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1. 发明授权

US07604935B2 Soluble rubella E1 envelope protein variants 有权
标题翻译：可溶性风疹E1包膜蛋白变体
公开(公告)号：US07604935B2
公开(公告)日：2009-10-20
申请号：US11584887
申请日：2006-10-23
申请人： Barbara Upmeier , Ralf Bollhagen , Alfred Engel , Elke Faatz , Peter Schaarschmidt , Christian Scholz , Toralf Zarnt
发明人： Barbara Upmeier , Ralf Bollhagen , Alfred Engel , Elke Faatz , Peter Schaarschmidt , Christian Scholz , Toralf Zarnt
IPC分类号： C12Q1/70 , C12N7/01 , A61K39/20
CPC分类号： C07K14/005 , C07K2319/35 , C12N2770/36222 , Y10S530/826
摘要： A soluble rubella E1 antigen variant is disclosed that comprises amino acids 334-409 of the native rubella E1 peptide, but lacks the C-terminal end and at least the transmembrane region and the anchor segment as well as at least the amino acids 143 to 164. Also described is a recombinant DNA molecule encoding the rubella E1 antigen variants which are recombinantly expressed as a chaperone fusion protein, refolded into a soluble and immunoreactive conformation, and further used for the serological detection of anti-rubella antibodies. In addition, also disclosed is a method for the detection, determination and quantification of anti-rubella antibodies of IgG and/or IgM subclass in a sample wherein the rubella E1 antigen is used as a capture reagent and/or binding partner for the antibodies.
摘要翻译：公开了可溶性风疹E1抗原变体，其包含天然风疹E1肽的氨基酸334-409，但缺少C末端和至少跨膜区和锚定区以及至少氨基酸143至164 还描述了编码风疹E1抗原变体的重组DNA分子，其被重组表达为伴侣融合蛋白，重折叠成可溶性和免疫反应性构象，并进一步用于抗风疹抗体的血清学检测。此外，还公开了用于检测，测定和定量样品中IgG和/或IgM亚类的抗风疹抗体的方法，其中所述风疹E1抗原用作抗体的捕获试剂和/或结合配偶体。

2. 发明授权

US08551696B2 Rubella E1 envelope protein variants and their use in detection of anti-rubella antibodies 有权
标题翻译：风疹E1包膜蛋白变体及其在抗风疹抗体检测中的应用
公开(公告)号：US08551696B2
公开(公告)日：2013-10-08
申请号：US12793134
申请日：2010-06-03
申请人： Christian Scholz , Ralf Bollhagen , Alfred Engel , Elke Faatz , Peter Schaarschmidt , Barbara Upmeier , Toralf Zarnt
发明人： Christian Scholz , Ralf Bollhagen , Alfred Engel , Elke Faatz , Peter Schaarschmidt , Barbara Upmeier , Toralf Zarnt
IPC分类号： C12Q1/70 , A61K39/20 , C12N7/01
CPC分类号： G01N33/56983 , C07K14/005 , C07K2319/35 , C12N2770/36222 , G01N2333/19 , G01N2469/20
摘要： The invention relates to soluble rubella E1 antigens and variants of these antigens. The antigens contain amino acids 201 to 432 or 169 to 432 and are lacking amino acids 453 to 481 as well as at least the amino acids 143 to 164. They further contain a region spanning two disulfide-bridges. The invention also relates to a recombinant DNA molecule encoding the rubella E1 antigens, the expression of rubella E1 antigens as chaperone fusion proteins and their use in a method of detecting antibodies against rubella in a sample.
摘要翻译：本发明涉及可溶性风疹E1抗原和这些抗原的变体。抗原含有氨基酸201至432或169至432，并且缺少氨基酸453至481以及至少氨基酸143至164.它们还包含横跨两个二硫键的区域。本发明还涉及编码风疹E1抗原的重组DNA分子，风疹E1抗原作为伴侣融合蛋白的表达，以及它们在检测样品中风疹抗体的方法中的用途。

3. 发明申请

US20110059552A1 RUBELLA E1 ENVELOPE PROTEIN VARIANTS AND THEIR USE IN DETECTION OF ANTI-RUBELLA ANTIBODIES 有权
标题翻译： RUBELLA E1包膜蛋白变异体及其在检测抗RUBELLA抗体中的应用
公开(公告)号：US20110059552A1
公开(公告)日：2011-03-10
申请号：US12793134
申请日：2010-06-03
申请人： CHRISTIAN SCHOLZ , RALF BOLLHAGEN , ALFRED ENGEL , ELKE FAATZ , PETER SCHAARSCHMIDT , BARBARA UPMEIER , TORALF ZARNT
发明人： CHRISTIAN SCHOLZ , RALF BOLLHAGEN , ALFRED ENGEL , ELKE FAATZ , PETER SCHAARSCHMIDT , BARBARA UPMEIER , TORALF ZARNT
IPC分类号： G01N33/68 , C07K14/19 , C12N9/96 , C07H21/04 , C12N15/63 , C12N1/21 , C12P21/02
CPC分类号： G01N33/56983 , C07K14/005 , C07K2319/35 , C12N2770/36222 , G01N2333/19 , G01N2469/20
摘要： The invention relates to soluble rubella E1 antigens and variants of these antigens. The antigens contain amino acids 201 to 432 or 169 to 432 and are lacking amino acids 453 to 481 as well as at least the amino acids 143 to 164. They further contain a region spanning two disulfide-bridges. The invention also relates to a recombinant DNA molecule encoding the rubella E1 antigens, the expression of rubella E1 antigens as chaperone fusion proteins and their use in a method of detecting antibodies against rubella in a sample.
摘要翻译：本发明涉及可溶性风疹E1抗原和这些抗原的变体。抗原含有氨基酸201至432或169至432，并且缺少氨基酸453至481以及至少氨基酸143至164.它们还包含横跨两个二硫键的区域。本发明还涉及编码风疹E1抗原的重组DNA分子，风疹E1抗原作为伴侣融合蛋白的表达，以及它们在检测样品中风疹抗体的方法中的用途。

4. 发明授权

US09267953B2 Method for detection of specific immunoglobulin class G antibodies 有权
标题翻译：用于检测特异性免疫球蛋白G类抗体的方法
公开(公告)号：US09267953B2
公开(公告)日：2016-02-23
申请号：US12817469
申请日：2010-06-17
申请人： Barbara Upmeier , Ralf Bollhagen , Martina Bronold , Frederic Donie , Christine Markert-Hahn
发明人： Barbara Upmeier , Ralf Bollhagen , Martina Bronold , Frederic Donie , Christine Markert-Hahn
IPC分类号： G01N33/543 , G01N33/68
CPC分类号： G01N33/6854 , G01N33/54313 , G01N33/5438
摘要： Methods for determining an analyte in a sample by immunoassay in a one-step format without performing washing steps are described. The method includes a first analyte-specific receptor that contains at least two binding sites for the analyte, and a second analyte-specific receptor that selectively binds to an aggregate arrangement of at least two analyte molecules bound to the first receptor.
摘要翻译：描述了通过以一步格式进行免疫测定而不执行洗涤步骤来测定样品中分析物的方法。该方法包括含有分析物的至少两个结合位点的第一分析物特异性受体和选择性结合至少两个与第一受体结合的分析物分子的聚集排列的第二分析物特异性受体。

5. 发明申请

US20110143454A1 METHOD FOR DETECTION OF SPECIFIC IMMUNOGLOBULIN CLASS G ANTIBODIES 有权
标题翻译：检测特异性免疫球蛋白G类抗体的方法
公开(公告)号：US20110143454A1
公开(公告)日：2011-06-16
申请号：US12817469
申请日：2010-06-17
申请人： Barbara Upmeier , Ralf Bollhagen , Martina Bronold , Frederic Donie , Christine Markert-Hahn
发明人： Barbara Upmeier , Ralf Bollhagen , Martina Bronold , Frederic Donie , Christine Markert-Hahn
IPC分类号： G01N33/543 , G01N33/566
CPC分类号： G01N33/6854 , G01N33/54313 , G01N33/5438
摘要： The invention relates to a method for determining an analyte in a sample by means of an assay that can be carried out in a one-step format without performing washing steps. The method includes a first analyte-specific receptor that contains at least one bonding point for the analyte, e.g., several antibodies on a particle, as well as a second analyte-specific receptor that can selectively bond to an arrangement comprising at least two analyte molecules which are bound to the first receptor. An antibody sandwich immune assay is described in which the detection is based on electrochemiluminescence.
摘要翻译：本发明涉及通过可以以一步格式执行的测定法来测定样品中分析物的方法，而不进行洗涤步骤。该方法包括第一分析物特异性受体，其包含分析物的至少一个结合点，例如颗粒上的几种抗体，以及可选择性地结合至包含至少两种分析物分子的装置的第二分析物特异性受体它们与第一受体结合。描述了抗体夹心免疫测定法，其中检测基于电化学发光。

6. 发明申请

US20070154883A1 Soluble rubella E1 envelope protein variants 有权
标题翻译：可溶性风疹E1包膜蛋白变体
公开(公告)号：US20070154883A1
公开(公告)日：2007-07-05
申请号：US11584887
申请日：2006-10-23
申请人： Barbara Upmeier , Ralf Bollhagen , Alfred Engel , Elke Faatz , Peter Schaarschmidt , Christian Scholz , Toralf Zarnt
发明人： Barbara Upmeier , Ralf Bollhagen , Alfred Engel , Elke Faatz , Peter Schaarschmidt , Christian Scholz , Toralf Zarnt
IPC分类号： C12Q1/70
CPC分类号： C07K14/005 , C07K2319/35 , C12N2770/36222 , Y10S530/826
摘要： The invention disclosed relates to a soluble rubella E1 antigen and variants of this peptide characterized by lacking at the C-terminal end at least the transmembrane region and the anchor segment as well as at least the amino acids 143 to 164 and containing at least the region spanning the disulfide bridges Cys 349-Cys 352 and Cys 368-401 whereas the N-terminus (Cys 349) of this region contains additionally at least 15 amino acids and/or the C-terminus (Cys 401) of this region contains additionally at least 8 amino acids of the adjacent rubella E1 antigen sequence. Also described are a recombinant DNA molecule encoding the rubella E1 antigen and variants which are recombinantly expressed as a chaperone fusion protein, refolded into a soluble and immunoreactive conformation, and further used for the serological detection of anti-rubella antibodies. In addition, also disclosed is a method for the detection, determination and quantification of anti-rubella antibodies of IgG and/or IgM subclass in a sample wherein the rubella E1 antigen is used as a capture reagent and/or binding partner for the antibodies.
摘要翻译：所公开的本发明涉及可溶性风疹E1抗原和该肽的变体，其特征在于至少在C-末端缺少至少跨膜区和锚段以及至少氨基酸143至164并且至少含有该区域跨越二硫键Cys 349-Cys 352和Cys 368-401，而该区域的N末端（Cys 349）另外包含至少15个氨基酸和/或该区域的C末端（Cys 401）相邻风疹E1抗原序列的至少8个氨基酸。还描述了编码风疹E1抗原的重组DNA分子和重组表达为伴侣融合蛋白的变体，重新折叠成可溶性和免疫反应性构象，并进一步用于抗风疹抗体的血清学检测。此外，还公开了用于检测，测定和定量样品中IgG和/或IgM亚类的抗风疹抗体的方法，其中所述风疹E1抗原用作抗体的捕获试剂和/或结合配偶体。

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