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1. 发明申请

US20050112647A1 Detection system 审中-公开
标题翻译：检测系统
公开(公告)号：US20050112647A1
公开(公告)日：2005-05-26
申请号：US10958377
申请日：2004-10-06
申请人： Martin Lee , Roderick Fuerst
发明人： Martin Lee , Roderick Fuerst
IPC分类号： G01N33/50 , C12N15/09 , C12Q1/68 , C12Q1/6818 , C12Q1/6823
CPC分类号： C12Q1/6818 , C12Q1/6823 , Y10S436/80 , Y10T436/143333 , C12Q2563/107 , C12Q2561/113 , C12Q2527/107 , C12Q2563/173
摘要： A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising: (a) adding to a sample suspected of containing said target nucleic acid sequence, a probe specific for said target sequence and DNA duplex binding agent, said probe comprising a reactive molecule able to absorb fluorescence from or donate fluorescent energy to said DNA duplex binding agent, (b) subjecting the thus formed mixture to an amplification reaction in which target nucleic acid is amplified, (c) subjecting said sample to conditions under which the said probe hybridizes to the target sequence, and (d) monitoring fluorescence from said sample. This method can be used for example to monitor amplification reactions such as PCR reactions, such that the amount of target sequence present in the sample may be determined. Additionally or alternatively, it may be used to generate duplex destabilization data such as melt hysteresis information for amplification monitoring or for detection and quantitation of polymorphisms or allelic variation, and so is useful in genetic diagnosis.
摘要翻译：一种用于检测样品中靶核酸序列的存在的方法，所述方法包括：（a）向疑似含有所述靶核酸序列的样品，特异于所述靶序列的探针和DNA双链体结合剂加入所述探针，其包含能够从所述DNA双链体结合剂吸收荧光能量或向其提供荧光能量的反应性分子，（b）使由此形成的混合物经受扩增反应，其中靶核酸被扩增，（c）使所述样品经受所述探针与目标序列杂交，和（d）监测来自所述样品的荧光。该方法可用于例如监测扩增反应如PCR反应，使得可以确定样品中存在的靶序列的量。另外或替代地，其可以用于产生双相不稳定数据，例如用于放大监测的熔体滞后信息或用于多态性或等位基因变异的检测和定量，因此在遗传诊断中是有用的。

2. 发明授权

US06833257B2 Fluorimetric detection system of a nucleic acid 有权
标题翻译：核酸的荧光检测系统
公开(公告)号：US06833257B2
公开(公告)日：2004-12-21
申请号：US09555123
申请日：2000-05-25
申请人： Martin A Lee , Roderick Fuerst
发明人： Martin A Lee , Roderick Fuerst
IPC分类号： C12D1934
CPC分类号： C12Q1/6818 , C12Q1/6823 , Y10S436/80 , Y10T436/143333 , C12Q2563/107 , C12Q2561/113 , C12Q2527/107 , C12Q2563/173
摘要： A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising: (a) adding to a sample suspected of containing said target nucleic acid sequence, a probe specific for said target sequence and DNA duplex binding agent, said probe comprising a reactive molecule able to absorb fluorescence from or donate fluorescent energy to said DNA duplex binding agent, (b) subjecting the thus formed mixture to an amplification reaction in which target nucleic acid is amplified, (c) subjecting said sample to conditions under which the said probe hybridizes to the target sequence, and (d) monitoring fluorescence from said sample. This method can be used for example to monitor amplification reactions such as PCR reactions, such that the amount of target sequence present in the sample may be determined. Additionally or alternatively, it may be used to generate duplex destabilization data such as melt hysteresis information for amplification monitoring or for detection and quantitation of polymorphisms or allelic variation, and so is useful in genetic diagnosis.
摘要翻译：一种用于检测样品中靶核酸序列的存在的方法，所述方法包括：（a）向疑似含有所述靶核酸序列的样品，特异于所述靶序列的探针和DNA双链体结合剂加入所述探针，其包含能够从所述DNA双链体结合剂吸收荧光能量或向其提供荧光能量的反应性分子，（b）使由此形成的混合物经受扩增反应，其中靶核酸被扩增，（c）使所述样品经受所述探针与靶序列杂交，以及（d）监测来自所述样品的荧光。该方法可用于例如监测扩增反应如PCR反应，使得可以确定样品中存在的靶序列的量。另外或替代地，其可以用于产生双相不稳定数据，例如用于放大监测的熔体滞后信息或用于多态性或等位基因变异的检测和定量，因此在遗传诊断中是有用的。

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