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1. 发明授权

US06821733B2 Methods and compositions for detecting differences between nucleic acids 失效
标题翻译：用于检测核酸之间差异的方法和组合物
公开(公告)号：US06821733B2
公开(公告)日：2004-11-23
申请号：US10071299
申请日：2002-02-07
申请人： Qinghong Yang
发明人： Qinghong Yang
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q1/6839 , C12Q2537/119 , C12Q2521/514
摘要： The present invention provides sensitive methods for detecting the presence or absence of a difference between related nucleic acid sequences. In one aspect of the invention, a method is provided for detecting a difference in the sequence of two nucleic acid molecules. The method includes the steps of: contacting two nucleic acids under conditions that allow the formation of a four-way complex and branch migration; contacting the four-way complex with a tracer molecule and a detection molecule under conditions in which the detection molecule is capable of binding the tracer molecule or the four-way complex; and determining binding of the tracer molecule to the detection molecule before and after exposure to the four-way complex. Competition of the four-way complex with the tracer molecule for binding to the detection molecule indicates a difference between the two nucleic acids. The methods disclosed can be used for detecting nucleotide variations or mutations of an organism, for example, single nucleotide polymorphisms (SNP), which leads to identification of genetic traits associated with diseases or other phenotypic characteristics.
摘要翻译：本发明提供用于检测相关核酸序列之间差异的存在或不存在的敏感方法。在本发明的一个方面，提供了一种检测两个核酸分子的序列差异的方法。该方法包括以下步骤：在允许形成四向复合物和分支迁移的条件下接触两种核酸; 在检测分子能够结合示踪剂分子或四向复合物的条件下，将四元配合物与示踪剂分子和检测分子接触; 以及在暴露于四方复合物之前和之后确定示踪剂分子与检测分子的结合。与检测分子结合的示踪分子的四元复合物的竞争表明两种核酸之间的差异。所公开的方法可用于检测生物体的核苷酸变异或突变，例如单核苷酸多态性（SNP），其导致鉴定与疾病或其他表型特征相关的遗传性状。

2. 发明授权

US08543364B2 Method for optimizing well production in reservoirs having flow barriers 有权
标题翻译：在具有流动障碍的水库中优化井的生产方法
公开(公告)号：US08543364B2
公开(公告)日：2013-09-24
申请号：US12561830
申请日：2009-09-17
申请人： Song Liu , Lixin Tian , Xian-Huan Wen , Chunming Zhao , Qinghong Yang , Peng Zhang , Dengen Zhou , Bo Li , Lichuan Lan , Lizhen Ge , Xinwu Liao , Fengli Zhang , Michael Sheng-Wei Wei
发明人： Song Liu , Lixin Tian , Xian-Huan Wen , Chunming Zhao , Qinghong Yang , Peng Zhang , Dengen Zhou , Bo Li , Lichuan Lan , Lizhen Ge , Xinwu Liao , Fengli Zhang , Michael Sheng-Wei Wei
IPC分类号： G06G7/48
CPC分类号： E21B43/00
摘要： Computer-implemented systems and methods are provided for optimizing hydrocarbon recovery from subsurface formations, including subsurface formations having bottom water or edgewater. A system and method can be configured to receive data indicative of by-pass oil areas in the subsurface formation from reservoir simulation, identify flow barriers in the subsurface formation based on the by-pass oil areas identified by the reservoir simulation, and predict the lateral extension of the identified flow barriers in the subsurface formation. Infill horizontal wells can be placed at areas of the subsurface formation relative to the flow barriers such that production from a horizontal well in the subsurface formation optimizes hydrocarbon recovery.
摘要翻译：提供计算机实现的系统和方法，用于优化地下构造的烃回收，包括具有底部水或污水的地下地层。一种系统和方法可以被配置为接收指示来自储层模拟的地下地层旁路油区的数据，基于由油藏模拟识别的旁路油区，识别地下地层中的流动障碍，并预测横向在地下构造中识别的流动障碍的扩展。填充水平井可以放置在相对于流动屏障的地下地层的区域，使得地下地层中的水平井的生产优化了烃回收。

3. 发明申请

US20100071897A1 METHOD FOR OPTIMIZING WELL PRODUCTION IN RESERVOIRS HAVING FLOW BARRIERS 有权
标题翻译：在流动障碍物储层中优化生产的方法
公开(公告)号：US20100071897A1
公开(公告)日：2010-03-25
申请号：US12561830
申请日：2009-09-17
申请人： Song Liu , Lixin Tian , Xian-Huan Wen , Chunming Zhao , Qinghong Yang , Peng Zhang , Dengen Zhou , Bo Li , Lichuan Lan , Lizhen Ge , Xinwu Liao , Fengli Zhang , Michael Sheng-Wei Wei
发明人： Song Liu , Lixin Tian , Xian-Huan Wen , Chunming Zhao , Qinghong Yang , Peng Zhang , Dengen Zhou , Bo Li , Lichuan Lan , Lizhen Ge , Xinwu Liao , Fengli Zhang , Michael Sheng-Wei Wei
IPC分类号： E21B43/00 , E21B47/00
CPC分类号： E21B43/00
摘要： Computer-implemented systems and methods are provided for optimizing hydrocarbon recovery from subsurface formations, including subsurface formations having bottom water or edgewater. A system and method can be configured to receive data indicative of by-pass oil areas in the subsurface formation from reservoir simulation, identify flow barriers in the subsurface formation based on the by-pass oil areas identified by the reservoir simulation, and predict the lateral extension of the identified flow barriers in the subsurface formation. Infill horizontal wells can be placed at areas of the subsurface formation relative to the flow barriers such that production from a horizontal well in the subsurface formation optimizes hydrocarbon recovery.
摘要翻译：提供计算机实现的系统和方法，用于优化地下构造的烃回收，包括具有底部水或污水的地下地层。一种系统和方法可以被配置为接收指示来自储层模拟的地下地层旁路油区的数据，基于由油藏模拟识别的旁路油区，识别地下地层中的流动障碍，并预测横向在地下构造中识别的流动障碍的扩展。填充水平井可以放置在相对于流动屏障的地下地层的区域，使得地下地层中的水平井的生产优化了烃回收。

4. 发明授权

US06878530B2 Methods for detecting polymorphisms in nucleic acids 失效
标题翻译：检测核酸多态性的方法
公开(公告)号：US06878530B2
公开(公告)日：2005-04-12
申请号：US10071302
申请日：2002-02-07
申请人： Qinghong Yang
发明人： Qinghong Yang
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6858 , C12Q2525/313 , C12Q2525/161
摘要： The present invention provides methods for determining the genotype of a nucleic acid at the site of a polymorphism. The methods achieve sensitivities great enough to detect the presence of any difference between the nucleic acids, even single nucleotide polymorphisms. In the methods, the nucleic acid is compared to a reference nucleic acid having a known genotype. The nucleic acids can be of any length, even less than 100 base pairs. In methods, one or more extra mismatches are introduced into the nucleic acids at or near the site of the polymorphism. The nucleic acids are contacted under conditions in which they are capable of forming a stable four-way complex that can be detected to indicate that the nucleic acids differ in genotype.
摘要翻译：本发明提供了确定多态性位点处核酸基因型的方法。该方法实现足够大的灵敏度以检测核酸之间的任何差异，甚至单核苷酸多态性的存在。在该方法中，将核酸与具有已知基因型的参照核酸进行比较。核酸可以是任何长度，甚至小于100个碱基对。在方法中，将一个或多个额外的错配引入多态性位点处或附近的核酸中。核酸在能够形成稳定的四向复合物的条件下接触，其可被检测以表明核酸在基因型上不同。

5. 发明授权

US06653079B2 Methods for detection of differences in nucleic acids 失效
公开(公告)号：US06653079B2
公开(公告)日：2003-11-25
申请号：US09804661
申请日：2001-03-12
申请人： Qinghong Yang , Wendy Yang , Alla Lishanski
发明人： Qinghong Yang , Wendy Yang , Alla Lishanski
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , Y10T436/143333 , C12Q2525/313
摘要： The present invention provides methods for detecting the presence or absence of a difference between two related nucleic acid sequences. In the methods, a target nucleic acid and a reference nucleic acid are contacted under conditions in which they are capable of forming a four-way nucleic acid complex with a branch structure that is capable of migration. Under the contact conditions, if the reference nucleic acid and target nucleic acid are identical, branch migration is capable of going to completion resulting in complete strand exchange. If the reference nucleic acid and target nucleic acid are different, branch migration does not go to completion, resulting in a stable four-way complex. Detection of the stable four-way complex identifies the presence of a difference between the nucleic acids. A stable four-way complex can be detected with molecules that specifically bind such complexes, by gel electrophoresis or by specific isolation of the stable four-way complex.

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