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1. 发明授权

US5019231A Electro-blotting of electrophoretically resolved fluroescent-labeled saccharides and detection of active structures with protein probes 失效
标题翻译：电泳分辨荧光标记的糖的电泳印迹和蛋白质探针活性结构的检测
公开(公告)号：US5019231A
公开(公告)日：1991-05-28
申请号：US481367
申请日：1990-02-16
申请人： Brian K. Brandley , Paul G. James , Michael Tiemeyer
发明人： Brian K. Brandley , Paul G. James , Michael Tiemeyer
IPC分类号： C07H15/203 , G01N33/561 , G01N33/66
CPC分类号： C07H15/203 , G01N33/561 , G01N33/66
摘要： Saccharide in mixtures are separated into groups or individual saccharides and tested for their affinity for particular proteins. The method of the invention is carried out by conjugating saccharides in a mixture with 4-amino-1-naphthalene sulfonic acid (ANSA) to form conjugates. The saccharide/ANSA conjugates are subjected to electrophoretic resolution within gels. The resolved bands of material are electro-blotted onto charged nylon membranes to provide a stable record of the electrophoretic separation. The blots on the nylon membranes are brought into contact with particular proteins in order to determine the binding affinity of these particular proteins to particular saccharides on the nylon membrane. The proteins are preferably bound to labels so that the binding of the proteins to the saccharides can be easily detected.
摘要翻译：将混合物中的糖分离成组或单独的糖，并测试其对特定蛋白质的亲和力。本发明的方法通过将糖与4-氨基-1-萘磺酸（ANSA）混合形成共轭物来进行。糖/ ANSA缀合物在凝胶内进行电泳分辨。将解析的材料带电印到带电尼龙膜上以提供电泳分离的稳定记录。使尼龙膜上的印迹与特定蛋白质接触，以确定这些特定蛋白质对尼龙膜上特定糖的结合亲和力。蛋白质优选与标记物结合，使得可以容易地检测蛋白质与糖的结合。

2. 发明授权

US08157016B2 Fluid metering device and method for well tool 有权
标题翻译：流体计量装置和井工具的方法
公开(公告)号：US08157016B2
公开(公告)日：2012-04-17
申请号：US12390758
申请日：2009-02-23
申请人： Paul G. James
发明人： Paul G. James
IPC分类号： E21B34/10
CPC分类号： E21B23/04 , E21B34/10 , F15B11/13 , F15B2211/405 , F15B2211/455
摘要： A method of actuating a well tool includes the steps of: increasing pressure in a fluid line of a fluid metering device; closing a pilot-operated valve in response to the pressure increasing step; and discharging a predetermined volume of fluid from the metering device in response to the pressure increasing and valve closing steps. A fluid metering device for a well tool includes a piston separating chambers; and a pilot-operated valve which selectively prevents fluid communication between the chambers in response to at least a predetermined pressure being applied to a fluid line of the metering device, and which permits fluid communication through the valve between the chambers in response to pressure in the fluid line being less than the predetermined pressure. A greater pressure may be applied to the fluid line to thereby bypass the metering device and allow fluid to discharge from the line.
摘要翻译：致动井工具的方法包括以下步骤：增加流体计量装置的流体管线中的压力; 响应于所述增压步骤关闭先导阀; 并响应于增压和关阀步骤从计量装置排出预定体积的流体。用于井具的流体计量装置包括活塞分离室; 以及先导式操作阀，其选择性地防止响应于至少预定压力施加到计量装置的流体管线的腔室之间的流体连通，并且其允许通过响应于所述计量装置中的压力而在腔室之间的阀的流体连通流体管线小于预定压力。可以将更大的压力施加到流体管线，从而绕过计量装置并允许流体从管线排出。

3. 发明授权

US5094731A Electro-blotting of electrophoretically resolved fluorescent-labeled saccharides and detection of active structures with protein probes 失效
标题翻译：电泳分解荧光标记物和用蛋白质探针检测活性结构
公开(公告)号：US5094731A
公开(公告)日：1992-03-10
申请号：US589431
申请日：1990-09-27
申请人： Brian K. Brandley , Paul G. James , Michael Tiemeyer
发明人： Brian K. Brandley , Paul G. James , Michael Tiemeyer
IPC分类号： C07H15/203 , G01N33/543 , G01N33/561 , G01N33/58 , G01N33/66
CPC分类号： C07H15/203 , G01N33/54306 , G01N33/561 , G01N33/582 , G01N33/66
摘要： Saccharide in mixtures are separated into groups or individual saccharides and tested for their affinity for particular proteins. The method of the invention is carried out by conjugating saccharides in a mixture with charge-generating moieties and moieties capable of fluorescing such as 4-amino-1-naphthalene sulfonic acid (ANSA) to form conjugates. The saccharide conjugates are subjected to electrophoretic resolution within gels. The resolved bands of material can be seen under ultraviolet light and are electro-blotted onto charged nylon membranes to provide a stable record of the electrophoretic separation. The blots (visible under ultraviolet light) on the nylon membranes are brought into contact with particular proteins in order to determine the binding affinity of these particular proteins to particular saccharides on the nylon membrane. The proteins are preferably bound to labels so that the binding of the proteins to the saccharides can be easily detected.
摘要翻译：将混合物中的糖分离成组或单独的糖，并测试其对特定蛋白质的亲和力。本发明的方法通过将糖与电荷产生部分和能够发荧光的部分如4-氨基-1-萘磺酸（ANSA）结合成混合物来进行，以形成共轭物。糖胶体在凝胶内进行电泳分解。可以在紫外光下观察到解析的材料带，并将其电印到带电尼龙膜上，以提供电泳分离的稳定记录。使尼龙膜上的印迹（在紫外光下可见）与特定蛋白质接触，以确定这些特定蛋白质对尼龙膜上特定糖的结合亲和力。蛋白质优选与标记物结合，使得可以容易地检测蛋白质与糖的结合。

4. 外观设计

USD741688S1 Bracket with slotted side walls 有权
标题翻译：带开槽侧壁的支架
公开(公告)号：USD741688S1
公开(公告)日：2015-10-27
申请号：US29443887
申请日：2013-01-23
申请人： Paul G. James
设计人： Paul G. James

5. 发明申请

US20100212910A1 FLUID METERING DEVICE AND METHOD FOR WELL TOOL 有权
标题翻译：流体测量装置和方法
公开(公告)号：US20100212910A1
公开(公告)日：2010-08-26
申请号：US12390758
申请日：2009-02-23
申请人： Paul G. JAMES
发明人： Paul G. JAMES
IPC分类号： E21B34/06 , E21B34/00
CPC分类号： E21B23/04 , E21B34/10 , F15B11/13 , F15B2211/405 , F15B2211/455
摘要： A method of actuating a well tool includes the steps of: increasing pressure in a fluid line of a fluid metering device; closing a pilot-operated valve in response to the pressure increasing step; and discharging a predetermined volume of fluid from the metering device in response to the pressure increasing and valve closing steps. A fluid metering device for a well tool includes a piston separating chambers; and a pilot-operated valve which selectively prevents fluid communication between the chambers in response to at least a predetermined pressure being applied to a fluid line of the metering device, and which permits fluid communication through the valve between the chambers in response to pressure in the fluid line being less than the predetermined pressure. A greater pressure may be applied to the fluid line to thereby bypass the metering device and allow fluid to discharge from the line.
摘要翻译：致动井工具的方法包括以下步骤：增加流体计量装置的流体管线中的压力; 响应于所述增压步骤关闭先导阀; 并响应于增压和关阀步骤从计量装置排出预定体积的流体。用于井具的流体计量装置包括活塞分离室; 以及先导式操作阀，其选择性地防止响应于至少预定压力施加到计量装置的流体管线的腔室之间的流体连通，并且其允许通过响应于所述计量装置中的压力而在腔室之间的阀的流体连通流体管线小于预定压力。可以将更大的压力施加到流体管线，从而绕过计量装置并允许流体从管线排出。

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