专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5976857A Cross-linked polypeptide fragments of .beta.-galactosidase 失效
标题翻译： β-半乳糖苷酶的交联多肽片段
公开(公告)号：US5976857A
公开(公告)日：1999-11-02
申请号：US592013
申请日：1996-01-26
申请人： Michael J. Powell , Pyare Khanna , Scott J. Eisenbeis , David Lingenfelter , Lutz F. Tietze
发明人： Michael J. Powell , Pyare Khanna , Scott J. Eisenbeis , David Lingenfelter , Lutz F. Tietze
IPC分类号： C12N9/38 , C12Q1/34 , C12Q1/37 , C12Q1/42 , C12Q1/44 , G01N1/48 , A61K38/00 , C12N9/96
CPC分类号： C12Y302/01023 , C12N9/2471
摘要： Formation of an intramolecular cross-link in enzyme donor polypeptide fragments of .beta.-galactosidase, thereby forming a cyclic enzyme donor which is hindered from complementation with an enzyme acceptor fragment to form active of .beta.-galactosidase. The cyclic enzyme donor can be linearized by cleaving to restore complementation ability. Assays in which such cyclic enzyme donors are linearized by specific analytes are disclosed, as well as novel homobifunctional bis-maleimido cross-linking agents of the formula ##STR1## wherein R is hydroxy or acetate.
摘要翻译：在β-半乳糖苷酶的酶供体多肽片段中形成分子内交联，从而形成环状酶供体，其受到酶受体片段的互补阻碍形成β-半乳糖苷酶的活性。循环酶供体可以通过切割线性化以恢复互补能力。公开了这样的环状酶供体通过特异性分析物线性化的测定，以及下式的新型双官能双马来酰亚胺交联剂，其中R是羟基或乙酸酯。

2. 发明授权

US5492813A Muteins of .beta.-galactosidase fragments having increased activity 失效
标题翻译： β-半乳糖苷酶片段的突变蛋白具有增加的活性
公开(公告)号：US5492813A
公开(公告)日：1996-02-20
申请号：US146673
申请日：1993-11-01
申请人： Scott J. Eisenbeis , Sophie J. Boguslawski , Mark Krevolin , David J. Ledden
发明人： Scott J. Eisenbeis , Sophie J. Boguslawski , Mark Krevolin , David J. Ledden
IPC分类号： C12N9/38 , C12N15/09 , C12R1/19 , G01N33/535 , G01N33/542 , C12N15/56
CPC分类号： C12Y302/01023 , C12N9/2471 , G01N33/535 , Y10S435/963
摘要： Muteins of enzyme acceptor polypeptide fragments of .beta.-galactosidase are provided which exhibit substantially increased kinetic complementation activity with no significant loss in stability. A preferred enzyme acceptor fragment has an amino acid other than cysteine located at position 500 of the natural sequence. An especially preferred substitution is serine or valine. Other preferred muteins have an amino acid other than methionine located at position 443, with leucine being especially preferred, or an amino acid other than cysteine at position 76, with leucine being an especially preferred substitution. Also provided are methods for producing the novel muteins, reagent compositions comprising the novel muteins, and immunoassay methods for determining an analyte in which the novel mutein recombines with an enzyme donor polypeptide fragment to form enzymatically active .beta.-galactosidase.
摘要翻译：提供了β-半乳糖苷酶的酶受体多肽片段的突变蛋白，其表现出显着增加的动力学互补活性，而没有显着的稳定性损失。优选的酶受体片段具有位于天然序列的位置500处的半胱氨酸以外的氨基酸。特别优选的取代是丝氨酸或缬氨酸。其他优选的突变蛋白具有除位于第443位的甲硫氨酸以外的氨基酸，特别优选亮氨酸，或位置76处的半胱氨酸以外的氨基酸，亮氨酸是特别优选的取代基。还提供了用于产生新型突变蛋白的方法，包含新颖突变蛋白的试剂组合物和用于测定其中新型突变蛋白与酶供体多肽片段重组以形成酶活性β-半乳糖苷酶的分析物的免疫测定方法。

3. 发明授权

US5763196A Assays using cross-linked polypeptide fragments of .beta.-galactosidase 失效
标题翻译：使用β-半乳糖苷酶的交联多肽片段进行测定
公开(公告)号：US5763196A
公开(公告)日：1998-06-09
申请号：US592029
申请日：1996-01-26
申请人： Michael J. Powell , Pyare Khanna , David Lingenfelter , Scott J. Eisenbeis
发明人： Michael J. Powell , Pyare Khanna , David Lingenfelter , Scott J. Eisenbeis
IPC分类号： C12Q1/37 , G01N33/53 , C12Q1/34
CPC分类号： C12Q1/37
摘要： Formation of an intramolecular cross-link in enzyme donor polypeptide fragments of .beta.-galactosidase, thereby forming a cyclic enzyme donor which is hindered from complementation with an enzyme acceptor fragment to form active of .beta.-galactosidase. The cyclic enzyme donor can be linearized by cleaving to restore complementation ability. Assays in which such cyclic enzyme donors are linearized by specific analytes are disclosed, as well as novel homobifunctional bis-maleimido cross-linking agents of the formula ##STR1## wherein R is hydroxy or acetate.
摘要翻译：在β-半乳糖苷酶的酶供体多肽片段中形成分子内交联，从而形成环状酶供体，其受到酶受体片段的互补阻碍形成β-半乳糖苷酶的活性。循环酶供体可以通过切割线性化以恢复互补能力。公开了其中这种环状酶供体由特异性分析物线性化的测定，以及式（IMAGE）其中R是羟基或乙酸酯的新型双官能双马来酰亚胺交联剂。

4. 发明授权

US5464747A Oxidation-resistant muteins of .beta.-galactosidase fragments 失效
标题翻译： β-半乳糖苷酶片段的抗氧化突变蛋白
公开(公告)号：US5464747A
公开(公告)日：1995-11-07
申请号：US146633
申请日：1993-10-29
申请人： Scott J. Eisenbeis , Mark Krevolin , Christopher P. Bryant , Sophie J. Boguslawski , David J. Ledden , Scott Clark
发明人： Scott J. Eisenbeis , Mark Krevolin , Christopher P. Bryant , Sophie J. Boguslawski , David J. Ledden , Scott Clark
IPC分类号： G01N33/536 , C12N9/38 , C12N15/09 , C12Q1/34 , C12R1/19 , G01N33/542 , G01N33/573 , C12N15/56 , G01N33/535
CPC分类号： C12Y302/01023 , C12N9/2471 , G01N33/573 , Y10S435/963
摘要： A mutein of an enzyme acceptor polypeptide fragment of .beta.-galactosidase which is resistant to oxidation is provided. The enzyme acceptor fragment has an amino acid other than cysteine located at position 602 of the natural sequence. An especially preferred substitution is serine. Also provided are a method for producing the novel mutein, a reagent composition comprising the novel mutein, and an immunoassay method for determining an analyte in which the novel mutein recombines with an enzyme donor polypeptide fragment to form enzymatically active .beta.-galactosidase.
摘要翻译：提供耐氧化的β-半乳糖苷酶的酶受体多肽片段的突变蛋白。酶受体片段具有位于天然序列602位的半胱氨酸以外的氨基酸。特别优选的取代是丝氨酸。还提供了用于产生新型突变蛋白的方法，包含新型突变蛋白的试剂组合物和用于测定其中新型突变蛋白与酶供体多肽片段重组以形成酶活性β-半乳糖苷酶的分析物的免疫测定方法。

5. 发明授权

US5795718A Detection of complementary nucleotide sequences 失效
标题翻译：检测互补核苷酸序列
公开(公告)号：US5795718A
公开(公告)日：1998-08-18
申请号：US453971
申请日：1995-05-30
申请人： Scott J. Eisenbeis
发明人： Scott J. Eisenbeis
IPC分类号： C12Q1/34 , C12Q1/68 , C12Q1/70 , C07H21/04 , C07H19/04 , C07H21/00
CPC分类号： C12Q1/6816
摘要： The invention relates to a method for detection of a specific nucleic acid sequence which comprises forming a reaction mixture by combining (1) a sample suspected of containing a nucleic acid; (2) a probe/enzyme donor polypeptide conjugate comprising (a) an enzyme donor polypeptide sequence comprising a .beta.-galactosidase fragment; and (b) a single-stranded oligonucleotide sequence attached to (a) and capable of hybridizing with said nucleic acid; (3) an enzyme acceptor polypeptide capable of forming an active enzyme upon complementation with said enzyme donor fragment; and (4) a substrate for .beta.-galactosidase; and detecting hybridization of said probe/enzyme donor conjugate to said sample nucleic acid to form a double strand-specific sequence by determining the amount or rate of enzyme activity on said substrate in said reaction mixture. The method can also include a "proof reading" function by incubating the hybridized probe with at least one double-strand-specific, sequence-specific restriction endonuclease. Novel kits for use in carrying out the method are also included.
摘要翻译：本发明涉及检测特异性核酸序列的方法，其包括通过组合（1）怀疑含有核酸的样品形成反应混合物; （2）探针/酶供体多肽缀合物，其包含（a）包含β-半乳糖苷酶片段的酶供体多肽序列; 和（b）与（a）连接并能够与所述核酸杂交的单链寡核苷酸序列; （3）能够在与所述酶供体片段互补时能够形成活性酶的酶受体多肽; 和（4）β-半乳糖苷酶的底物; 以及通过测定所述反应混合物中所述底物上的酶活性的量或速率来检测所述探针/酶供体缀合物与所述样品核酸的杂交以形成双链特异性序列。该方法还可以通过将杂交探针与至少一个双链特异性序列特异性限制性内切核酸酶孵育来包括“证明读数”功能。还包括用于实施该方法的新型试剂盒。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式