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1. 发明授权

US06777589B1 Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效
标题翻译：用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物
公开(公告)号：US06777589B1
公开(公告)日：2004-08-17
申请号：US08440646
申请日：1995-05-15
申请人： Ronald C. Lundquist , David A. Walters
发明人： Ronald C. Lundquist , David A. Walters
IPC分类号： C12N1500
CPC分类号： C12N15/8254 , C07K14/325 , C07K14/425 , C12N15/8207 , C12N15/8251 , C12N15/8253 , C12N15/8286 , Y02A40/162
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stablefs transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译：本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

2. 发明授权

US06331665B1 Insect resistant fertile transgenic corn plants 失效
标题翻译：抗虫育性转基因玉米植物
公开(公告)号：US06331665B1
公开(公告)日：2001-12-18
申请号：US08619077
申请日：1996-03-20
申请人： Ronald C. Lundquist , David A. Walters
发明人： Ronald C. Lundquist , David A. Walters
IPC分类号： A01H500
CPC分类号： C12N15/8274 , A01H5/10 , C07K14/325 , C07K14/425 , C12N9/1092 , C12N15/8207 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12N15/8275 , C12N15/8286 , Y02A40/162
摘要： Fertile transgenic Zea mays (corn) plants which stably express recombinant DNA which is heritable are provided wherein said DNA preferably comprises a recombinant gene which encodes a seed storage protein, so that the amino acid profile of the corn is improved.
摘要翻译：提供稳定表达可遗传的重组DNA的可育转基因玉米（玉米）植物，其中所述DNA优选包含编码种子储存蛋白的重组基因，从而提高玉米的氨基酸分布。

3. 发明授权

US06329574B1 High lysine fertile transgenic corn plants 失效
标题翻译：高赖氨酸可育转基因玉米植株
公开(公告)号：US06329574B1
公开(公告)日：2001-12-11
申请号：US09122399
申请日：1998-07-24
申请人： Ronald C. Lundquist , David A. Walters , Julie A. Kirihara
发明人： Ronald C. Lundquist , David A. Walters , Julie A. Kirihara
IPC分类号： C12N1500
CPC分类号： C07K14/325 , C07K14/425 , C12N15/8207 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12N15/8275 , C12N15/8286 , Y02A40/162
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stablefs transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译：本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

4. 发明授权

US6025545A Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效
标题翻译：用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物
公开(公告)号：US6025545A
公开(公告)日：2000-02-15
申请号：US440689
申请日：1995-05-15
申请人： Ronald C. Lundquist , David A. Walters , T. Michael Spencer , Catherine J. Mackey
发明人： Ronald C. Lundquist , David A. Walters , T. Michael Spencer , Catherine J. Mackey
IPC分类号： C07K14/325 , C07K14/425 , C12N15/82 , A01H1/06 , A01H4/00 , C12N15/00
CPC分类号： C07K14/325 , C07K14/425 , C12N15/8207 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12N15/8286
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stables transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译：本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。本文公开的实现马厩转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

5. 发明授权

US6013863A Fertile transgenic corn plants 失效
标题翻译：可育转基因玉米植物
公开(公告)号：US6013863A
公开(公告)日：2000-01-11
申请号：US844555
申请日：1997-04-21
申请人： Ronald C. Lundquist , David A. Walters
发明人： Ronald C. Lundquist , David A. Walters
IPC分类号： C07K14/325 , C07K14/425 , C12N15/82 , C12N15/00 , A01H1/06 , A01H4/00
CPC分类号： C07K14/325 , C07K14/425 , C12N15/8207 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12N15/8274 , C12N15/8286
摘要： A process of preparing fertile Zea mays plants is provided wherein said plants are resistant to the herbicide glyphosate, as well as processes for preparing seed, human food or animal feed therefrom.
摘要翻译：提供了一种制备可育玉米植物的方法，其中所述植物对除草剂草甘膦具有抗性，以及从其制备种子，人类食物或动物饲料的方法。

6. 发明授权

US5780708A Fertile transgenic corn plants 失效
标题翻译：达拉邦抗性可育转基因玉米植物
公开(公告)号：US5780708A
公开(公告)日：1998-07-14
申请号：US618749
申请日：1996-03-20
申请人： Ronald C. Lundquist , David A. Walters
发明人： Ronald C. Lundquist , David A. Walters
IPC分类号： A01H5/00 , C07K14/325 , C07K14/425 , C12N5/10 , C12N15/09 , C12N15/82 , A01H4/00 , C12N15/06
CPC分类号： C12N15/8274 , A01H5/10 , C07K14/325 , C07K14/425 , C12N15/8207 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12N15/8275 , C12N15/8286 , C12N9/1092
摘要： Fertile transgenic Zea mays (corn) plants which stably express recombinant DNA which is heritable are provided wherein said DNA preferably comprises a recombinant gene which encodes a seed storage protein, so that the amino acid profile of the corn is improved.
摘要翻译：提供稳定表达可遗传的重组DNA的可育转基因玉米（玉米）植物，其中所述DNA优选包含编码种子储存蛋白的重组基因，从而提高玉米的氨基酸分布。

7. 发明授权

US5538880A Method for preparing fertile transgenic corn plants 失效
标题翻译：制备可育转基因玉米植物的方法
公开(公告)号：US5538880A
公开(公告)日：1996-07-23
申请号：US249458
申请日：1994-05-26
申请人： Ronald C. Lundquist , David A. Walters
发明人： Ronald C. Lundquist , David A. Walters
IPC分类号： C07K14/325 , C07K14/425 , C12N15/82 , C12N15/87 , C12N15/00 , C12N15/05 , A01H1/06 , A01H4/00
CPC分类号： C07K14/325 , C07K14/425 , C12N15/8207 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12N15/8286
摘要： Fertile transgenic Zea mays (corn) plants which stably express heterologous DNA which is heritable are disclosed along with a process for producing said plants. The process comprises the microjectile bombardment of friable embryogenic callus from the plant to be transformed. The process may be applicable to other graminaceous cereal plants which have not proven stably transformable by other techniques.
摘要翻译：与生产所述植物的方法一起公开了稳定表达可遗传的异源DNA的可育转基因玉米（玉米）植物。该过程包括从待转化的植物中的脆性胚发生愈伤组织的微孔轰击。该方法可适用于尚未被其他技术稳定转化的其他禾本科谷物植物。

8. 发明授权

US5538877A Method for preparing fertile transgenic corn plants 失效
公开(公告)号：US5538877A
公开(公告)日：1996-07-23
申请号：US974379
申请日：1992-11-10
申请人： Ronald C. Lundquist , David A. Walters
发明人： Ronald C. Lundquist , David A. Walters
IPC分类号： C07K14/325 , C07K14/425 , C12N15/82 , C12N15/87 , C12N15/00 , C12N15/05 , A01H1/06 , A01H4/00
CPC分类号： C07K14/325 , C07K14/425 , C12N15/8207 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12N15/8286
摘要： Fertile transgenic Zea mays (corn) plants which stably express heterologous DNA which is heritable are disclosed along with a process for producing said plants. The process comprises the microjectile bombardment of friable embryogenic callus from the plant to be transformed. The process may be applicable to other graminaceous cereal plants which have not proven stably transformable by other techniques.

9. 发明授权

US5508468A Fertile transgenic corn plants 失效
公开(公告)号：US5508468A
公开(公告)日：1996-04-16
申请号：US285488
申请日：1994-08-03
申请人： Ronald C. Lundquist , David A. Walters , Julie A. Kirihara
发明人： Ronald C. Lundquist , David A. Walters , Julie A. Kirihara
IPC分类号： A01H5/00 , C07K14/325 , C07K14/425 , C12N5/10 , C12N15/09 , C12N15/82 , C12N15/00 , A01H1/06
CPC分类号： C12N15/8274 , A01H5/10 , C07K14/325 , C07K14/425 , C12N15/8207 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12N15/8275 , C12N15/8286 , C12N9/1092
摘要： Fertile transgenic Zea mays (corn) plants which stably express recombinant DNA which is heritable are provided wherein said DNA preferably comprises a recombinant gene which encodes a seed storage protein, so that the amino acid profile of the corn is improved.

10. 发明授权

US4605627A Plasmid vehicle for cloning in Agrobacterium tumefaciens 失效
标题翻译：用于在根癌土壤杆菌中克隆的质粒载体
公开(公告)号：US4605627A
公开(公告)日：1986-08-12
申请号：US501959
申请日：1983-06-07
申请人： Clarence Kado , Robert C. Tait , Ronald C. Lundquist
发明人： Clarence Kado , Robert C. Tait , Ronald C. Lundquist
IPC分类号： C12N15/82 , C12N1/00 , C07H15/12 , C12N15/00
CPC分类号： C12N15/8205
摘要： A cloning vector comprises a replication system derived from the pTAR plasmid and capable of stable maintenance in Agrobacterium tumefaciens. By combining the pTAR replication system with a second replication system from a host other than A. tumefaciens, shuttle vectors are obtained which allow manipulation in more than one host. The cloning vectors will usually include selectable markers having restriction enzyme sites which allow identification of recombinant molecules by insertional inactivation. By providing at least a fragment of the T-DNA region from the Ti plasmid, the subject vectors can be used to clone desired DNA fragments and transfer these fragments to the genome of a higher plant.The strain E. coli HB101/puc0400 was deposited on June 7, 1983 at the A.T.C.C. for patent purposes and granted accession no. 39377.
摘要翻译：克隆载体包含衍生自pTAR质粒并能够在根癌土壤杆菌中稳定维持的复制系统。通过将pTAR复制系统与来自根瘤土壤杆菌以外的宿主的第二复制系统相结合，获得允许在多于一个宿主中操纵的穿梭载体。克隆载体通常包括具有限制酶位点的选择标记，其允许通过插入失活来鉴定重组分子。通过从Ti质粒提供至少一个T-DNA区的片段，可以使用目标载体来克隆所需的DNA片段并将这些片段转移到较高植物的基因组。大肠杆菌HB101 / puc0400菌株于1983年6月7日在A.T.C.C. 专利用途并授予加入号。 39377。

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