
IPC分类号： C40B30/06 , C07H21/04 , C40B40/08
CPC分类号： C07D498/04 , C07H19/24 , C07H21/04
摘要： The present invention aims to provide a technique for stably immobilizing a probe on a carrier The present invention provides a linker compound represented by formula (1): wherein A represents a hydrogen atom, another substituent, a sugar, or a derivative thereof.
摘要翻译：本发明的目的在于提供一种用于将探针稳定地固定在载体上的技术。本发明提供由式（1）表示的连接剂化合物：其中A表示氢原子，另一取代基，糖或其衍生物。

4. 发明申请

US20120270290A1 PENTOSE TRANSPORTER 审中-公开
公开(公告)号：US20120270290A1
公开(公告)日：2012-10-25
申请号：US13393664
申请日：2010-09-01
申请人： Seiya Watanabe , Shigeki Sawayama
发明人： Seiya Watanabe , Shigeki Sawayama
IPC分类号： C12P7/10 , C12P7/06 , C07K14/00 , C07H21/04
CPC分类号： C12P7/10 , C07K14/39 , Y02E50/16 , Y02E50/17
摘要： Disclosed is the production of useful substances such as bioethanol from pentose through effective use of biomass resources. Specifically, the invention discloses the following: Use of HGT2 gene or an expression product protein thereof as a xylose transporter. A xylose and/or L-arabinose transporter as an expression product protein of a gene selected from the group consisting of HGT2 gene, XUT1 gene, and HXT2.4 gene. Use of a gene selected from the group consisting of HGT2 gene, XUT1 gene, and HXT2.4 gene as a xylose and/or L-arabinose transporter. A method for producing bioethanol, the method comprising introducing into yeast at least one gene selected from the group consisting of HGT2 gene, XUT1 gene, and HXT2.4 gene, and culturing the yeast in the presence of a biomass that contains xylose and/or L-arabinose.
摘要翻译：公开了通过有效利用生物质资源从戊糖生产有用物质如生物乙醇。具体地，本发明公开了以下：HGT2基因或其表达产物蛋白质作为木糖转运体的用途。作为选自HGT2基因，XUT1基因和HXT2.4基因的基因的表达产物蛋白质的木糖和/或L-阿拉伯糖转运蛋白。使用选自HGT2基因，XUT1基因和HXT2.4基因的基因作为木糖和/或L-阿拉伯糖转运蛋白。一种生产生物乙醇的方法，所述方法包括向酵母中导入至少一种选自HGT2基因，XUT1基因和HXT2.4基因的基因，并在含有木糖和/或 L-阿拉伯糖。

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