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1. 发明授权

US06428703B1 Method for purifying biological marcromolecules, and chromatographic column for performing said method 有权
标题翻译：纯化生物大分子的方法，以及用于进行所述方法的色谱柱
公开(公告)号：US06428703B1
公开(公告)日：2002-08-06
申请号：US09471152
申请日：1999-12-23
申请人： Thomas Zinn , Manfred Sieber , Robert Zeidler
发明人： Thomas Zinn , Manfred Sieber , Robert Zeidler
IPC分类号： B01D1508
CPC分类号： B01D15/08 , B01D15/12 , B01D15/363 , G01N30/34
摘要： The invention concerns a method for purifying biological macromolecules, e.g. nucleic acids or proteins, from starting materials, in which the macromolecules, contained in a biological solution, are purified on a chromatographic material, and in which endotoxins are partially or completely removed with the aid of a nonionic surfactant, which is characterized in that the surfactant and the biological solution are brought into contact immediately before or during the purification of the macromolecules on the chromatographic material.
摘要翻译：本发明涉及一种纯化生物大分子的方法，来自原料的核酸或蛋白质，其中包含在生物溶液中的大分子在色谱材料上纯化，并且其中内毒素在非离子表面活性剂的帮助下部分或完全除去，其特征在于，表面活性剂和生物溶液在色谱材料上的大分子纯化之前或期间接触。

2. 发明授权

US06177009B1 Apparatus for treating biomolecules 有权
标题翻译：用于治疗生物分子的装置
公开(公告)号：US06177009B1
公开(公告)日：2001-01-23
申请号：US09262012
申请日：1999-03-04
申请人： Manfred Sieber , Robert Zeidler
发明人： Manfred Sieber , Robert Zeidler
IPC分类号： B01D1508
CPC分类号： G01N30/6065 , G01N2030/381 , G01N2030/527 , Y10T436/255
摘要： Disclosed is an apparatus for isolating nucleic acids having a separation column which has a top inlet and a bottom outlet in which a separation device is arranged, and having a collection vessel for collecting liquid emerging from the outlet. The ratio between the internal volume of the separation column above the separation device and the surface area of the separation device is at least 10 ml/cm2.
摘要翻译：公开了一种用于分离具有分离塔的核酸的装置，该分离塔具有顶部入口和底部出口，其中布置有分离装置，并且具有用于收集从出口出来的液体的收集容器。分离装置之上的分离塔的内部容积与分离装置的表面积之间的比例至少为10ml / cm2。

3. 发明申请

US20080213753A1 METHOD FOR THE VERIFICATION OF THE REMOVAL OF VIRUSES TO VALIDATE FILTERS AND FILTERING PROCESSES 审中-公开
标题翻译：用于验证去除病毒以验证过滤器和过滤过程的方法
公开(公告)号：US20080213753A1
公开(公告)日：2008-09-04
申请号：US12047470
申请日：2008-03-13
申请人： Hartmut HENNING , Oscar-Werner Reif , Klaus Tarrach , Robert Zeidler
发明人： Hartmut HENNING , Oscar-Werner Reif , Klaus Tarrach , Robert Zeidler
IPC分类号： C12Q1/70
CPC分类号： C12N7/00 , C12N2750/14051
摘要： Disclosed is a method for verification of the removal of viruses to validate filters, filtering processes, physical and chemical inactivation processes, or adsorptive removal processes in predefined process conditions that are simulated on a small scale. According to said method, viruses are cultured in suitable cell lines in a first step, a virus suspension is obtained after solubilizing cells in a second step, the virus suspension obtained is added in a third step to a protein solution that is to be analyzed, and the virus-containing protein solution is filtered through the filter that is to be validated, and the removal of viruses is then analyzed in a fourth step. The virus suspension is first processed via a membrane adsorber following step two, the viruses being bonded to the membrane adsorber and contaminants being removed with the aid of a detergent buffer solution, while the bonded, purified viruses are eluted from the membrane adsorber area and are added to the protein solution that is to be analyzed as a concentrated virus suspension in step three.
摘要翻译：公开了一种验证病毒去除以验证过滤器，过滤过程，物理和化学灭活过程或在小规模模拟的预定义工艺条件下的吸附去除过程的方法。根据所述方法，在第一步骤中，将病毒在合适的细胞系中培养，在第二步溶解细胞后获得病毒悬浮液，将获得的病毒悬浮液添加到待分析的蛋白质溶液中，并通过要验证的过滤器过滤病毒蛋白质溶液，然后在第四步骤中分析病毒的去除。首先在步骤2之后通过膜吸附器处理病毒悬浮液，病毒结合到膜吸附器上，借助于洗涤剂缓冲溶液除去污染物，而结合的纯化病毒从膜吸附区域洗脱出来，并且是添加到在步骤3中待浓缩的病毒悬浮液分析的蛋白质溶液中。

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