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    • 3. 发明授权
    • Method for the direct detection of methicillin-resistant staphylococcus aureus
    • 直接检测耐甲氧西林金黄色葡萄球菌的方法
    • US07955796B2
    • 2011-06-07
    • US10471819
    • 2002-03-14
    • Jacques SchrenzelPatrice Francois
    • Jacques SchrenzelPatrice Francois
    • C12Q1/68
    • G01N33/56938C12Q1/14C12Q1/689C12Q2600/16
    • Described is a method for the detection and quantification of methicillin-resistant Staphylococcus aureus from a specimen comprising a) contacting the specimen with Anti-Protein A antibodies so as to adsorb methicillinresistant Staphylococcus aureus and/or methicillin-sensitive Staphylococcus aureus, b) separating said antibodies to which methicillin-resistant Staphylococcus aureus and/or methicillin-sensitive Staphylococcus aureus have been adsorbed from the specimen, c) lysing methicillin-resistant Staphylococcus aureus and/or methicillin-sensitive Staphylococcus aureus adsorbed to said antibodies so as to release their DNA, d) combining the released DNA with (i) probes and/or primers which are specific for a target DNA sequence of the mecA gene of methicillin -resistant Staphylococcus aureus and/or of methicillin-resistant Staphylococcus epidermis, (ii) probes and/or primers which are specific for a target DNA sequence of methicillin-resistant Staphylococcus aureus other than a target DNA sequence of the mecA gene and (iii) probes and/or primers which are specific for a target DNA sequence of methicillin-resistant Staphylococcus epidermis other than a target DNA sequence of the mecA gene, whereby the target DNA sequences of methicillin-resistant Staphylococcus aureus and of methicillin-resistant Staphylococcus epidermis are not homologous, e) subjecting the combined released DNA and the specific probes and/or primers to conditions which permit amplification of said target DNA sequences, f) detecting the presence and amount of the amplified target DNA sequences as an indication of the presence and amount of methicillin-resistant Staphylococcus aureus, new primers and probes and diagnostic kits for the detection and quantification of MRSA.
    • 描述了一种用于从标本检测和定量耐甲氧西林金黄色葡萄球菌的方法,包括a)使样品与抗蛋白A抗体接触,以吸附耐甲氧西林金黄色葡萄球菌和/或甲氧西林敏感性金黄色葡萄球菌,b)将所述 从样品中吸附了耐甲氧西林金黄色葡萄球菌和/或甲氧西林敏感性金黄色葡萄球菌的抗体,c)溶解吸附在所述抗体上以释放其DNA的耐甲氧西林金黄色葡萄球菌和/或甲氧西林敏感性金黄色葡萄球菌, d)将释放的DNA与(i)针对耐甲氧西林金黄色葡萄球菌和/或耐甲氧西林金黄色葡萄球菌的mecA基因的靶DNA序列特异的探针和/或引物组合,(ii)探针和/或 针对甲氧西林耐药金黄色葡萄球菌以外的靶标DNA序列特异的引物 和mecA基因的DNA序列,以及(iii)除了mecA基因的靶DNA序列以外,对甲氧西林抗性葡萄球菌表皮特异性的探针和/或引物,其中耐甲氧西林的靶DNA序列 金黄色葡萄球菌和耐甲氧西林葡萄球菌表皮不同源,e)使合并的释放的DNA和特异性探针和/或引物经受允许扩增所述靶DNA序列的条件,f)检测扩增靶的存在和量 DNA序列作为甲氧西林耐药金黄色葡萄球菌的存在和数量的指示,新的引物和探针以及用于检测和定量MRSA的诊断试剂盒。