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1. 发明授权

US08304195B2 Antibody complexes and methods for immunolabeling 有权
标题翻译：抗体复合物和免疫标记方法
公开(公告)号：US08304195B2
公开(公告)日：2012-11-06
申请号：US12047131
申请日：2008-03-12
申请人： David Hagen , Joseph Beechem , Richard Haugland , Robert Archer , Rosaria Haugland
发明人： David Hagen , Joseph Beechem , Richard Haugland , Robert Archer , Rosaria Haugland
IPC分类号： G01N33/53
CPC分类号： B82Y5/00 , B82Y10/00 , G01N33/53
摘要： The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab′ fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.
摘要翻译：本发明提供标记试剂和用于标记一抗的标记试剂和使用包含靶结合抗体和一种或多种标记试剂的免疫标记复合物检测样品中靶标的方法。标记试剂包括单价抗体片段或非抗体单体蛋白质，由此标记的蛋白质对靶标结合抗体的特定区域具有亲和力，并且共价连接到标记物上。通常，标记试剂是通过用抗体的Fc片段免疫山羊或兔而产生的抗Fc Fab或Fab'片段。本发明提供标记试剂和免疫标记的复合物的离散子集，其有助于同时检测样品中的多个靶标，其中通过i）标记物与标记试剂的比率来区分免疫标记的复合物，或ii）物理或iii）标记试剂与所述靶结合抗体的比例，或iv）所述靶结合抗体。这对于可以连接到标记试剂和随后的用于检测多个靶的比例的免疫标记的复合物的荧光团标记物特别有用。

2. 发明申请

US20080039630A1 CYANINE COMPOUNDS AND THEIR APPLICATION AS QUENCHING COMPOUNDS 审中-公开
标题翻译：氰胺化合物及其作为化学化合物的应用
公开(公告)号：US20080039630A1
公开(公告)日：2008-02-14
申请号：US11782244
申请日：2007-07-24
申请人： Rosaria Haugland , Ching-Ying Cheung , Stephen Yue
发明人： Rosaria Haugland , Ching-Ying Cheung , Stephen Yue
IPC分类号： C07D401/10 , C07D209/18 , C07D403/10 , C07D403/14 , C07D417/10
CPC分类号： C12Q1/6876 , C07D209/12 , C07D209/14 , C07D209/24 , C07D401/04 , C09B23/12
摘要： The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or substituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.
摘要翻译：本发明提供了具有以下通式的方法和非荧光碳花青猝灭剂：其中A部分是取代的吡啶鎓，未取代的吡啶鎓，取代的喹啉鎓，未取代的喹啉鎓，取代的苯并唑鎓，未取代的苯并咪唑鎓，取代的吲哚鎓或取代的吲哚啉鎓。本发明还提供了发光供体分子猝灭剂对和发光供体分子 - 猝灭剂 - 发光受体分子缀合物，其中猝灭剂是本发明的花青化合物。能量转移对用于检测样品中感兴趣的分析物。

3. 发明授权

US08535894B2 Antibody complexes and methods for immunolabeling 有权
标题翻译：抗体复合物和免疫标记方法
公开(公告)号：US08535894B2
公开(公告)日：2013-09-17
申请号：US13610009
申请日：2012-09-11
申请人： Robert Archer , Richard Haugland , Rosaria Haugland , Joseph Beechem , David Hagen
发明人： Robert Archer , Richard Haugland , Rosaria Haugland , Joseph Beechem , David Hagen
IPC分类号： G01N33/53
CPC分类号： B82Y5/00 , B82Y10/00 , G01N33/53
摘要： Provided are labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Discrete subsets of labeling reagent and immune-labeled complexes are provided that facilitate the simultaneous detection of multiple targets in a sample-complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.
摘要翻译：提供标记试剂和用于标记一抗的标记试剂和用于使用包含靶结合抗体和一种或多种标记试剂的免疫标记复合物检测样品中的靶标的方法。标记试剂包括单价抗体片段或非抗体单体蛋白质，由此标记的蛋白质对靶标结合抗体的特定区域具有亲和力，并且共价连接到标记物上。提供标记试剂和免疫标记复合物的离散子集，其有利于同时检测样品中多个靶标的复合物，其特征在于i）标记与标记试剂的比例，或ii）所述标记的物理性质，或iii ）标记试剂与所述靶结合抗体的比例，或iv）所述靶结合抗体。这对于可以连接到标记试剂和随后的用于检测多个靶的比例的免疫标记的复合物的荧光团标记物特别有用。

4. 发明申请

US20050191643A1 Cyanine compounds and their application as quenching compounds 有权
标题翻译：花青化合物及其作为淬灭化合物的应用
公开(公告)号：US20050191643A1
公开(公告)日：2005-09-01
申请号：US10916822
申请日：2004-08-11
申请人： Rosaria Haugland , Ching-Ying Cheung , Stephen Yue
发明人： Rosaria Haugland , Ching-Ying Cheung , Stephen Yue
IPC分类号： C07D209/12 , C07D209/24 , C07D401/04 , C07D471/02 , C07D491/02 , C07D498/02 , C12Q1/68
CPC分类号： C12Q1/6876 , C07D209/12 , C07D209/14 , C07D209/24 , C07D401/04 , C09B23/12
摘要： The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or substituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.
摘要翻译：本发明提供了具有以下通式的方法和非荧光碳花青猝灭剂：其中A部分是取代的吡啶鎓，未取代的吡啶鎓，取代的喹啉鎓，未取代的喹啉鎓，取代的苯并唑鎓，未取代的苯并咪唑鎓，取代的吲哚鎓或取代的吲哚啉鎓。本发明还提供了发光供体分子猝灭剂对和发光供体分子 - 猝灭剂 - 发光受体分子缀合物，其中猝灭剂是本发明的花青化合物。能量转移对用于检测样品中感兴趣的分析物。

5. 发明申请

US20050069962A1 Antibody complexes and methods for immunolabeling 审中-公开
标题翻译：抗体复合物和免疫标记方法
公开(公告)号：US20050069962A1
公开(公告)日：2005-03-31
申请号：US10467550
申请日：2002-10-02
申请人： Robert Archer , Joseph Beechem , David Hagen , Richard Haugland , Rosaria Haugland
发明人： Robert Archer , Joseph Beechem , David Hagen , Richard Haugland , Rosaria Haugland
IPC分类号： G01N33/53
CPC分类号： B82Y5/00 , B82Y10/00 , G01N33/53
摘要： The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab′ fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.
摘要翻译：本发明提供标记试剂和用于标记一抗的标记试剂和使用包含靶结合抗体和一种或多种标记试剂的免疫标记复合物检测样品中靶标的方法。标记试剂包括单价抗体片段或非抗体单体蛋白质，由此标记的蛋白质对靶标结合抗体的特定区域具有亲和力，并且共价连接到标记物上。通常，标记试剂是通过用抗体的Fc片段免疫山羊或兔而产生的抗Fc Fab或Fab'片段。本发明提供标记试剂和免疫标记的复合物的离散子集，其有助于同时检测样品中的多个靶标，其中通过i）标记物与标记试剂的比率来区分免疫标记的复合物，或ii）物理或iii）标记试剂与所述靶结合抗体的比例，或iv）所述靶结合抗体。这对于可以连接到标记试剂和随后的用于检测多个靶的比例的免疫标记的复合物的荧光团标记物特别有用。

6. 发明授权

US07271265B2 Cyanine compounds and their application as quenching compounds 有权
标题翻译：花青化合物及其作为淬灭化合物的应用
公开(公告)号：US07271265B2
公开(公告)日：2007-09-18
申请号：US10916822
申请日：2004-08-11
申请人： Rosaria Haugland , Ching-Ying Cheung , Stephen Yue
发明人： Rosaria Haugland , Ching-Ying Cheung , Stephen Yue
IPC分类号： C07D401/10 , C07D417/10 , C07D403/10
CPC分类号： C12Q1/6876 , C07D209/12 , C07D209/14 , C07D209/24 , C07D401/04 , C09B23/12
摘要： The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or unsubstituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.
摘要翻译：本发明提供了具有以下通式的方法和非荧光碳花青猝灭剂化合物：其中A部分是取代的吡啶鎓，未取代的吡啶鎓，取代的喹啉鎓，未取代的喹啉鎓，取代的苯并唑鎓，未取代的苯并咪唑鎓，取代的二氢吲哚鎓或未取代的吲哚啉鎓。本发明还提供了发光供体分子猝灭剂对和发光供体分子 - 猝灭剂 - 发光受体分子缀合物，其中猝灭剂是本发明的花青化合物。能量转移对用于检测样品中感兴趣的分析物。

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