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1. 发明申请

US20070178498A1 Protein/Solubility Folding Assessed By Structural Complementation 有权
标题翻译：蛋白质/溶解度折叠由结构互补评估
公开(公告)号：US20070178498A1
公开(公告)日：2007-08-02
申请号：US11617191
申请日：2006-12-28
申请人： Philip Thomas , John Hunt , William Wigley , Rhesa Stidham
发明人： Philip Thomas , John Hunt , William Wigley , Rhesa Stidham
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： G01N33/502 , C07K1/113
摘要： Many proteins, when produced recombinantly, suffer from improper processing, folding and lack normal solubility. Modified proteins, including those indicative of disease states, also can have such defects. The present invention is directed to methods of identifying proper and improper protein folding, aberrant processing and/or insolubility. The method relies on the use of two components: a specialized fusion protein and structural complementation. The fusion protein contains sequences from the protein of interest and one portion of a marker protein that, by itself, is not active. A host cell then provides the remainder of the marker protein that serves to “complement” the function of the fused marker protein such that their association restores activity, permitting detection.
摘要翻译：当重组产生时，许多蛋白质经受不当的加工，折叠和缺乏正常的溶解度。修饰的蛋白质，包括指示疾病状态的蛋白质，也可能具有这样的缺陷。本发明涉及鉴定正确和不适当的蛋白质折叠，异常加工和/或不溶性的方法。该方法依赖于两个组成部分的使用：一种专门的融合蛋白和结构互补。融合蛋白包含来自目的蛋白质的序列和一部分本身不具有活性的标记蛋白质。宿主细胞然后提供标记蛋白的其余部分，其用于“补充”融合标记蛋白的功能，使得它们的缔合恢复活性，允许检测。

2. 发明申请

US20050019829A1 Protein/solubility folding assessed by structural complementation 有权
标题翻译：通过结构互补评估蛋白质/溶解度折叠
公开(公告)号：US20050019829A1
公开(公告)日：2005-01-27
申请号：US10748720
申请日：2003-12-30
申请人： Philip Thomas , John Hunt , William Wigley , Rhesa Stidham
发明人： Philip Thomas , John Hunt , William Wigley , Rhesa Stidham
IPC分类号： G01N33/50 , C07K1/113 , C12N15/09 , C12Q1/54 , G01N33/15 , G01N33/53 , C12N15/85
CPC分类号： G01N33/502 , C07K1/113
摘要： Many proteins, when produced recombinantly, suffer from improper processing, folding and lack normal solubility. Modified proteins, including those indicative of disease states, also can have such defects. The present invention is directed to methods of identifying proper and improper protein folding, aberrant processing and/or insolubility. The method relies on the use of two components: a specialized fusion protein and structural complementation. The fusion protein contains sequences from the protein of interest and one portion of a marker protein that, by itself, is not active. A host cell then provides the remainder of the marker protein that serves to “complement” the function of the fused marker protein such that their association restores activity, permitting detection.
摘要翻译：当重组产生时，许多蛋白质经受不当的加工，折叠和缺乏正常的溶解度。修饰的蛋白质，包括指示疾病状态的蛋白质，也可能具有这样的缺陷。本发明涉及鉴定正确和不适当的蛋白质折叠，异常加工和/或不溶性的方法。该方法依赖于两个组成部分的使用：一种专门的融合蛋白和结构互补。融合蛋白包含来自目的蛋白质的序列和一部分本身不具有活性的标记蛋白质。宿主细胞然后提供标记蛋白的其余部分，其用于“补充”融合标记蛋白的功能，使得它们的缔合恢复活性，允许检测。

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