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2. 发明授权

US08062845B2 Rapid nucleic acid isolation method and compositions 有权
标题翻译：快速核酸分离方法和组合物
公开(公告)号：US08062845B2
公开(公告)日：2011-11-22
申请号：US10565358
申请日：2004-07-21
申请人： Ashfaque Hossain , Nancy Jo Dohse Hanson , Mark D. Reisbig
发明人： Ashfaque Hossain , Nancy Jo Dohse Hanson , Mark D. Reisbig
IPC分类号： C12Q1/68
CPC分类号： C12N15/1003
摘要： A method of isolating RNA from a biological specimen is provided, whereby a biological specimen is contacted with an admixture of (i) a mono-phasic solution of phenol and guanidine isothiocyanate and (ii) a lysis buffer under conditions and for a time appropriate to form a homogenate. Next, the homogenate is admixed with a water-immiscible organic solvent under conditions and for a time appropriate to form an aqueous phase and an organic phase. The aqueous phase is then contacted with a C1-C4 lower alcohol under conditions and for a time to form a precipitated RNA. The precipitated RNA is then recovered by centrifugation and decanting of the aqueous phase. The method can also be used to isolate total RNA. In an alternative embodiment, the biological sample is contacted with (i) a lysis buffer, and (ii) a mono-phasic solution of phenol and guanidine isothiocyanate under conditions and for a time appropriate to form a homogenate. The remaining steps of this embodiment are the same as above.
摘要翻译：提供了从生物标本中分离RNA的方法，由此将生物样品与（i）苯酚的一相溶液和异硫氰酸胍的混合物和（ii）裂解缓冲液的混合物接触，条件和时间适于形成匀浆。接下来，在适于形成水相和有机相的条件下，将匀浆物与水不混溶的有机溶剂混合。然后将水相与C1-C4低级醇在条件下和一段时间内接触形成沉淀的RNA。然后通过离心和倾析水相回收沉淀的RNA。该方法也可用于分离总RNA。在替代实施方案中，将生物样品与（i）裂解缓冲液和（ii）苯酚和异硫氰酸胍的一步溶液在适当的条件下进行接触以形成匀浆。本实施例的其余步骤与上述相同。

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