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1. 发明申请

US20110104710A1 CONSTRUCTION OF EXPRESSION SYSTEM FOR RNA POLYMERASE DERIVED FROM INFLUENZA VIRUS, CRYSTALLIZATION OF THE RNA POLYMERASE, AND SCREENING METHOD FOR ANTI-INFLUENZA AGENT 有权
标题翻译：来自流感病毒的RNA聚合酶的表达系统的构建，RNA聚合酶的结晶和用于抗流感病毒的筛选方法
公开(公告)号：US20110104710A1
公开(公告)日：2011-05-05
申请号：US13001091
申请日：2009-07-02
申请人： Kyosuke Nagata , Atsushi Kawaguchi , Sam-Yong Park , Eiji Obayashi
发明人： Kyosuke Nagata , Atsushi Kawaguchi , Sam-Yong Park , Eiji Obayashi
IPC分类号： G01N33/53 , C07K14/00 , C12N15/63 , C12N5/10 , C12P21/02 , C07H21/00
CPC分类号： C07K14/005 , C07K2299/00 , C12N9/127 , C12N2760/16122
摘要： The present invention aims to express influenza virus RNA polymerase on a large scale, to crystallize the influenza virus RNA polymerase, and to provide a method for screening a substance capable of serving as an active ingredient in anti-influenza drugs which target a protein highly conserved among influenza virus species.The present invention provides a complex comprising a polypeptide consisting of an amino acid sequence at positions 239-716 of the RNA polymerase PA subunit in influenza A/Puerto Rico/8/1934 H1N1 and a polypeptide consisting of an amino acid sequence at positions 1-81 of the RNA polymerase PB1 subunit in influenza A/Puerto Rico/8/1934 H1N1, as well as a method for screening a substance capable of serving as an active ingredient in anti-influenza drugs, which comprises the step of selecting a substance which inhibits the interaction between α-subunit and β-subunit 1, each constituting influenza virus RNA polymerase, in the presence of a candidate substance.
摘要翻译：本发明旨在大规模表达流感病毒RNA聚合酶，以使流感病毒RNA聚合酶结晶，并提供一种筛选能够作为靶向高度保守的蛋白质的抗流感药物中的活性成分的物质的方法流感病毒。本发明提供了一种复合物，其包含由流感A /波多黎各/ 8/1934型H1N1中的RNA聚合酶PA亚单位的第239-716位的氨基酸序列组成的多肽，以及由1-位的氨基酸序列组成的多肽， 81流感病毒A /波多黎各/ 8/1934型H1N1中的RNA聚合酶PB1亚基，以及用于筛选能够用作抗流感药物中的活性成分的物质的方法，其包括以下步骤：在候选物质的存在下，抑制各自构成流感病毒RNA聚合酶的α-亚基和b-b亚单位之间的相互作用。

2. 发明授权

US5818653A Multiple-values digital signal magnetic recording and reproducing apparatus using a QAM and PSK modulator, pilot signal and a viterbi decoder 失效
标题翻译：使用QAM和PSK调制器的多值数字信号磁记录和再现装置，导频信号和维特比解码器
公开(公告)号：US5818653A
公开(公告)日：1998-10-06
申请号：US785166
申请日：1997-01-13
申请人： Sam-yong Park , Yong-deok Chang
发明人： Sam-yong Park , Yong-deok Chang
IPC分类号： G11B5/00 , G11B5/09 , G11B20/10 , G11B20/14 , G11B20/18 , H04L27/36 , H04L27/38 , H04N5/91 , H04N5/92 , H04N5/922 , H04N19/423 , H04N19/60 , H04N19/70 , H04N19/80 , H04N19/85
CPC分类号： G11B20/10 , G11B20/1496 , G11B20/1833
摘要： A digital signal magnetic recording and reproducing apparatus includes an encoding portion for convolutionally coding an input digital data, a modulating portion for quadrature-amplitude-modulating and phase-shift-keying-modulating the convolutionally coded digital data, a pilot signal mixer for generating a pilot signal and adding it to the modulated signal, and a recording controller for forming a magnetized signal suitable for a magnetic recording medium from the modulated signal mixed with the pilot signal. The apparatus also includes a reproducing controller for outputting a digital signal, in which distortion and deterioration produced during transmission are corrected, from the magnetized signal reproduced in the magnetic recording medium, a carrier wave restoring portion for generating a clock signal at the carrier signal frequency in response to a pilot signal in the reproduced magnetized signal, a demodulating portion for demodulating the quadrature-amplitude-modulated signal output from the reproducing controller and a decoding portion for outputting original digital data by Viterbi-decoding convolutionally coded data from the demodulating portion. The apparatus increases frequency utilization efficiency by adopting a multiple valued digital modulation method, which is effective in recording in a high density signal. The apparatus is characterized in that the overall signal processing is processed digitally.
摘要翻译：一种数字信号磁记录和再现装置包括用于对输入数字数据进行卷积编码的编码部分，用于正交幅度调制的调制部分和对卷积编码数字数据进行相移键控调制的导频信号混合器，用于产生导频信号并将其添加到调制信号，以及记录控制器，用于根据与导频信号混合的调制信号形成适合于磁记录介质的磁化信号。该装置还包括：再现控制器，用于输出在磁记录介质中再现的磁化信号中校正发送期间产生的失真和劣化的数字信号;载波恢复部分，用于以载波信号频率产生时钟信号响应于再现的磁化信号中的导频信号，解调部分，用于解调从再现控制器输出的正交幅度调制信号和解码部分，用于通过维特比解码来自解调部分的卷积编码数据输出原始数字数据。该装置采用多值数字调制方式提高了频率利用效率，该方法在高密度信号记录中是有效的。该装置的特征在于，数字地处理整体信号处理。

3. 发明授权

US08569016B2 Construction and crystallization of expression system for RNA polymerase PB1-PB2 protein derived from influenza virus 有权
标题翻译：来自流感病毒的RNA聚合酶PB1-PB2蛋白的表达系统的构建和结晶
公开(公告)号：US08569016B2
公开(公告)日：2013-10-29
申请号：US13124581
申请日：2009-10-16
申请人： Eiji Obayashi , Sam-Yong Park , Kyosuke Nagata , Atsushi Kawaguchi
发明人： Eiji Obayashi , Sam-Yong Park , Kyosuke Nagata , Atsushi Kawaguchi
IPC分类号： C12P21/06 , G01N33/48 , C12N1/20 , C07K14/00
CPC分类号： C07K14/005 , C07K2299/00 , C12N9/127 , C12N2760/16122 , G01N33/573 , G01N2333/11 , G01N2333/91275 , G01N2500/02
摘要： The present invention aims to express influenza virus RNA polymerase on a large scale, to crystallize the influenza virus RNA polymerase, and to provide a method for screening a substance capable of serving as an active ingredient in anti-influenza drugs.The present invention provides a complex comprising a polypeptide consisting of an amino acid sequence at positions 678-757 of the RNA polymerase PB1 subunit in influenza A/Puerto Rico/8/34 H1N1 (SEQ ID NO: 2) and a polypeptide consisting of an amino acid sequence at positions 1-37 of RNA polymerase PB2 subunit in influenza A/Puerto Rico/8/34 H1N1 (SEQ ID NO: 4). This complex can be crystallized in the presence of a precipitant such as potassium phosphate and PEG4000. Moreover, with the use of information on the crystal structure of this complex, it is possible to provide a method for screening a substance capable of serving as an active ingredient in anti-influenza drugs.
摘要翻译：本发明旨在大规模地表达流感病毒RNA聚合酶，以使流感病毒RNA聚合酶结晶，并提供一种筛选能够作为抗流感药物中的活性成分的物质的方法。本发明提供了一种复合物，其包含由流感A /波多黎各/ 8/34 H1N1（SEQ ID NO：2）中的RNA聚合酶PB1亚基的678-757位的氨基酸序列组成的多肽和由流感A /波多黎各/ 8/34 H1N1（SEQ ID NO：4）中RNA聚合酶PB2亚单位1-37位的氨基酸序列。该络合物可以在沉淀剂例如磷酸钾和PEG4000的存在下结晶。此外，通过使用关于该复合物的晶体结构的信息，可以提供一种筛选能够用作抗流感药物中的活性成分的物质的方法。

4. 发明授权

US08455229B2 RNA polymerase derived from influenza virus 有权
标题翻译：源自流感病毒的RNA聚合酶
公开(公告)号：US08455229B2
公开(公告)日：2013-06-04
申请号：US13001091
申请日：2009-07-02
申请人： Kyosuke Nagata , Atsushi Kawaguchi , Sam-Yong Park , Eiji Obayashi
发明人： Kyosuke Nagata , Atsushi Kawaguchi , Sam-Yong Park , Eiji Obayashi
IPC分类号： C12N9/00
CPC分类号： C07K14/005 , C07K2299/00 , C12N9/127 , C12N2760/16122
摘要： The present invention aims to express influenza virus RNA polymerase on a large scale, to crystallize the influenza virus RNA polymerase, and to provide a method for screening a substance capable of serving as an active ingredient in anti-influenza drugs which target a protein highly conserved among influenza virus species.The present invention provides a complex comprising a polypeptide consisting of an amino acid sequence at positions 239-716 of the RNA polymerase PA subunit in influenza A/Puerto Rico/8/1934 H1N1 and a polypeptide consisting of an amino acid sequence at positions 1-81 of the RNA polymerase PB1 subunit in influenza A/Puerto Rico/8/1934 H1N1, as well as a method for screening a substance capable of serving as an active ingredient in anti-influenza drugs, which comprises the step of selecting a substance which inhibits the interaction between α-subunit and β-subunit 1, each constituting influenza virus RNA polymerase, in the presence of a candidate substance.
摘要翻译：本发明旨在大规模表达流感病毒RNA聚合酶，以使流感病毒RNA聚合酶结晶，并提供一种筛选能够作为靶向高度保守的蛋白质的抗流感药物中的活性成分的物质的方法流感病毒。本发明提供了一种复合物，其包含由流感A /波多黎各/ 8/1934型H1N1中的RNA聚合酶PA亚单位的第239-716位的氨基酸序列组成的多肽，以及由1-位的氨基酸序列组成的多肽， 81流感病毒A /波多黎各/ 8/1934型H1N1中的RNA聚合酶PB1亚基，以及用于筛选能够用作抗流感药物中的活性成分的物质的方法，其包括以下步骤：在候选物质的存在下，抑制各自构成流感病毒RNA聚合酶的α-亚基和β-亚单位1之间的相互作用。

5. 发明申请

US20110262944A1 CONSTRUCTION AND CRYSTALLIZATION OF EXPRESSION SYSTEM FOR RNA POLYMERASE PB1-PB2 PROTEIN DERIVED FROM INFLUENZA VIRUS 有权
标题翻译：来自流感病毒的RNA聚合酶PB1-PB2蛋白的表达系统的构建和结晶
公开(公告)号：US20110262944A1
公开(公告)日：2011-10-27
申请号：US13124581
申请日：2009-10-16
申请人： Eiji Obayashi , Sam-Yong Park , Kyosuke Nagata , Atsushi Kawaguchi
发明人： Eiji Obayashi , Sam-Yong Park , Kyosuke Nagata , Atsushi Kawaguchi
IPC分类号： C12Q1/48 , C12N5/10 , C12N1/21 , C07H21/04 , C12N15/63 , C07K14/11 , C07K16/08 , C07K1/32 , C12P21/06 , C12N1/19
CPC分类号： C07K14/005 , C07K2299/00 , C12N9/127 , C12N2760/16122 , G01N33/573 , G01N2333/11 , G01N2333/91275 , G01N2500/02
摘要： The present invention aims to express influenza virus RNA polymerase on a large scale, to crystallize the influenza virus RNA polymerase, and to provide a method for screening a substance capable of serving as an active ingredient in anti-influenza drugs.The present invention provides a complex comprising a polypeptide consisting of an amino acid sequence at positions 678-757 of the RNA polymerase PB1 subunit in influenza A/Puerto Rico/8/34 H1N1 and a polypeptide consisting of an amino acid sequence at positions 1-37 of RNA polymerase PB2 subunit in influenza A/Puerto Rico/8/34 H1N1. This complex can be crystallized in the presence of a precipitant such as potassium phosphate and PEG4000. Moreover, with the use of information on the crystal structure of this complex, it is possible to provide a method for screening a substance capable of serving as an active ingredient in anti-influenza drugs.
摘要翻译：本发明旨在大规模地表达流感病毒RNA聚合酶，以使流感病毒RNA聚合酶结晶，并提供一种筛选能够作为抗流感药物中的活性成分的物质的方法。本发明提供了一种复合物，其包含由流感A /波多黎各/ 8/34 / H1N1中的RNA聚合酶PB1亚基的678-757位的氨基酸序列组成的多肽，以及由1-位的氨基酸序列组成的多肽， 37流感病毒A /波多黎各/ 8/34 H1N1 RNA聚合酶PB2亚基。该络合物可以在沉淀剂例如磷酸钾和PEG4000的存在下结晶。此外，通过使用关于该复合物的晶体结构的信息，可以提供一种筛选能够用作抗流感药物中的活性成分的物质的方法。

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