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1. 发明授权

US5789165A Method and reagent for simultaneously assaying one or more ligands in a group of preselected ligands 失效
标题翻译：用于同时测定一组预选配体中的一种或多种配体的方法和试剂
公开(公告)号：US5789165A
公开(公告)日：1998-08-04
申请号：US535248
申请日：1995-10-30
申请人： Yuichi Oku , Noriko Toyoda
发明人： Yuichi Oku , Noriko Toyoda
IPC分类号： C12Q1/68 , G01N33/543
CPC分类号： C12Q1/6804 , G01N33/543 , Y10S435/962 , Y10S435/971 , Y10S435/973 , Y10S435/975 , Y10S436/808 , Y10S436/824
摘要： The assay reagent and kit of the present invention suppress non-specific binding of a labeled substance onto a solid phase, and can assay one or more species of antibodies or one or more species of antigens by means of a single reagent in a simple manner. The assay method involves reacting immunological ligands in a test sample with the assay reagent which contains a combination of components (A) and (B), thereby forming complexes, which complexes are captured onto the independently and separately present Solid phases (C), to assay the label contained in the complexes. Component (A): one or more species of immunological anti-ligand-nucleotide conjugates, in each of which nucleotides with a specific base sequence, independently selected depending on the species of an immunological ligand, are bound to an immunological anti-ligand having a specific immunological affinity to one of the different species of immunological ligands to be assayed; (B): labeled substances each having a specific affinity to one of the different species of immunological ligands to be assayed; Solid phase (C): solid phase-nucleotide conjugates wherein nucleotides, each having a base sequence complementarily binding to a nucleotide of the above component (A), are immobilized onto a water-insoluble support. Either an antigen or an antibody may serve as the aforementioned immunological ligand and immunological anti-ligand.
摘要翻译： PCT No.PCT / JP94 / 00725 Sec。 371 1995年10月30日第 102（e）日期1995年10月30日PCT 1994年4月28日PCT PCT。公开号WO94 / 27150 日期1994年11月24日本发明的测定试剂和试剂盒抑制标记物质在固相上的非特异性结合，并且可以通过单一试剂测定一种或多种抗体或一种或多种抗原以简单的方式。测定方法包括使测试样品中的免疫配体与含有组分（A）和（B）的组合的测定试剂反应，从而形成复合物，该复合物被捕获到独立和单独存在的固相（C）上，测定复合物中所含的标记。组分（A）：一种或多种免疫抗配体 - 核苷酸缀合物，其中每一种具有特定碱基序列的核苷酸根据免疫配体的种类而独立地选择，其结合于具有对待测定的不同种类的免疫配体之一的特异性免疫亲和力; （B）：各种与被测定的不同种类的免疫配体之一具有特异性亲和力的标记物质; 固相（C）：固相 - 核苷酸偶联物，其中各自具有与上述组分（A）的核苷酸互补结合的碱基序列的核苷酸固定在水不溶性载体上。抗原或抗体可以用作上述免疫配体和免疫抗配体。

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