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1. 发明授权

US08597909B2 Use of HSA-producing cells 失效
标题翻译：使用HSA生产细胞
公开(公告)号：US08597909B2
公开(公告)日：2013-12-03
申请号：US13062781
申请日：2009-09-09
申请人： Lore Florin , Rebecca Bischoff , Juergen Fieder , Hitto Kaufmann , Thomas Krieg
发明人： Lore Florin , Rebecca Bischoff , Juergen Fieder , Hitto Kaufmann , Thomas Krieg
IPC分类号： C12P21/06
CPC分类号： C12N5/0037 , C12N2500/30 , C12N2502/99 , C12N2510/02
摘要： The present invention refers to the area of cell culture technology and relates to methods for multiplying/cloning cells, preferably cell lines, which are important to the production of biopharmaceuticals. The invention further relates to methods for manufacturing proteins and to the use of cells extracted and multiplied through single cell sorting and to media compositions that enable a multiplication of single cells. Through the use of albumin-producing, preferably HSA-producing, cells as feeder cells for the conditioning of medium or as host cells, the recloning efficiency and thereby the quantity of clones obtained can be significantly increased. A combination of these approaches is also possible. Through the use of albumin-producing, preferably HSA-producing, cells, an increase in the recloning efficiency can be achieved in serum-free and/or insulin-free medium as well, and in different cell types.
摘要翻译：本发明涉及细胞培养技术的领域，涉及对生物药物生产重要的细胞，优选细胞系的繁殖/克隆方法。本发明还涉及用于制造蛋白质的方法以及使用通过单细胞分选提取和倍增的细胞以及能够使单细胞增殖的培养基组合物的方法。通过使用生产白蛋白，优选产生HSA的细胞作为培养基或宿主细胞调节的饲养细胞，可以显着提高获得的克隆效率，从而获得克隆的量。这些方法的组合也是可能的。通过使用产生白蛋白，优选产生HSA的细胞，可以在无血清和/或不含胰岛素的培养基以及不同细胞类型中实现再生能力的提高。

2. 发明申请

US20110262967A1 USE OF HSA-PRODUCING CELLS 失效
标题翻译：使用HSA生产细胞
公开(公告)号：US20110262967A1
公开(公告)日：2011-10-27
申请号：US13062781
申请日：2009-09-09
申请人： Lore Florin , Rebecca Bischoff , Juergen Fieder , Hitto Kaufmann , Thomas Krieg
发明人： Lore Florin , Rebecca Bischoff , Juergen Fieder , Hitto Kaufmann , Thomas Krieg
IPC分类号： C12P21/00 , C12N5/071
CPC分类号： C12N5/0037 , C12N2500/30 , C12N2502/99 , C12N2510/02
摘要： The present invention refers to the area of cell culture technology and relates to methods for multiplying/cloning cells, preferably cell lines, which are important to the production of biopharmaceuticals. The invention further relates to methods for manufacturing proteins and to the use of cells extracted and multiplied through single cell sorting and to media compositions that enable a multiplication of single cells. Through the use of albumin-producing, preferably HSA-producing, cells as feeder cells for the conditioning of medium or as host cells, the recloning efficiency and thereby the quantity of clones obtained can be significantly increased. A combination of these approaches is also possible. Through the use of albumin-producing, preferably HSA-producing, cells, an increase in the recloning efficiency can be achieved in serum-free and/or insulin-free medium as well, and in different cell types.
摘要翻译：本发明涉及细胞培养技术的领域，涉及对生物药物生产重要的细胞，优选细胞系的繁殖/克隆方法。本发明还涉及用于制造蛋白质的方法以及使用通过单细胞分选提取和倍增的细胞以及能够使单细胞增殖的培养基组合物的方法。通过使用生产白蛋白，优选产生HSA的细胞作为培养基或宿主细胞调节的饲养细胞，可以显着提高获得的克隆效率，从而获得克隆的量。这些方法的组合也是可能的。通过使用产生白蛋白，优选产生HSA的细胞，可以在无血清和/或不含胰岛素的培养基以及不同细胞类型中实现再生能力的提高。

3. 发明申请

US20110281301A1 THE SECRETORY CAPACITY IN HOST CELLS 审中-公开
标题翻译：宿主细胞中的秘密能力
公开(公告)号：US20110281301A1
公开(公告)日：2011-11-17
申请号：US12742299
申请日：2008-10-06
申请人： Hitto Kaufmann , Eric Becker , Lore Florin , Barbara Enenkel , Kerstin Sautter , Rebecca Bischoff
发明人： Hitto Kaufmann , Eric Becker , Lore Florin , Barbara Enenkel , Kerstin Sautter , Rebecca Bischoff
IPC分类号： C12P21/00 , C12N5/10 , C12N15/85
CPC分类号： C12N5/0018 , C07K16/00 , C12N2501/48 , C12N2501/60 , C12N2510/02
摘要： The invention concerns the field of protein production and cell culture technology. It describes a method of producing a heterologous protein of interest in a cell comprising a. Increasing the expression or activity of a secretion enhancing gene, and b. Increasing the expression or activity of an anti-apoptotic gene, and c. Effecting the expression of said protein of interest, whereby the secretion enhancing gene is a gene encoding a protein whose expression or activity is induced during one of the following cellular processes: plasma-cell differentiation, unfolded protein response (UPR), endoplasmic reticulum overload response (EOR).
摘要翻译：本发明涉及蛋白质生产和细胞培养技术领域。它描述了在包含a的细胞中产生感兴趣的异源蛋白质的方法。增加分泌增强基因的表达或活性，b。增加抗凋亡基因的表达或活性，c。影响所述目的蛋白质的表达，由此分泌增强基因是编码在以下细胞过程之一期间诱导其表达或活性的蛋白质的基因：血浆细胞分化，展开的蛋白质应答（UPR），内质网过载反应（EOR）。

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