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1. 发明授权

US08465920B2 Method for hybridizing nucleic acids 有权
标题翻译：核酸杂交方法
公开(公告)号：US08465920B2
公开(公告)日：2013-06-18
申请号：US12735261
申请日：2008-09-12
申请人： Nathalie Lenne , Patrick Erbacher
发明人： Nathalie Lenne , Patrick Erbacher
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6832 , C12Q2525/113 , C12Q2563/113 , C12Q2525/119
摘要： The invention relates to a method for manipulating, isolating, detecting or amplifying a target nucleic acid in a sample by hybridization with an oligonucleotide-oligocation conjugate, comprising allowing said nucleic acid to react with an oligonucleotide-oligocation conjugate comprising at least A1 and Bj linked together directly or via a linker, wherein. A, is an i-mer oligonucleotides, with i=3 to 50, where Ai is an oligomer with naturally or non naturally occurring nucleobases and/or pentafuranosyl groups and/or native phosphodiester bonds, optionally comprising a marker group. Bj is a j-mer organic oligocation moiety, with j=1 to 50, where B is —HPO3—R1—(NH—R2)n—NH—R3—O—, where R1, R2 and R3 are lower alkylene, identical or different, NH—R2 moieties being identical or different when n is >1; HPO3—R1—CH(X)—R3—O—, where Ri and R3, identical or different, are lower alkylene and X is putrescine, spermidine or spermine residue.
摘要翻译：本发明涉及通过与寡核苷酸寡核苷酸缀合物杂交来操纵，分离，检测或扩增样品中的靶核酸的方法，包括使所述核酸与包含至少A1和Bj连接的寡核苷酸寡核苷酸轭合物反应一起直接或通过连接体，其中。 A是i = 3至50的i-mer寡核苷酸，其中A 1是具有天然或非天然存在的核碱基和/或五五烷酰基基团和/或天然磷酸二酯键的寡聚体，任选地包含标记基团。 Bj是j-1有机寡核苷酸部分，其中B = -HPO 3 -R1-（NH-R 2）n -NH-R 3 -O-，其中R 1，R 2和R 3为低级亚烷基，相同或不同的NH-R 2部分在n> 1时相同或不同; HPO 3 -R 1 -CH（X）-R 3 -O-，其中R 1和R 3相同或不同，为低级亚烷基，X为腐胺，亚精胺或精胺残基。

2. 发明申请

US20100311056A1 METHOD FOR HYBRIDIZING NUCLEIC ACIDS 有权
标题翻译：用于杂交核酸的方法
公开(公告)号：US20100311056A1
公开(公告)日：2010-12-09
申请号：US12735261
申请日：2008-09-12
申请人： Nathalie Lenne , Patrick Erbacher
发明人： Nathalie Lenne , Patrick Erbacher
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6832 , C12Q2525/113 , C12Q2563/113 , C12Q2525/119
摘要： The invention relates to a method for manipulating, isolating, detecting or amplifying a target nucleic acid in a sample by hybridization with an oligonucleotide-oligocation conjugate, comprising allowing said nucleic acid to react with an oligonucleotide-oligocation conjugate comprising at least A1 and Bj linked together directly or via a linker, wherein. A, is an i-mer oligonucleotides, with i=3 to 50, where Ai is an oligomer with naturally or non naturally occurring nucleobases and/or pentafuranosyl groups and/or native phosphodiester bonds, optionally comprising a marker group. Bj is a j-mer organic oligocation moiety, with j=1 to 50, where B is —HPO3—R1—(NH—R2)n—NH—R3—O—, where R1, R2 and R3 are lower alkylene, identical or different, NH—R2 moieties being identical or different when n is >1; HPO3—R1—CH(X)—R3—O—, where Ri and R3, identical or different, are lower alkylene and X is putrescine, spermidine or spermine residue.
摘要翻译：本发明涉及通过与寡核苷酸寡聚轭合物杂交来操纵，分离，检测或扩增样品中的靶核酸的方法，包括使所述核酸与至少包含A1和Bj连接的寡核苷酸寡聚轭合物反应一起直接或通过连接体，其中。 A是i = 3至50的i-mer寡核苷酸，其中A 1是具有天然或非天然存在的核碱基和/或五五烷酰基基团和/或天然磷酸二酯键的寡聚体，任选地包含标记基团。 Bj是j-1有机寡核苷酸部分，其中B = -HPO 3 -R1-（NH-R 2）n -NH-R 3 -O-，其中R 1，R 2和R 3为低级亚烷基，相同或不同的NH-R 2部分在n> 1时相同或不同; HPO 3 -R 1 -CH（X）-R 3 -O-，其中R 1和R 3相同或不同，为低级亚烷基，X为腐胺，亚精胺或精胺残基。

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