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    • 3. 发明申请
    • Method of identification and cloning differentially expressed messenger RNAs
    • 识别和克隆差异表达信使RNA的方法
    • US20050191666A1
    • 2005-09-01
    • US11031077
    • 2005-01-10
    • Alexander BelyavskyNatalia Ivanova
    • Alexander BelyavskyNatalia Ivanova
    • C07H21/02C07H21/04C12N15/10C12P19/34C12Q1/68C12Q1/6809C12Q1/6855
    • C12N15/1072C12N15/1096C12Q1/6809C12Q1/6855
    • A method of identification of differentially expressed messenger RNA (mRNA) which consists of synthesizing from a set of sequences of mRNA sets of fragments of complementary DNA (cDNA), which are separated with the aid of gel electrophoresis and the pictures of separation of the cDNA from different types of cells are compared and fragments with differential signal intensity are identified. For formation of the set of fragments the cDNA is cleaved with the aid of restriction nucleases. A method of cloning of differentially expressed mRNAs consists of synthesizing from sets of sequences of mRNAs from different types of cells sets of fragments of complementary DNA (cDNA) which are separated with the aid of gel electrophoresis, the pictures of the separation of the cDNA from different types of cells are compared, fragments of cDNA with different signal intensities are separated from the gel, amplified with the aid of a polymerase chain reaction and cloned to a plasmid or phage vector. For the formation of the set of fragments one carried out cleavage of the cDNA with the aid of restriction endonucleases and uses only those fragments of cDNA that correspond to the 3′ or 5′ and regions of the mRNAs.
    • 鉴别差异表达的信使RNA(mRNA)的方法,其由通过凝胶电泳分离的互补DNA(cDNA)片段的mRNA集合的一组序列合成而组合,以及分离cDNA的图片 比较来自不同类型的细胞,并鉴定具有差异信号强度的片段。 为了形成该组片段,借助于限制性核酸酶将cDNA切割。 克隆差异表达的mRNA的方法包括从借助于凝胶电泳分离的来自不同类型的细胞组的互补DNA片段(cDNA)的mRNA集合的集合的合成,将cDNA从 比较不同类型的细胞,从凝胶中分离具有不同信号强度的cDNA片段,借助于聚合酶链反应扩增,并克隆到质粒或噬菌体载体中。 为了形成一组片段,一个在限制性内切核酸酶的帮助下进行cDNA的切割,并且仅使用对应于mRNA的3'或5'和区域的那些cDNA片段。
    • 4. 发明申请
    • METHOD AND SYSTEM FOR ACTIVATION
    • 用于激活的方法和系统
    • US20130290714A1
    • 2013-10-31
    • US13459331
    • 2012-04-30
    • Sebastian HansAlexander GlasmanNatalia Ivanova
    • Sebastian HansAlexander GlasmanNatalia Ivanova
    • H04L9/28
    • G06F21/34G06F21/445H04L9/3273H04L2463/062H04W12/06
    • An activation method includes a security module card verifying an activation server using a shared secret key and a first challenge, mediating a verification of the activation server by a mobile device, and encrypting the second challenge using a generated key to obtain an encrypted second challenge. The security module card further transmits the encrypted second challenge and a third challenge to the mobile device. The method further includes receiving an encrypted third challenge from the mobile device, decrypting the encrypted third challenge to obtain a received third challenge, verifying the mobile device based on the received third challenge being equal to the third challenge sent to the mobile device, and sending a validation of mutual trust between the security module card and the mobile device to the activation server.
    • 激活方法包括使用共享秘密密钥和第一挑战来验证激活服务器的安全模块卡,通过移动设备介入激活服务器的验证,以及使用生成的密钥对第二挑战进行加密以获得加密的第二挑战。 安全模块卡进一步向移动设备发送加密的第二挑战和第三挑战。 该方法还包括从移动设备接收加密的第三挑战,解密加密的第三挑战以获得接收到的第三挑战,基于接收到的第三挑战等于发送到移动设备的第三挑战来验证移动设备,以及发送 安全模块卡和移动设备之间的互信验证到激活服务器。
    • 6. 发明授权
    • Device and method for pumping fluids employing the movement of gas bubbles in microscale
    • 采用微气泡气泡移动的装置和方法
    • US07942643B2
    • 2011-05-17
    • US11006650
    • 2004-12-08
    • Hye-jung ChoNatalia Ivanova
    • Hye-jung ChoNatalia Ivanova
    • F04B19/24F04B1/18
    • F04B19/24F04B19/006
    • The present fluid pumping method for micro-fluidic devices uses gas bubbles to move fluid by light beams. The light beams are emitted to the fluid near the gas bubble through an optically transparent cover and correspondingly heat the fluid in the micro channels. The liquid temperature variation changes the surface tension of the gas bubble near the heated fluid side, therefore, a pressure gradient between the end portions of the gas bubble generates accordingly. By moving the light beams, the moved pressure difference will be achieved, which will drive the gas bubbles and pump the fluid. Such a fluid pumping can simplify the structure of a micro-fluidic device and eliminate heat loss because of using a controllable light beam.
    • 用于微流体装置的本液体泵送方法使用气泡来通过光束移动流体。 光束通过光学透明盖发射到气泡附近的流体,并相应地加热微通道中的流体。 液体温度变化改变加热流体侧附近的气泡的表面张力,因此气泡的端部之间的压力梯度相应地产生。 通过移动光束,将实现移动的压力差,这将驱动气泡并泵送流体。 这种流体泵送可以简化微流体装置的结构,并且由于使用可控光束而消除热损失。
    • 7. 发明授权
    • Method and system for activation
    • 激活方法和系统
    • US08607050B2
    • 2013-12-10
    • US13459331
    • 2012-04-30
    • Sebastian HansAlexander GlasmanNatalia Ivanova
    • Sebastian HansAlexander GlasmanNatalia Ivanova
    • H04L9/32
    • G06F21/34G06F21/445H04L9/3273H04L2463/062H04W12/06
    • An activation method includes a security module card verifying an activation server using a shared secret key and a first challenge, mediating a verification of the activation server by a mobile device, and encrypting the second challenge using a generated key to obtain an encrypted second challenge. The security module card further transmits the encrypted second challenge and a third challenge to the mobile device. The method further includes receiving an encrypted third challenge from the mobile device, decrypting the encrypted third challenge to obtain a received third challenge, verifying the mobile device based on the received third challenge being equal to the third challenge sent to the mobile device, and sending a validation of mutual trust between the security module card and the mobile device to the activation server.
    • 激活方法包括使用共享秘密密钥和第一挑战来验证激活服务器的安全模块卡,通过移动设备介入激活服务器的验证,以及使用生成的密钥对第二挑战进行加密以获得加密的第二挑战。 安全模块卡进一步向移动设备发送加密的第二挑战和第三挑战。 该方法还包括从移动设备接收加密的第三挑战,解密加密的第三挑战以获得接收到的第三挑战,基于接收到的第三挑战等于发送到移动设备的第三挑战来验证移动设备,以及发送 安全模块卡和移动设备之间的互信验证到激活服务器。