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1. 发明申请

US20060211000A1 Methods, compositions, and kits for detection of microRNA 审中-公开
标题翻译：用于检测microRNA的方法，组合物和试剂盒
公开(公告)号：US20060211000A1
公开(公告)日：2006-09-21
申请号：US11084082
申请日：2005-03-21
申请人： Joseph Sorge , Rebecca Mullinax
发明人： Joseph Sorge , Rebecca Mullinax
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6809 , C12Q1/6816 , C12Q2533/107 , C12Q2545/114 , C12Q2525/207
摘要： The present invention provides methods, nucleic acids, compositions, and kits for detecting microRNA (miRNA) in samples. The methods comprise ligating two oligonucleotides together in an miRNA mediated fashion, and detection of the ligation product. The methods can further comprise amplification of the ligation product, such as by PCR. The nucleic acids, compositions, and kits typically comprise some or all of the components necessary to practice the method of the invention.
摘要翻译：本发明提供了用于在样品中检测微小RNA（miRNA）的方法，核酸，组合物和试剂盒。所述方法包括以miRNA介导的方式将两个寡核苷酸结合在一起，并检测连接产物。所述方法可以进一步包括连接产物的扩增，例如通过PCR扩增。核酸，组合物和试剂盒通常包括实施本发明方法所需的一些或全部成分。

2. 发明申请

US20060088822A1 Novel polymerase compositions and uses thereof 失效
标题翻译：新型聚合酶组合物及其用途
公开(公告)号：US20060088822A1
公开(公告)日：2006-04-27
申请号：US10319778
申请日：2002-12-13
申请人： Joseph Sorge , Rebecca Mullinax
发明人： Joseph Sorge , Rebecca Mullinax
IPC分类号： C12Q1/68 , C12P19/34 , C12N9/22
CPC分类号： C12Q1/686 , C12Q2521/319 , C12Q2521/101
摘要： The subject invention provides novel compositions containing a mixture of (a) an enzyme that possesses substantial 3′-5′ exonuclease activity (b) a DNA polymerase with less 3′-5′ exonuclease activity than the enzyme with substantial 3′-5′ exonuclease activity. Preferably, the DNA polymerase for inclusion in the compositions are DNA polymerases that substantially lack 3′-5′ exonuclease activity. A preferred embodiment of the invention is a composition comprising the Taq DNA polymerase (isolated from Thermus aquaticus) and the Pfu DNA polymerase (isolated from Pyrococcus furiosus). Another aspect of the invention is to provide methods for synthesizing polynucleotides, typically DNA, using compositions comprising an enzyme that possesses substantial 3′-5′ exonuclease activity and a DNA polymerase with less 3′-5′ exonuclease activity than the enzymes possessing substantial 3′-5′ exonuclease activity, preferably a DNA polymerase that substantially lacks 3′-5′ exonuclease activity. Another aspect of the invention involves the use the subject method of polynucleotide synthesis to carry out the synthesis step in a polymerase chain reaction experiment. Yet another aspect of the invention is to provide kits for the synthesis of polynucleotides, wherein the kits comprise an enzyme that possesses substantial 3′-5′ exonuclease activity and a DNA polymerase with less 3′-5′ exonuclease activity than the enzyme possessing substantial 3′-5′ exonuclease activity.
摘要翻译：本发明提供了包含（a）具有大量3'-5'核酸外切酶活性的酶的混合物的新型组合物（b）具有比实际3'-5'核酸外切酶活性大的3'-5'核酸外切酶活性的3' 核酸外切酶活性。优选地，包含在组合物中的DNA聚合酶是基本上缺乏3'-5'核酸外切酶活性的DNA聚合酶。本发明的优选实施方案是包含Taq DNA聚合酶（从Thermus aquaticus分离）和Pfu DNA聚合酶（从激烈热球菌分离的）的组合物。本发明的另一方面是提供使用包含具有大量3'-5'核酸外切酶活性的酶的组合物和具有比具有实质3的酶的3'-5'外切核酸酶活性少的DNA聚合酶合成多核苷酸（通常为DNA）的方法 '-5'核酸外切酶活性，优选基本上缺乏3'-5'核酸外切酶活性的DNA聚合酶。本发明的另一方面涉及使用聚合酶链反应实验中的多核苷酸合成的主题方法进行合成步骤。本发明的另一方面是提供用于合成多核苷酸的试剂盒，其中所述试剂盒包含具有显着3'-5'核酸外切酶活性的酶和具有比具有实质性的酶的酶更少3'-5'外切核酸酶活性的DNA聚合酶 3'-5'核酸外切酶活性。

3. 发明申请

US20050123940A1 Compositions and methods for synthesizing cDNA 审中-公开
标题翻译：合成cDNA的组合物和方法
公开(公告)号：US20050123940A1
公开(公告)日：2005-06-09
申请号：US10853973
申请日：2004-05-26
申请人： Joseph Sorge , Holly Hogrefe , Connie Hansen , Bahram Arezi , Rebecca Mullinax
发明人： Joseph Sorge , Holly Hogrefe , Connie Hansen , Bahram Arezi , Rebecca Mullinax
IPC分类号： C12N9/12 , C12N9/22 , C12N15/10 , C12P19/34 , C12Q1/68
CPC分类号： B82Y5/00 , B82Y10/00 , C12N9/1252 , C12N9/1276 , C12N15/1096
摘要： The present invention relates to composition, kits and methods comprising a mutant DNA polymerase exhibiting increased reverse transcriptase activity. The invention also relates to methods of generating modified cDNA.
摘要翻译：本发明涉及包含显示增加的逆转录酶活性的突变型DNA聚合酶的组合物，试剂盒和方法。本发明还涉及产生修饰的cDNA的方法。

4. 发明申请

US20070065874A1 Compositions and methods comprising control nucleic acid 审中-公开
标题翻译：包含对照核酸的组合物和方法
公开(公告)号：US20070065874A1
公开(公告)日：2007-03-22
申请号：US11599936
申请日：2006-11-14
申请人： Rebecca Mullinax , Alexey Novoradovsky , Joseph Sorge
发明人： Rebecca Mullinax , Alexey Novoradovsky , Joseph Sorge
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6837 , C12Q1/6848 , C12Q2545/101
摘要： The present invention relates, in part, to control nucleic acid molecules having no significant sequence homology to any known nucleic acid, and predefined G/C-content. The present invention further relates to method of using control nucleic acid molecules to validate microarray analyses, compositions comprising control nucleic acid molecules, and kits comprising control nucleic acid molecules.
摘要翻译：本发明部分涉及控制与任何已知核酸无显着序列同源性的核酸分子和预定义的G / C含量。本发明还涉及使用对照核酸分子来验证微阵列分析的方法，包含对照核酸分子的组合物和包含对照核酸分子的试剂盒。

5. 发明申请

US20060014205A1 Novel polymerase compositions and uses thereof 审中-公开
公开(公告)号：US20060014205A1
公开(公告)日：2006-01-19
申请号：US11230945
申请日：2005-09-19
申请人： Joseph Sorge , Rebecca Mullinax
发明人： Joseph Sorge , Rebecca Mullinax
IPC分类号： C12Q1/68 , C12N9/22
CPC分类号： C12N9/1252 , C12Q1/686
摘要： The subject invention provides novel compositions containing a mixture of (a) an enzyme that possesses substantial 3′-5′ exonuclease activity (b) a DNA polymerase with less 3′-5′ exonuclease activity than the enzyme with substantial 3′-5′ exonuclease activity. Preferably, the DNA polymerase for inclusion in the compositions are DNA polymerases that substantially lack 3′-5′ exonuclease activity. A preferred embodiment of the invention is a composition comprising the Taq DNA polymerase (isolated from Thermus aquaticus) and the Pfu DNA polymerase (isolated from Pyrococcus furiosus). Another aspect of the invention is to provide methods for synthesizing polynucleotides, typically DNA, using compositions comprising an enzyme that possesses substantial 3′-5′ exonuclease activity and a DNA polymerase with less 3′-5′ exonuclease activity than the enzymes possessing substantial 3′-5′ exonuclease activity, preferably a DNA polymerase that substantially lacks 3′-5′ exonuclease activity. Another aspect of the invention involves the use the subject method of polynucleotide synthesis to carry out the synthesis step in a polymerase chain reaction experiment. Yet another aspect of the invention is to provide kits for the synthesis of polynucleotides, wherein the kits comprise an enzyme that possesses substantial 3′-5′ exonuclease activity and a DNA polymerase with less 3′-5′ exonuclease activity than the enzyme possessing substantial 3′-5′ exonuclease activity.

6. 发明申请

US20060286562A1 Methods for purifying DNA polymerases 审中-公开
标题翻译： DNA聚合酶纯化方法
公开(公告)号：US20060286562A1
公开(公告)日：2006-12-21
申请号：US11283531
申请日：2005-11-18
申请人： Ronda Allen , Daniel McMullan , Rebecca Mullinax
发明人： Ronda Allen , Daniel McMullan , Rebecca Mullinax
IPC分类号： C12Q1/68 , C12N9/22
CPC分类号： C12N9/1252
摘要： The present invention provides methods and kits for obtaining substantially pure DNA polymerases. The methods comprise fractionating preparations comprising at east one DNA polymerase using Poly U Sepharose chromatography and obtaining substantially pure DNA polymerase. The present invention also provides compositions comprising substantially pure archaebacterial DNA polymerase obtained by fractionation using Poly U Sepharose chromatography resin.
摘要翻译：本发明提供用于获得基本上纯的DNA聚合酶的方法和试剂盒。所述方法包括使用Poly U Sepharose色谱法在东一DNA聚合酶上包含分馏制剂，并获得基本上纯的DNA聚合酶。本发明还提供了包含通过使用Poly U Sepharose色谱树脂分级而获得的基本上纯的古细菌DNA聚合酶的组合物。

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