专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20130079497A1 PYRENYLOXYSULFONIC ACID FLUORESCENT AGENTS 有权
标题翻译：吡咯烷酮酸荧光剂
公开(公告)号：US20130079497A1
公开(公告)日：2013-03-28
申请号：US13592532
申请日：2012-08-23
申请人： Richard Haugland , Wai-Yee Leung , Jixiang Liu
发明人： Richard Haugland , Wai-Yee Leung , Jixiang Liu
IPC分类号： C07D211/60 , C07K16/18 , C07K14/77
CPC分类号： G01N33/536 , C07C309/38 , C07D207/16 , C07D207/46 , C07D211/56 , C07D211/60 , C07D211/62 , C07D401/12 , C07H17/08 , C07H19/04 , C07K14/77 , C07K16/18 , C09B57/001
摘要： The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.
摘要翻译：本发明提供了一种基于芘磺酸核的新型反应性荧光剂。通过使反应性基团与缀合物的其他物质上的互补反应性基团反应，将药剂容易地与其它物质结合。还提供了使用本发明化合物检测和/或定量样品中的分析物的方法。在一个示例性实施方案中，本发明提供掺入本发明化合物的多色测定法。

2. 发明授权

US08268886B2 Pyrenyloxysulfonic acid fluorescent agents 有权
标题翻译：吡喃氧基磺酸荧光剂
公开(公告)号：US08268886B2
公开(公告)日：2012-09-18
申请号：US13149444
申请日：2011-05-31
申请人： Richard Haugland , Wai-Yee Leung , Jixiang Liu
发明人： Richard Haugland , Wai-Yee Leung , Jixiang Liu
IPC分类号： A61K31/185 , A61K31/11 , G01N33/566 , C07C309/03 , C12P21/08
CPC分类号： G01N33/536 , C07C309/38 , C07D207/16 , C07D207/46 , C07D211/56 , C07D211/60 , C07D211/62 , C07D401/12 , C07H17/08 , C07H19/04 , C07K14/77 , C07K16/18 , C09B57/001
摘要： The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.
摘要翻译：本发明提供了一种基于芘磺酸核的新型反应性荧光剂。通过使反应性基团与缀合物的其他物质上的互补反应性基团反应，将药剂容易地与其它物质结合。还提供了使用本发明化合物检测和/或定量样品中的分析物的方法。在一个示例性实施方案中，本发明提供掺入本发明化合物的多色测定法。

3. 发明申请

US20120045848A1 PYRENYLOXYSULFONIC ACID FLUORESCENT AGENTS 有权
标题翻译：吡咯烷酮酸荧光剂
公开(公告)号：US20120045848A1
公开(公告)日：2012-02-23
申请号：US13149444
申请日：2011-05-31
申请人： Richard Haugland , Wai-Yee Leung , Jixiang Liu
发明人： Richard Haugland , Wai-Yee Leung , Jixiang Liu
IPC分类号： G01N21/64 , C07C309/61 , C07K16/18 , C07D207/46 , C07D207/404 , C07D211/62 , C07D207/16
CPC分类号： G01N33/536 , C07C309/38 , C07D207/16 , C07D207/46 , C07D211/56 , C07D211/60 , C07D211/62 , C07D401/12 , C07H17/08 , C07H19/04 , C07K14/77 , C07K16/18 , C09B57/001
摘要： The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.
摘要翻译：本发明提供了一种基于芘磺酸核的新型反应性荧光剂。通过使反应性基团与缀合物的其他物质上的互补反应性基团反应，将药剂容易地与其它物质结合。还提供了使用本发明化合物检测和/或定量样品中的分析物的方法。在一个示例性实施方案中，本发明提供掺入本发明化合物的多色测定法。

4. 发明授权

US08039642B2 Pyrenyloxysulfonic acid fluorescent agents 有权
标题翻译：吡喃氧基磺酸荧光剂
公开(公告)号：US08039642B2
公开(公告)日：2011-10-18
申请号：US11871596
申请日：2007-10-12
申请人： Jixiang Liu , Richard Haugland , Wai-Yee Leung
发明人： Jixiang Liu , Richard Haugland , Wai-Yee Leung
IPC分类号： C07D207/06 , C07D255/02
CPC分类号： G01N33/536 , C07C309/38 , C07D207/16 , C07D207/46 , C07D211/56 , C07D211/60 , C07D211/62 , C07D401/12 , C07H17/08 , C07H19/04 , C07K14/77 , C07K16/18 , C09B57/001
摘要： The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.
摘要翻译：本发明提供了一种基于芘磺酸核的新型反应性荧光剂。通过使反应性基团与缀合物的其他物质上的互补反应性基团反应，将药剂容易地与其它物质结合。还提供了使用本发明化合物检测和/或定量样品中的分析物的方法。在一个示例性实施方案中，本发明提供掺入本发明化合物的多色测定法。

5. 发明授权

US07871773B2 Aza-benzazolium containing cyanine dyes 有权
标题翻译：含有氰基染料的氮杂 -
公开(公告)号：US07871773B2
公开(公告)日：2011-01-18
申请号：US11756765
申请日：2007-06-01
申请人： Richard Haugland , Stephen Yue
发明人： Richard Haugland , Stephen Yue
IPC分类号： C12Q1/68 , C12Q1/00 , C12Q1/02 , G01N33/53 , C07D215/00 , C07D417/00 , C12P19/34
CPC分类号： C09B23/02 , C12Q1/6816 , C12Q1/6876 , G01N1/30 , G01N27/44726 , G01N33/52 , G01N33/5308 , G01N33/531 , G01N33/582 , G01N33/68 , G01N2001/302 , Y10S436/80 , Y10T436/143333 , C12Q2563/173 , C12Q2563/107
摘要： Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.
摘要翻译：描述了包含氮杂 - 苯并唑鎓环部分的不对称花青染料，包括由阳离子侧链取代的花青染料，单体和二聚花青染料，化学反应性花青染料和花青染料的缀合物。当在水溶液中稀释时，受试染料实际上是非荧光的，但当与核酸聚合物如DNA或RNA相关联时或当与洗涤剂复合的蛋白质相关时，它们显示出明亮的荧光。描述了用于检测和定量多种样品中的核酸和洗涤剂复合蛋白质的各种应用，包括溶液，电泳凝胶，细胞和微生物。

6. 发明申请

US20080044811A1 Aza-Benzazolium Containing Cyanine Dyes 有权
标题翻译：含有氰基染料的氮杂 - 唑烷鎓
公开(公告)号：US20080044811A1
公开(公告)日：2008-02-21
申请号：US11756765
申请日：2007-06-01
申请人： Richard Haugland , Stephen Yue
发明人： Richard Haugland , Stephen Yue
IPC分类号： C12Q1/70 , C12Q1/68 , G01N33/00 , G01N33/68
CPC分类号： C09B23/02 , C12Q1/6816 , C12Q1/6876 , G01N1/30 , G01N27/44726 , G01N33/52 , G01N33/5308 , G01N33/531 , G01N33/582 , G01N33/68 , G01N2001/302 , Y10S436/80 , Y10T436/143333 , C12Q2563/173 , C12Q2563/107
摘要： Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.
摘要翻译：描述了包含氮杂 - 苯并唑鎓环部分的不对称花青染料，包括由阳离子侧链取代的花青染料，单体和二聚花青染料，化学反应性花青染料和花青染料的缀合物。当在水溶液中稀释时，受试染料实际上是非荧光的，但当与核酸聚合物如DNA或RNA相关联时或当与洗涤剂复合的蛋白质相关时，它们显示出明亮的荧光。描述了用于检测和定量多种样品中的核酸和洗涤剂复合蛋白质的各种应用，包括溶液，电泳凝胶，细胞和微生物。

7. 发明授权

US07102005B2 Compositions and methods for detection and isolation of phosphorylated molecules 有权
标题翻译：用于磷酸化分子检测和分离的组合物和方法
公开(公告)号：US07102005B2
公开(公告)日：2006-09-05
申请号：US10428192
申请日：2003-05-02
申请人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Jixiang Liu , Vladimir Martin , Wayne Patton , Thomas Steinberg
发明人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Jixiang Liu , Vladimir Martin , Wayne Patton , Thomas Steinberg
IPC分类号： C07D239/88 , C07D277/62 , C07D311/82 , C07D417/02 , C07D495/04
CPC分类号： G01N33/50 , G01N33/5008 , G01N33/502 , G01N33/5091 , G01N2333/4709
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译：本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在磷酸化结合化合物和导致三元复合物的磷酸化靶分子。本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

8. 发明申请

US20050250957A1 Compounds containing thiosulfate moieties 审中-公开
标题翻译：含有硫代硫酸盐部分的化合物
公开(公告)号：US20050250957A1
公开(公告)日：2005-11-10
申请号：US10982301
申请日：2004-11-05
申请人： Richard Haugland , Hee Kang
发明人： Richard Haugland , Hee Kang
IPC分类号： C07C305/00 , C07C381/02 , C07D233/32 , C07D233/36 , C07D311/82 , C07D311/88 , C07D311/90 , C07D487/04 , C07F5/02 , C09B11/08 , C09B11/24 , C09B57/00 , C09B57/10 , G01N33/531 , G01N33/543
CPC分类号： C07D487/04 , C07C381/02 , C07D233/32 , C07D311/82 , C07D311/88 , C07D311/90 , C07F5/022 , C09B11/08 , C09B11/24 , C09B57/00 , C09B57/10 , G01N33/531 , G01N33/54353
摘要： The present invention provides chemical compounds containing a thiosulfate group, and methods for their preparation and use. The thiosulfate group reacts with a thiol group to form a disulfide bond.
摘要翻译：本发明提供含有硫代硫酸盐基团的化合物及其制备和使用方法。硫代硫酸盐基团与硫醇基团反应形成二硫键。

9. 发明申请

US20050250152A1 Reagents for labeling biomolecules having aldehyde or ketone moieties 有权
标题翻译：用于标记具有醛或酮部分的生物分子的试剂
公开(公告)号：US20050250152A1
公开(公告)日：2005-11-10
申请号：US11182122
申请日：2005-07-15
申请人： Richard Haugland , Thomas Steinberg , Wayne Patton , Zhenjun Diwu
发明人： Richard Haugland , Thomas Steinberg , Wayne Patton , Zhenjun Diwu
IPC分类号： G01N33/532 , C07D239/91 , C07D239/94 , C07D249/08 , C07D263/60 , C07D265/22 , C07D277/66 , C07D401/04 , G01N33/58 , C12Q1/68 , C07H21/04 , C08B37/00
CPC分类号： C07D265/22 , C07D239/91 , Y10T436/143333 , Y10T436/200833
摘要： Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. In one embodiment is provided reagents that have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5 is -L-Z; L is arylene, or a C1-6 perfluoroalkylene, or a single covalent bond; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14 are independently H, C1-6alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-21-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, or sulfo; and R21-R24 are independently H, C1-6 alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The method of treating a sample with the derivativization reagents is described. The reagents are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.
摘要翻译：描述了适用于与含有醛或酮官能团的生物分子偶联的新型荧光衍生试剂。在一个实施方案中，提供具有下式的试剂：其中Q是羰基，硫代羰基或磺酰基，R 5是-L-Z; L是亚芳基或C 1-6个全氟亚烷基，或单个共价键; Z是羰基酰肼，酰肼，磺酰肼或硫代羰基酰肼; R 11 -R 14独立地为H，C 1-6烷基，C 1-6烷氧基，C 全氟烷基，C 1-6烷基氨基，二（C 1 -C 21 - 亚烷基）氨基，氨基，羧基，氰基，卤素，羟基，硝基，苯基或磺基; R 21和R 24独立地是H，C 1-6烷基，C 1-6烷氧基， C 1-6烷基全氟烷基，C 1-6烷基氨基，二（C 2-12-12 - 烷基）氨基，氨基，羧基，氰基，卤素，羟基，硝基，苯基，磺基，或-LZ。描述了用衍生化试剂处理样品的方法。这些试剂特别可用于在电泳凝胶中标记糖蛋白或糖肽，核酸和脂多糖。

10. 发明申请

US20120270757A1 SITE-SPECIFIC LABELING OF AFFINITY TAGS IN FUSION PROTEINS 审中-公开
标题翻译：融合蛋白中的亲和标签的特定标签
公开(公告)号：US20120270757A1
公开(公告)日：2012-10-25
申请号：US13429972
申请日：2012-03-26
申请人： Kyle GEE , Courtenay Hart , Wai-Yee Leung , Wayne Patton , Aleksey Rukavishnikov , Richard Haugland , Zhenjun Diwu
发明人： Kyle GEE , Courtenay Hart , Wai-Yee Leung , Wayne Patton , Aleksey Rukavishnikov , Richard Haugland , Zhenjun Diwu
IPC分类号： G01N21/76 , C07F5/02 , C40B70/00 , C07D311/18
CPC分类号： G01N33/52 , A61K49/0021 , A61K49/0039 , A61K49/0041 , A61K49/0052 , C07D311/12 , C07D311/16 , C07D311/18 , C07F5/022 , G01N33/533 , G01N33/582
摘要： The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6.
摘要翻译：本发明提供了通过能够选择性地结合亲和标签来促进融合蛋白的检测和标记的方法和荧光化合物。荧光化合物具有通式A（B）n，其中A是荧光团，B是作为带电化学部分的结合结构域，其蛋白质或片段，n是1-6的整数，条件是蛋白质或其片段不是抗体或从抗体产生。本发明提供用于检测和标记含有多组氨酸亲和标签的融合蛋白的特异性荧光化合物和方法。这些化合物具有通式A（L）m（B）n，其中A是荧光团，L是连接体，B是乙酸结合域，m是1至4的整数，n是1至 6。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式