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1. 发明授权

US6413586B2 Apparatus and method for spotting a substrate 有权
公开(公告)号：US6413586B2
公开(公告)日：2002-07-02
申请号：US81296101
申请日：2001-03-20
申请人： PE CORP NY
发明人： VANN CHARLES S , BRYNING ZBIGNIEW
IPC分类号： G01N37/00 , B01J19/00 , B01L3/02 , C40B60/14 , G01N35/00 , G01N35/10 , B05D1/30
CPC分类号： B01L3/0244 , B01J19/0046 , B01J2219/00369 , B01J2219/00376 , B01J2219/00387 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00689 , B01L3/0251 , B01L3/0262 , B01L2300/0819 , B01L2300/0838 , B01L2400/028 , B01L2400/0406 , C40B60/14 , G01N35/1016 , G01N35/1067 , G01N35/1074 , G01N2035/00237 , G01N2035/1034 , G01N2035/1037 , G01N2035/1039 , G01N2035/1069 , Y10T436/25 , Y10T436/2575
摘要： The present invention provides a method and apparatus for dispensing a small volume of a selected liquid, such as a biological sample or reagent, onto a substrate. The device includes a tube adapted to contain the liquid. An elongate fiber is disposed within the tube for axial movement therein between raised and lowered positions. Upon shifting or oscillating the fiber between its raised and lowered positions, a liquid spot can be formed at a selected position on the substrate. The device is readily adaptable for the production of micro-arrays having a great number of individual spots.

2. 发明授权

US6316610B2 Labelled oligonucleotides synthesized on solid-supports 有权
标题翻译：在固体支持物上合成标记的寡核苷酸
公开(公告)号：US6316610B2
公开(公告)日：2001-11-13
申请号：US81337801
申请日：2001-03-20
申请人： PE CORP NY
发明人： LEE LINDA G , MULLAH KHAIRUZZAMAN B , ROSENBLUM BARNETT B
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/68 , C07H19/00 , C07H21/02 , C07H21/04
CPC分类号： C07H21/00 , C07H19/10 , C07H19/20 , Y02P20/55
摘要： Methods and compositions to label oligonucleotides and analogs directly on a solid-support having the structurewhere S is a solid-support, A is a cleavable linker, X is a moiety with three or more attachment sites, L is a label, Y is a nucleophile, i.e. O, NH, NR or S, and P1 is an acid cleavable protecting group are provided. The labelled solid-support is reacted in a cyclical fashion to synthesize a labelled oligonucleotide on a solid-support in the 5' to 3' direction, having the structure:Labelled oligonucleotides are also synthesized by reacting: (i) a label reagent bearing functionality consisting of carboxylic acid, sulfonic acid, phosphonic acid, or phosphoric acid, (ii) an oligonucleotide on solid support with nucleophilic functionality, and (iii) a coupling reagent, whereby an ester, amide, thioester, sulfonamide, sulfonate, phosphonate, phosphoramidate, phosphorothioate, or phosphate bond is formed. The labelling reaction may be conducted at label sites including the 5' terminus, the 3' terminus, a nucleobase, an internucleotide linkage, a sugar, amino, sulfide, hydroxyl, and carboxyl.
摘要翻译：方法和组合物直接在具有其中S是固体支持物的结构的固体支持物上标记寡核苷酸和类似物，A是可切割的接头，X是具有三个或更多个连接位点的部分，L是标记，Y是亲核试剂，即O，NH，NR或S，并且P1是可酸切割的保护基。标记的固体支持物以循环方式反应以在5'至3'方向的固体支持物上合成标记的寡核苷酸，具有以下结构：标记的寡核苷酸还通过以下方式合成：（i）标记试剂载体官能团由羧酸，磺酸，膦酸或磷酸组成，（ii）具有亲核官能团的固体支持物上的寡核苷酸，和（iii）偶联剂，其中酯，酰胺，硫酯，磺酰胺，磺酸酯，膦酸酯，氨基磷酸酯，硫代磷酸酯或磷酸酯键。标记反应可以在包括5'末端，3'末端，核碱基，核苷酸间键，糖，氨基，硫化物，羟基和羧基的标记位点进行。

3. 发明授权

US6440217B2 Apparatus and method for spotting a substrate 有权
公开(公告)号：US6440217B2
公开(公告)日：2002-08-27
申请号：US81264301
申请日：2001-03-20
申请人： PE CORP NY
发明人： VANN CHARLES S , BRYNING ZBIGNIEW
IPC分类号： G01N37/00 , B01J19/00 , B01L3/02 , C40B60/14 , G01N35/00 , G01N35/10 , B05C5/00
CPC分类号： B01L3/0244 , B01J19/0046 , B01J2219/00369 , B01J2219/00376 , B01J2219/00387 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00689 , B01L3/0251 , B01L3/0262 , B01L2300/0819 , B01L2300/0838 , B01L2400/028 , B01L2400/0406 , C40B60/14 , G01N35/1016 , G01N35/1067 , G01N35/1074 , G01N2035/00237 , G01N2035/1034 , G01N2035/1037 , G01N2035/1039 , G01N2035/1069 , Y10T436/25 , Y10T436/2575
摘要： The present invention provides a method and apparatus for dispensing a small volume of a selected liquid, such as a biological sample or reagent, onto a substrate. The device includes a tube adapted to contain the liquid. An elongate fiber is disposed within the tube for axial movement therein between raised and lowered positions. Upon shifting or oscillating the fiber between its raised and lowered positions, a liquid spot can be formed at a selected position on the substrate. The device is readily adaptable for the production of micro-arrays having a great number of individual spots.

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