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1. 发明授权

US6001561A Indicator reagents for assays using chemiluminescent electron-rich aryl substituted 1,2-dioxetanes 失效
标题翻译：使用化学发光富电子的芳基取代的1,2-二氧杂环丁烷的测定指示剂
公开(公告)号：US6001561A
公开(公告)日：1999-12-14
申请号：US437347
申请日：1995-05-09
申请人： Nai-Yi Wang , Roger C. Hu
发明人： Nai-Yi Wang , Roger C. Hu
IPC分类号： C07D321/00 , C07D493/04 , C07F7/18 , C07F9/655 , C07F9/6561 , C12Q1/34 , C12Q1/42 , C12Q1/68 , C12Q1/70 , G01N33/533 , G01N33/58 , C09K3/00
CPC分类号： G01N33/582 , C07F7/1856 , C07F9/65512 , C12Q1/34 , C12Q1/42 , G01N33/533 , C12Q2334/00 , Y10S435/968
摘要： Chemiluminescent electron-rich aryl-substituted 1,2-dioxetane compounds are disclosed in which the aryl group is poly-substituted with suitable electron-donating groups such that the light-emitting pattern of the molecule results in a very high luminescent count, thus providing for a sensitive and precise assay for haptens, analytes, polynucleotides and the like. These substituted aryl-containing 1,2-dioxetane compounds can be used as direct labels in an immunoassay or when derivatized with an appropriate leaving group, can be used as a substrate for a enzyme immunoassay. The unusual chemiluminescence of the compounds allows the timing of the luminescent reaction to be exactly controlled.
摘要翻译：公开了化学发光富电子的芳基取代的1,2-二氧杂环丁烷化合物，其中芳基被合适的给电子基团多取代，使得分子的发光图案导致非常高的发光计数，从而提供对于半抗原，分析物，多核苷酸等的敏感和精确的测定。这些取代的含芳基的1,2-二氧杂环丁烷化合物可以在免疫测定中用作直接标记，或者当用合适的离去基团衍生化时，可用作酶免疫测定的底物。化合物的不寻常的化学发光使得准确控制发光反应的时间。

2. 发明授权

US5607625A Chemiluminescent electron-rich aryl-substituted 1,2-dioxetanes 失效
标题翻译：化学发光富电子的芳基取代的1,2-二氧杂环丁烷
公开(公告)号：US5607625A
公开(公告)日：1997-03-04
申请号：US437728
申请日：1995-05-09
申请人： Nai-Yi Wang , Roger C. Hu
发明人： Nai-Yi Wang , Roger C. Hu
IPC分类号： C07D321/00 , C07D493/04 , C07F7/18 , C07F9/655 , C07F9/6561 , C12Q1/34 , C12Q1/42 , C12Q1/68 , C12Q1/70 , G01N33/533 , G01N33/58 , C09K3/00 , G01N21/76
CPC分类号： G01N33/582 , C07F7/1856 , C07F9/65512 , C12Q1/34 , C12Q1/42 , G01N33/533 , C12Q2334/00 , Y10S435/968
摘要： Chemiluminescent electron-rich aryl-substituted 1,2-dioxetane compounds are disclosed in which the aryl group is poly-substituted with suitable electron-donating groups such that the light-emitting pattern of the molecule results in a very high luminescent count, thus providing for a sensitive and precise assay for haptens, analytes, polynucleotides and the like. These substituted aryl-containing 1,2-dioxetane compounds can be used as direct labels in an immunoassay or when derivatized with an appropriate leaving group, can be used as a substrate for a enzyme immunoassay. The unusual chemiluminescence of the compounds allows the timing of the luminescent reaction to be exactly controlled.
摘要翻译：公开了化学发光富电子的芳基取代的1,2-二氧杂环丁烷化合物，其中芳基被合适的给电子基团多取代，使得分子的发光图案导致非常高的发光计数，从而提供对于半抗原，分析物，多核苷酸等的敏感和精确的测定。这些取代的含芳基的1,2-二氧杂环丁烷化合物可以在免疫测定中用作直接标记，或者当用合适的离去基团衍生化时，可用作酶免疫测定的底物。化合物的不寻常的化学发光使得准确控制发光反应的时间。

3. 发明授权

US5264373A Fluorescence polarization immunoassay for tetrahydrocannabinoids 失效
标题翻译：四氢大麻素的荧光偏振免疫测定
公开(公告)号：US5264373A
公开(公告)日：1993-11-23
申请号：US938980
申请日：1992-09-01
申请人： Nai-Yi Wang , Robert E. Dubler , Frank S. Ungemach , Roland L. Walters , Susan A. Thacker
发明人： Nai-Yi Wang , Robert E. Dubler , Frank S. Ungemach , Roland L. Walters , Susan A. Thacker
IPC分类号： C07D311/80 , C07D491/04 , C07D493/10 , C07F7/08 , G01N33/533 , G01N33/542 , G01N33/94
CPC分类号： C07D491/04 , C07D311/80 , C07D493/10 , C07F7/0812 , G01N33/533 , G01N33/542 , G01N33/948 , Y10S436/80 , Y10S436/805 , Y10S436/815 , Y10S530/802
摘要： This disclosure related to a method and reagents for determining tetrahydrocannabinoids (THC) and THC metabolites in a biological fluid such as urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure for determining the presence of THC and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described also provides for novel wash reagent for a THC fluorescence polarization assay.
摘要翻译：本公开涉及生物流体如尿中用于测定四氢大麻素（THC）和THC代谢物的方法和试剂。特别地，本公开内容涉及用于确定THC的存在的荧光偏振免疫测定方法以及在这种方法中用作试剂的新型示踪剂化合物。所述方法还提供了用于THC荧光偏振测定的新型洗涤剂。

4. 发明授权

US5144030A Fluorescene polarization immunoassay for tetrahydrocannabinoids 失效
标题翻译：四氢大麻素的荧光偏振免疫测定
公开(公告)号：US5144030A
公开(公告)日：1992-09-01
申请号：US366266
申请日：1989-06-12
申请人： Nai-Yi Wang , Robert E. Dubler , Frank S. Ungemach , Roland L. Walters , Susan A. Thacker
发明人： Nai-Yi Wang , Robert E. Dubler , Frank S. Ungemach , Roland L. Walters , Susan A. Thacker
IPC分类号： G01N33/53 , C07D311/58 , C07D311/80 , C07D491/04 , C07D491/052 , C07D493/10 , C07D519/00 , C07F7/08 , G01N33/533 , G01N33/542 , G01N33/94
CPC分类号： C07D491/04 , C07D311/80 , C07D493/10 , C07F7/0812 , G01N33/533 , G01N33/542 , G01N33/948
摘要： This disclosure related to a method and reagents for determining tetrahydrocannabinoids (THC) and THC metabolites in a biological fluid such as urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure for determining the presence of THC and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described also provides for novel wash reagent for a THC fluorescence polarization assay.
摘要翻译：本公开涉及生物流体如尿中用于测定四氢大麻素（THC）和THC代谢物的方法和试剂。特别地，本公开内容涉及用于确定THC的存在的荧光偏振免疫测定方法以及在这种方法中用作试剂的新型示踪剂化合物。所述方法还提供了用于THC荧光偏振测定的新型洗涤剂。

5. 发明授权

US06495692B1 Helium-neon excitable reticulocyte dyes derivable from halolepidines 失效
标题翻译：氙 - 氖可激发的网织红细胞染料衍生自卤代哌啶
公开(公告)号：US06495692B1
公开(公告)日：2002-12-17
申请号：US08763022
申请日：1996-12-10
申请人： Nai-Yi Wang , Alex W. C. Yem
发明人： Nai-Yi Wang , Alex W. C. Yem
IPC分类号： C07D21700
CPC分类号： G01N1/30 , C09B23/02 , Y10S436/80 , Y10T436/117497
摘要： A method for synthesizing dyes excitable by a helium-neon laser (excitable at 633 nm) from hololepidines, e. G., 7-halolepidine, which dyes are suitable for the detection and enumeration of reticulocytes in human blood samples. In another aspect, this invention provides a method for immunotyping phenocytes. The method is based on the phenomenon that when dyes of certain structure intercalate into DNA or RNA, the intensity of the dyes increases. The general structure of dyes suitable for use in this invention is shown below: wherein: R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11, R12, R13, Y, and n are as defined in the specification.
摘要翻译：通过氦 - 氖激光（可在633nm激发的）从全萜类可兴奋的染料的方法， G.，7-卤代脒，这些染料适用于人血液样品中网织红细胞的检测和计数。另一方面，本发明提供了一种用于免疫分型的肝细胞的方法。该方法基于当某些结构的染料插入DNA或RNA时，染料的强度增加。适用于本发明的染料的一般结构如下所示：其中：R1，R2，R3，R4 ，R5，R6，R7，R8，R9，R10，R11，R12，R13，Y和n如说明书中所定义。

6. 发明授权

US5427960A Fluorescence polarization assay for cyclosporin A and metabolites and related immunogens and antibodies 失效
标题翻译：环孢菌素A和代谢物及相关免疫原和抗体的荧光偏振测定
公开(公告)号：US5427960A
公开(公告)日：1995-06-27
申请号：US318570
申请日：1994-10-05
申请人： Nai-Yi Wang , Philip P. Wang , Marjorie A. Morrison
发明人： Nai-Yi Wang , Philip P. Wang , Marjorie A. Morrison
IPC分类号： C07K7/64 , G01N33/542 , G01N33/68 , G01N33/94 , G01N33/52 , G01N33/53 , G01N33/58
CPC分类号： G01N33/542 , C07K7/645 , G01N33/68 , G01N33/9493 , Y10S436/805 , Y10S436/815
摘要： The present invention is directed to a fluorescence polarization immunoassay for cyclosporin A and metabolites thereof. The present invention also relates to novel cyclosporin A derivative compounds useful in fluorescence polarization techniques. Included among the novel compounds are cyclosporin A derivatives where the amino acid in the first position is altered. The cyclosporin A derivatives are useful in forming immunogens for raising antibodies specific to cyclosporin A and metabolites thereof.
摘要翻译：本发明涉及环孢菌素A及其代谢物的荧光偏振免疫测定。本发明还涉及用于荧光偏振技术的新型环孢菌素A衍生化合物。包括在新化合物中的是环孢菌素A衍生物，其中第一位置的氨基酸被改变。环孢菌素A衍生物可用于形成用于产生针对环孢菌素A及其代谢物特异性抗体的免疫原。

7. 发明授权

US4569941A Method of using phenyl-alkylene-2-pyridyl derivatives to increase cardiac contractility in a mammal 失效
标题翻译：使用苯基 - 亚烷基-2-吡啶基衍生物增加哺乳动物心脏收缩力的方法
公开(公告)号：US4569941A
公开(公告)日：1986-02-11
申请号：US692602
申请日：1985-01-17
申请人： John T. Suh , Rack H. Chung, deceased , Nai-Yi Wang , Jeffrey N. Barton
发明人： John T. Suh , Rack H. Chung, deceased , Nai-Yi Wang , Jeffrey N. Barton
IPC分类号： C07D207/08 , C07D211/22 , A61K31/445
CPC分类号： C07D207/08 , C07D211/22
摘要： Compounds of the formulae: ##STR1## wherein, Z and Y are each alkylene or oxy-alkylene containing one to about five carbon atoms in the principal chain or said alkylene substituted with OH, alkanoyloxy, alkoxy, mercapto or alkylmercapto;each of R.sub.1, R.sub.2 and R.sub.3 is independently H, alkyl, aryl, halo, alkoxy, alkenyloxy, alkylsulfinyl, alkylsulfonyl, alkylmercapto, cyano, carboxy, carbalkoxy, carboxamido, sulfamoyl, trifluoromethyl, hydroxy, hydroxyalkyl, acyloxy, alkylamino, sulfonylamino, or acylamino; or R.sub.1 and R.sub.2, when taken together, form a methylenedioxy or --O--CO--O--;Ar is heteroaryl, cycloalkyl or ##STR2## wherein, R.sub.1, R.sub.2 and R.sub.3 are as hereindescribed; andR is H, alkyl, cycloalkyl, aryl, aralkyl, alkenyl, alkynyl, carboalkoxy, or CONR.sub.4 R.sub.5 wherein each of R.sub.4 and R.sub.5 is H or alkyl; wherein the total number of carbon atoms in each hydrocarbyl group is up to 10; and ##STR3## wherein, each of R.sub.1 and R.sub.2 is independently H, alkyl, aryl, halo, alkoxy, alkenyloxy, alkylsulfinyl, alkylsulfonyl, alkylmercapto, cyano, carboxy, carbalkoxy, carboxamido, sulfamoyl, trifluoromethyl, hydroxy, hydroxyalkyl, acyloxy, alkylamino, sulfonylamino, or acylamino; or R.sub.1 and R.sub.2, when taken together, form a methylenedioxy, or --O--CO--O--; R.sub.6 is H, alkyl, aryl, halo, alkoxy, alkenyloxy, alkylsulfonyl, alkylmercapto, cyano, carboxy, carbalkoxy, carboxamido, sulfamoyl, trifluoromethyl, hydroxy, hydroxyalkyl, acyloxy, alkylamino, sulfonylamino or acylamino; wherein the total number of carbon atoms in each hydrocarbyl group is up to 10; and acid addition salts thereof have selective cardiotonic, antihypertensive and antiallergic activity.
摘要翻译：下式的化合物：其中Z和Y分别是在主链中含有一至五个碳原子的亚烷基或氧代 - 亚烷基或被OH，烷酰氧基，烷氧基，巯基或烷基巯基取代的所述亚烷基; R 1，R 2和R 3各自独立地是H，烷基，芳基，卤素，烷氧基，烯氧基，烷基亚磺酰基，烷基磺酰基，烷基巯基，氰基，羧基，烷氧基，甲酰胺基，氨磺酰基，三氟甲基，羟基，羟基烷基，酰氧基，烷基氨基，磺酰基氨基或酰氨基; 当R 1和R 2一起形成亚甲二氧基或-O-CO-O-时; Ar是杂芳基，环烷基或者其中R 1，R 2和R 3如以下所示：并且R为H，烷基，环烷基，芳基，芳烷基，烯基，炔基，烷氧羰基或CONR4R5，其中R4和R5各自为H或烷基; 其中每个烃基中的碳原子总数最多为10; 其中，R1和R2各自独立地为H，烷基，芳基，卤素，烷氧基，烯氧基，烷基亚磺酰基，烷基磺酰基，烷基巯基，氰基，羧基，烷氧基，甲酰氨基，氨磺酰基，三氟甲基，羟基，，酰氧基，烷基氨基，磺酰基氨基或酰基氨基; 当R 1和R 2一起形成亚甲二氧基或-O-CO-O-时; R 6是H，烷基，芳基，卤素，烷氧基，烯氧基，烷基磺酰基，烷基巯基，氰基，羧基，烷氧基，甲酰胺基，氨磺酰基，三氟甲基，羟基，羟基烷基，酰氧基，烷基氨基，磺酰基氨基或酰基氨基; 其中每个烃基中的碳原子总数最多为10; 其酸加成盐具有选择性强心药，抗高血压药和抗过敏活性。

8. 发明授权

US07083982B2 Helium-neon excitable reticulocyte dyes derivable from halolepidines 失效
公开(公告)号：US07083982B2
公开(公告)日：2006-08-01
申请号：US10288139
申请日：2002-11-05
申请人： Nai-Yi Wang , Alex W. C. Yem
发明人： Nai-Yi Wang , Alex W. C. Yem
IPC分类号： G01N33/48 , G01N31/00
CPC分类号： G01N1/30 , C09B23/02 , Y10S436/80 , Y10T436/117497
摘要： A method for synthesizing dyes excitable by a helium-neon laser (excitable at 633 nm) from hololepidines, e.g., 7-halolepidine, which dyes are suitable for detection and enumeration of reticulocytes in human blood samples. In another aspect, the invention provides a method for immunotyping phenocytes. The method is based on the phenomenon that when dyes of certain structure intercalate into DNA or RNA, the intensity of the dye increases. Dyes suitable for this invention can be described as having (a) a first heterocyclic moiety, (b) a second heterocyclic moiety, and (c) a linking group that connects the first and second heterocyclic moieties. Both the first and second heterocyclic moieties must contain at least two rings, preferably fused together. The dye is characterized by conjugation, whereby the first moiety is ethylenically conjugated to the second moiety.

9. 发明授权

US6001659A Assays using chemiluminescent electron-rich aryl-substituted 1,2-dioxetanes 失效
标题翻译：使用化学发光富电子的芳基取代的1,2-二氧杂环丁烷的测定
公开(公告)号：US6001659A
公开(公告)日：1999-12-14
申请号：US437342
申请日：1995-05-09
申请人： Nai-Yi Wang , Roger C. Hu
发明人： Nai-Yi Wang , Roger C. Hu
IPC分类号： C07D321/00 , C07D493/04 , C07F7/18 , C07F9/655 , C07F9/6561 , C12Q1/34 , C12Q1/42 , C12Q1/68 , C12Q1/70 , G01N33/533 , G01N33/58
CPC分类号： G01N33/582 , C07F7/1856 , C07F9/65512 , C12Q1/34 , C12Q1/42 , G01N33/533 , C12Q2334/00 , Y10S435/968
摘要： Chemiluminescent electron-rich aryl-substituted 1,2-dioxetane compounds are disclosed in which the aryl group is poly-substituted with suitable electron-donating groups such that the light-emitting pattern of the molecule results in a very high luminescent count, thus providing for a sensitive and precise assay for haptens, analytes, polynucleotides and the like. These substituted aryl-containing 1,2-dioxetane compounds can be used as direct labels in an immunoassay or when derivatized with an appropriate leaving group, can be used as a substrate for a enzyme immunoassay. The unusual chemiluminescence of the compounds allows the timing of the luminescent reaction to be exactly controlled.
摘要翻译：公开了化学发光富电子的芳基取代的1,2-二氧杂环丁烷化合物，其中芳基被合适的给电子基团多取代，使得分子的发光图案导致非常高的发光计数，从而提供对于半抗原，分析物，多核苷酸等的敏感和精确的测定。这些取代的含芳基的1,2-二氧杂环丁烷化合物可以在免疫测定中用作直接标记，或者当用合适的离去基团衍生化时，可用作酶免疫测定的底物。化合物的不寻常的化学发光使得准确控制发光反应的时间。

10. 发明授权

US4632929A Method of hypertensive treatment using phenyl-alkylene-2-pyridyl derivatives 失效
标题翻译：使用苯基 - 亚烷基-2-吡啶基衍生物的高血压治疗方法
公开(公告)号：US4632929A
公开(公告)日：1986-12-30
申请号：US788175
申请日：1985-09-23
申请人： John T. Suh , Rack H. Chung, deceased , Nai-Yi Wang , Jeffrey N. Barton
发明人： John T. Suh , Rack H. Chung, deceased , Nai-Yi Wang , Jeffrey N. Barton
IPC分类号： C07D207/08 , C07D211/22 , A61K31/445
CPC分类号： C07D207/08 , C07D211/22
摘要： Compounds of the formulae: ##STR1## wherein, Z and Y are each alkylene or oxy-alkylene containing one to about five carbon atoms in the principal chain or said alkylene substituted with OH, alkanoyloxy, alkoxy, mercapto or alkylmercapto;each of R.sub.1, R.sub.2 and R.sub.3 is independently H, alkyl, aryl, halo, alkoxy, alkenyloxy, alkylsulfinyl, alkylsulfonyl, alkylmercapto, cyano, carboxy, carbalkoxy, carboxamido, sulfamoyl, trifluoromethyl, hydroxy, hydroxyalkyl, acyloxy, alkylamino, sulfonylamino, or acylamino; or R.sub.1 and R.sub.2, when taken together, form a methylenedioxy or --O--CO--O--;Ar is heteroaryl, cycloalkyl or ##STR2## wherein, R.sub.1, R.sub.2 and R.sub.3 are as hereindescribed; and R is H, alkyl, cycloalkyl, aryl, aralkyl, alkenyl, alkynyl, carboalkoxy, or CONR.sub.4 R.sub.5 wherein each of R.sub.4 and R.sub.5 is H or alkyl; wherein the total number of carbon atoms in each hydrocarbyl group is up to 10; and ##STR3## wherein, each of R.sub.1 and R.sub.2 is independently H, alkyl, aryl, halo, alkoxy, alkenyloxy, alkylsulfinyl, alkylsulfonyl, alkylmercapto, cyano, carboxy, carbalkoxy, carboxamido, sulfamoyl, trifluoromethyl, hydroxy, hydroxyalkyl, acyloxy, alkylamino, sulfonylamino, or acylamino; or R.sub.1 and R.sub.2, when taken together, form a methylenedioxy, or --O--CO--O--; R.sub.6 is H, alkyl, aryl, halo, alkoxy, alkenyloxy, alkylsulfonyl, alkylmercapto, cyano, carboxy, carbalkoxy, carboxamido, sulfamoyl, trifluoromethyl, hydroxy, hydroxyalkyl, acyloxy, alkylamino, sulfonylamino or acylamino; wherein the total number of carbon atoms in each hydrocarbyl group is up to 10; and acid addition salts thereof have selective cardiotonic, antihypertensive and antiallergic activity.
摘要翻译：下式的化合物：其中Z和Y分别是在主链中含有一至五个碳原子的亚烷基或氧代 - 亚烷基或被OH，烷酰氧基，烷氧基，巯基或烷基巯基取代的所述亚烷基; R 1，R 2和R 3各自独立地是H，烷基，芳基，卤素，烷氧基，烯氧基，烷基亚磺酰基，烷基磺酰基，烷基巯基，氰基，羧基，烷氧基，甲酰胺基，氨磺酰基，三氟甲基，羟基，羟基烷基，酰氧基，烷基氨基，磺酰基氨基或酰氨基; 当R 1和R 2一起形成亚甲二氧基或-O-CO-O-时; Ar是杂芳基，环烷基或者其中R 1，R 2和R 3如以下所示：并且R为H，烷基，环烷基，芳基，芳烷基，烯基，炔基，烷氧羰基或CONR4R5，其中R4和R5各自为H或烷基; 其中每个烃基中的碳原子总数最多为10; 其中，R1和R2各自独立地为H，烷基，芳基，卤素，烷氧基，烯氧基，烷基亚磺酰基，烷基磺酰基，烷基巯基，氰基，羧基，烷氧基，甲酰氨基，氨磺酰基，三氟甲基，羟基，，酰氧基，烷基氨基，磺酰基氨基或酰基氨基; 当R 1和R 2一起形成亚甲二氧基或-O-CO-O-时; R 6是H，烷基，芳基，卤素，烷氧基，烯氧基，烷基磺酰基，烷基巯基，氰基，羧基，烷氧基，甲酰胺基，氨磺酰基，三氟甲基，羟基，羟基烷基，酰氧基，烷基氨基，磺酰基氨基或酰基氨基; 其中每个烃基中的碳原子总数最多为10; 其酸加成盐具有选择性强心药，抗高血压药和抗过敏活性。

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